Interspecific Hybridization between <i>Arisaema sikokianum</i> and <i>A. serratum</i> (Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons
- 1 Faculty of Agriculture, Kochi University, Nankoku, Japan
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- 8 Faculty of Agriculture, Kochi University, Nankoku, Japan
Abstract
A morphologically intermediate plant between <i>Arisaema sikokianum</i> Franch. et Sav. and <i>A. serratum</i> (Thunb.) Schott has been newly found in Kochi Prefecture, Shikoku, Japan. The putative hybrid has the intermediate morphological characteristics of the parental species. Molecular analysis using PCR-RFLP of internal transcribed spacer (ITS) in nuclear DNA (nrDNA) indicates that the putative hybrid has a combined pattern of the two putative parent species. Moreover, the sequence result of chloroplast DNA (cpDNA) of the putative hybrid was identical to that of <i>A. sikokianum</i>. These results suggest that the putative hybrid is a hybrid between <i>A. sikokianum</i> and <i>A. serratum</i> and that it was formed by interactive gene exchanging via pollens from <i>A. serratum</i> to <i>A. sikokianum</i>. It is the first record of a hybrid between <i>A. sikokianum</i> and <i>A. serratum</i>.
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