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A Simplified Approach for Olive (<i>Olea europaea</i> L.) Genotyping and Cultivars Traceability
Istituto Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
Istituto Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
Istituto Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
Department of Biotechnology, Agricultural University of Athens, Athens, Greece
Istituto Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
- 1 Istituto Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
- 2 Istituto Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
- 3 Istituto Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
- 4 Department of Biotechnology, Agricultural University of Athens, Athens, Greece
- 5 Istituto Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milano, Italy
American Journal of Plant Sciences·Volume 08 (2017)·Pages 3475–3489·Published 4 December 2017·DOI10.4236/ajps.2017.813234
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Abstract
The Tubulin Based Polymorphism (TBP) method was used to genotype olive cultivars of different origin and to produce short-size cultivar-specific molecular probes. Both the first and the second intron of the members of the olive β -tubulin gene family were exploited as sources of DNA polymorphism. Compared with the data obtained with the use of a set of 11 SSR markers selected from an Olea europea L. database, TBP is shown to provide similar, if not better, information about the polymorphic content of the olive genomes, releasing, at the same time, a simple and discriminatory DNA barcode specific for any of the analyzed cultivars. Such a barcode is the source for the preparation of variety specific molecular probes.
KeywordsOliveGenotypingTubulin Based PolymorphismSSRsMolecular Probes
- Baldoni, L., Cultrera, N., Mariotti, R., Ricciolini, C., Arcioni, S., Vendramin, G., et al. (2009) A Consensus List of Microsatellite Markers for Olive Genotyping. Molecular Breeding, 24, 213-231. https://doi.org/10.1007/s11032-009-9285-8
- Trujillo, I., Ojeda, M.A., Urdiroz, N.M., Potter, D., Barranco, D., Rallo, L., et al. (2014) Identification of the Worldwide Olive Germplasm Bank of Córdoba (Spain) Using SSR and Morphological Markers. Tree Genet Genomes, 10, 141-155. https://doi.org/10.1007/s11295-013-0671-3
- Corrado, G., Imperato, A., La Mura, M., Perri, E. and Rao, R. (2011) Genetic Diversity among Olive Varieties of Southern Italy and the Traceability of Olive Oil Using SSR Markers. Journal of Horticultural Science and Biotechnology, 86, 461-466. https://doi.org/10.1080/14620316.2011.11512789
- Koehmstedt, A.M., Aradhya, M.K., Soleri, D., Smith, J.L. and Polito. V.S. (2011) Molecular Characterization of Genetic Diversity, Structure, and Differentiation in the Olive (Olea europaea L.) Germplasm Collection of the United States Department of Agriculture. Genetic Resources and Crop Evolution, 58, 519-531. https://doi.org/10.1007/s10722-010-9595-z
- Biton, I., Shevtsov, S., Ostersetzer, O., Mani, Y., Lavee, S., Avidan, B. and Ben-Ari, G. (2012) Genetic Relationships and Hybrid Vigour in Olive (Olea europaea L.) by Microsatellites. Plant Breeding, 131, 767-774. https://doi.org/10.1111/pbr.12001
- Linos, A., Nikoloudakis, N., Katsiotis, A. and Hagidimitrioua, M. (2014) Genetic Structure of the Greek Olive Germplasm Revealed by RAPD, ISSR and SSR Markers. Scientia Horticulturae, 175, 33-43. https://doi.org/10.1016/j.scienta.2014.05.034
- This, P., Jung, A., Boccacci, P., Borrego, J., Botta, R., Costantini, L., et al. (2004) Development of a Standard Set of Microsatellite Reference Alleles for Identification of Grape Cultivars. Theoretical and Applied Genetics, 109, 1448-1458. https://doi.org/10.1007/s00122-004-1760-3
- Hoffman, J.I. and Amos, W. (2005) Microsatellite Genotyping Errors: Detection Approaches, Common Sources and Consequences for Paternal Exclusion. Molecular Ecology, 14, 599-612. https://doi.org/10.1111/j.1365-294X.2004.02419.x
- Mousavi, S., Mariotti, R., Regni, L., Nasini, L., Bufacchi, M., Pandolfi, S., Baldoni, L. and Proietti, P. (2017) The First Molecular Identification of an Olive Collection Applying Standard Single Sequence Repeats and Novel Expressed Sequence. Frontiers in Plant Science, 8, 1283. https://doi.org/10.3389/fpls.2017.01283