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Paclobutrazol on <i>In Vitro</i> Growth and Development of <i>Zygopetalum crinitum</i> Orchid, and on Seedling Acclimatization
Sao Paulo State University—UNESP, Jaboticabal, Brazil
Sao Paulo State University—UNESP, Jaboticabal, Brazil
Federal University of Paraná—UFPR, Jandaia do Sul, Brazil
Sao Paulo State University—UNESP, Jaboticabal, Brazil
Sao Paulo State University—UNESP, Jaboticabal, Brazil
Sao Paulo State University—UNESP, Jaboticabal, Brazil
State University of Londrina—UEL, Londrina, Brazil
Sao Paulo State University—UNESP, Jaboticabal, Brazil
- 1 Sao Paulo State University—UNESP, Jaboticabal, Brazil
- 2 Sao Paulo State University—UNESP, Jaboticabal, Brazil
- 3 Federal University of Paraná—UFPR, Jandaia do Sul, Brazil
- 4 Sao Paulo State University—UNESP, Jaboticabal, Brazil
- 5 Sao Paulo State University—UNESP, Jaboticabal, Brazil
- 6 Sao Paulo State University—UNESP, Jaboticabal, Brazil
- 7 State University of Londrina—UEL, Londrina, Brazil
- 8 Sao Paulo State University—UNESP, Jaboticabal, Brazil
American Journal of Plant Sciences·Volume 09 (2018)·Pages 1029–1036·Published 12 April 2018·DOI10.4236/ajps.2018.95079
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Abstract
In vitro cultivation is a technique that allows the production of great amount of plants. However, significant losses may occur during the acclimatization period ( ex vitro stage). The objective of this study was to evaluate the effect of paclobutrazol, in the Murashige and Skoog culture medium, on growth, development, and survival of Zygopetalum crinitum plants. The experimental design (both in vitro and ex vitro ) was completely randomized with four treatments: three paclobutrazol concentrations (0.5; 1.0; and 1.5 mg L -1 active ingredient) and the control (without PBZ). Morphological changes promoted by the paclobutrazol application were observed in the seedlings; however, it did not result in higher seedling survival rates of the Zygopetalum crinitum orchid.
KeywordsGrowth RegulatorGrowth RetardantTriazolOrchidaceae
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