In Search of Regulators of <i>LeSPL-CNR</i> by South-Western Blotting and Yeast One-Hybrid Library Screening System — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
In Search of Regulators of <i>LeSPL-CNR</i> by South-Western Blotting and Yeast One-Hybrid Library Screening System
Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
,
Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
,
Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
,
Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China
,
Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
1 Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
2 Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
3 Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
4 Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, China
5 Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum . The cnr ( colorless non-ripening ) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits normal fruit ripening. In present study, potential regulators of LeSPL-CNR , which could bind to the specific 286 bp region, were screened via south-western blotting and yeast one-hybrid (Y1H) library screening system. Results indicated that a total of 13 and 19 candidate proteins were acquired respectively, and both ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were identified by two methods. These would provide some information for revealing roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.
Keywords<i>Solanum lycopersicumLeSPL-CNR</i>286 bp RegionSouth-Western BlottingYeast One-Hybrid
Giovannoni, J.J. (2004) Genetic Regulation of Fruit Development and Ripening. Plant Cell, 16, 170-180. https://doi.org/10.1105/tpc.019158
Llorente, B., D’Andrea, L., Rui-Sola, M.A., Botterweg, E., Pulido, P., Andilla, J., Loza-Alvarez, P. and Rodriguez-Concepcion, M. (2016) Tomato Fruit Carotenoid Biosynthesis Is Adjusted to Actual Ripening Progression by a Light-Dependent Mechanism. Plant Journal, 85, 107-119. https://doi.org/10.1111/tpj.13094
Qin, G.Z., Zhu, Z., Wang, W.H., Cai, J.H., Chen, Y., Li, L. and Tian, S.P. (2016) A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening. Plant Physiology, 172, 1596-1611. https://doi.org/10.1104/pp.16.01269
The Tomato Genome Consortium (2012) The Tomato Genome Sequence Provides Insights into Fleshy Fruit Evolution. Nature, 485, 635-641. https://doi.org/10.1038/nature11119
Kudo, T., Kobayashi, M., Terashima, S., Katayama, M., Ozaki, S., Kanno, M., Saito, M., Yokoyama, K., Ohyanagi, H., Aoki, K., Kubo, Y. and Yano, K. (2017) Tomatomics: A Web Database for Integrated Omics Information in Tomato. Plant Cell and Physiology, 58, e8. https://doi.org/10.1093/pcp/pcw207
Cara, B. and Giovannoni, J.J. (2008) Molecular Biology of Ethylene during Tomato Fruit Development and Maturation. Plant Science, 175, 106-113. https://doi.org/10.1016/j.plantsci.2008.03.021
Bapat, V.A., Trivedi, P.K., Ghosh, A., Sane, V.A., Ganapathi, T.R. and Nath, P. (2010) Ripening of Fleshy Fruit: Molecular Insight and the Role of Ethylene. Biotechnology Advances, 28, 94-107. https://doi.org/10.1016/j.biotechadv.2009.10.002
Gapper, N.E., McQuinn, R.P. and Giovannoni, J.J. (2013) Molecular and Genetic Regulation of Fruit Ripening. Plant Molecular Biology, 82, 575-591. https://doi.org/10.1007/s11103-013-0050-3
Mou, W.S., Li, D.D., Bu, J.W., Jiang, Y.Y., Khan, Z.U., Luo, Z.S., Mao, L.C. and Ying, T.J. (2016) Comprehensive Analysis of ABA Effects on Ethylene Biosynthesis and Signaling during Tomato Fruit Ripening. PLoS ONE, 11, e0154072. https://doi.org/10.1371/journal.pone.0154072
Wang, R.H., Yuan, X.Y., Meng, L.H., Zhu, B.Z., Zhu, H.L., Luo, Y.B. and Fu, D.Q. (2016) Transcriptome Analysis Provides a Preliminary Regulation Route of the Ethylene Signal Transduction Component, SlEIN2, during Tomato Ripening. PLoS ONE, 11, e0168287. https://doi.org/10.1371/journal.pone.0168287
Rohrmann, J., McQuinn, R., Giovannoni, J.J., Fernie, A.R. and Tohge, T. (2012) Tissue Specificity and Differential Expression of Transcription Factors in Tomato Provide Hints of Unique Regulatory Networks during Fruit Ripening. Plant Signalign & Behavior, 7, 1639-1647. https://doi.org/10.4161/psb.22264
Arhondakis, S., Bita, C.E., Perrakis, A., Manioudaki, M.E., Krokida, A., Kaloudas, D. and Kalaitzis, P. (2016) In silico Transcriptional Regulatory Networks Involved in Tomato Fruit Ripening. Frontiers in Plant Science, 7, 1234. https://doi.org/10.3389/fpls.2016.01234
Manning, K., Tor, M., Poole, M., Hong, Y.G., Thompson, A.J., King, G.J., Giovannoni, J.J. and Seymour, G.B. (2006) A Naturally Occurring Epigenetic Mutation in a Gene Encoding an SBP-Box Transcription Factor Inhibits Tomato Fruit Ripening. Nature Genetics, 38, 948-952. https://doi.org/10.1038/ng1841
Lin, Z.F., Hong, Y.G. and Yin, M.G. (2008) A Tomato HD-Zip Homeobox Protein, LeHB-1, Plays an Important Role in Floral Organogenesis and Ripening. Plant Journal, 55, 301-310. https://doi.org/10.1111/j.1365-313X.2008.03505.x
Vrebalov, J., Pan, I.L., Arroyo, A.J.M., McQuinn, R., Chung, M., Poole, M., Rose, J., Seymour, G., Grandillo, S., Giovannoni, J. and Irish, V.F. (2009) Fleshy Fruit Expansion and Ripening Are Regulated by the Tomato SHATTERPROOF Gene TAGL1. Plant Cell, 21, 3041-3062. https://doi.org/10.1105/tpc.109.066936
Karlova, R., Rosin, F.M., Busscher-Lange, J., Parapunova, V., Do, P.T., Fernie, A.R., Fraser, P.D., Baxter, C., Angenent, G.C. and De Maagd, R.A. (2011) Transcriptome and Metabolite Profiling Show That APETALA2a Is a Major Regulator of Tomato Fruit Ripening. Plant Cell, 23, 923-941. https://doi.org/10.1105/tpc.110.081273
Dong, T.T., Hu, Z., Deng, L., Wang, Y., Zhu, M.K., Zhang, J.L. and Chen, G.P. (2013) A Tomato MADS-Box Transcription Factor, SlMADS1, Acts as a Negative Regulator of Fruit Ripening. Plant Physiology, 163, 1026-1036. https://doi.org/10.1104/pp.113.224436
Fujisawa, M., Nakano, T., Shima, Y. and Yasuhiro, I. (2013) A Larger-Scale Identification of Direct Targets of the Tomato MADS Box Transcription Factor Ripening Inhibitor Reveals the Regulation of Fruit Ripening. Plant Cell, 25, 371-386. https://doi.org/10.1105/tpc.112.108118
Fujisawa, M., Shima, Y., Nakagawa, H., Kitagawa, M., Kimbara, J., Nakano, T., Kasumi, T. and Ito, Y. (2014) Transcriptional Regulation of Fruit Ripening by Tomato Fruitfull homologs and Associated MADS Box Proteins. Plant Cell, 26, 89-101. https://doi.org/10.1105/tpc.113.119453
Zhu, M.K., Chen, G.P., Zhou, S., Tu, Y., Wang, Y., Dong, T.T. and Hu, Z.L. (2014) A New Tomato NAC (NAM/ATAF1/2/CUC2) Transcription Factor, SlNAC4, Fuctions as a Positive Regulator of Fruit Ripening and Carotenoid Accumulation. Plant Cell and Physiology, 55, 119-135. https://doi.org/10.1093/pcp/pct162
Weng, L., Zhao, F.F., Li, R., Xu, C.J., Chen, K.S. and Xiao, H. (2015) The Zinc Finger Transcription Factor SlZFP2 Negatively Regulates Abscisic Acid Biosynthesis and Fruit Ripening in Tomato. Plant Physiology, 167, 931-949. https://doi.org/10.1104/pp.114.255174
Niederhuth, C.E. and Schmitz, R.J. (2014) Covering Your Bases: Inheritance of DNA Methylation in Plant Genomes. Molecular Plant, 7, 472-480. https://doi.org/10.1093/mp/sst165
Kasai, A. and Harada, T. (2015) Epimutant Induction as a New Plant Breeding Technology. JARQ—Japan Agricultural Research Quarterly, 49, 301-305. https://doi.org/10.6090/jarq.49.301
Liu, R.E., How-Kit, A., Stammitti, L., Teyssier, E., Rolin, D., Mortain-Bertrand, A., Halle, S., Liu, M.C., Kong, J.H., Wu, C.Q., Degraeve-Guibault, C., Chapman, N.H., Maucourt, M., Hodgman, T.C., Tost, J., Bouzayen, M., Hong, Y.G., Seymour, G.B., Giovannoni, J.J. and Gallusci, P. (2015) A DEMETER-Like DNA Demethylase Governs Tomato Fruit Ripening. Proceeding of the National Academy of Science of the United States of America, 112, 10804-10809. https://doi.org/10.1073/pnas.1503362112
Zhong, S., Fei, Z.J., Chen, Y.R., Zheng, Y., Huang, M.Y., Vrebalov, J., McQuinn, R., Gapper, N., Liu, B., Xiang, J., Shao, Y. and Giovannoni, J.J. (2013) Single-Base Resolution Methylomes of Tomato Fruit Development Reveal Epigenome Modifications Associated with Ripening. Nature Biotechnology, 31, 154-161. https://doi.org/10.1038/nbt.2462
Chen, W.W., Kong, J.H., Qin, C., Yu, S., Tan, J.J., Chen, Y.R., Wu, C.Q., Wang, H., Shi, Y., Li, C.Y., Zhang, P.C, Wang, Y., Lai, T.F., Yu, Z.M., Zhang, X., Shi, N.N., Wang, H.Z., Osman, T., Liu, Y.L., Manning, K., Jackson, S., Rolin, D., Zhong, S.L., Seymour, G.B., Gallusci, P. and Hong, Y. (2015) Requirement of Chromomethylase3 for Somatic Inheritance of the Spontaneous Tomato Epimutation Colourless Non-Ripening. Scientific Reports, 5, Article No. 9192. https://doi.org/10.1038/srep09192
Long, M., Millar, D.J., Kimura, Y., Donovan, G., Rees, J., Fraser, P.D., Bramley, P.M. and Bolwell, G.P. (2006) Metabolite Profiling of Carotenoid and Phenolic Pathway in Mutant and Transgenic Lines of Tomato: Identification of a High Antioxidant Fruit Line. Phytochemistry, 67, 1750-1757. https://doi.org/10.1016/j.phytochem.2006.02.022
Orfila, C., Huisman, M.M.H., Willats, W.G.T., van Alebeek, G.W.M., Schols, H.A., Seymour, G.B. and Knox, J.P. (2002) Altered Cell Wall Disassembly during Ripeing of Cnr Tomato Fruit: Implications for Cell Adhesion and Fruit Softening. Planta, 215, 440-447. https://doi.org/10.1007/s00425-002-0753-1
Chen, W.W., Kong, J.H., Lai, T.F., Manning, K., Wu, C.Q., Wang, Y., Qin, C., Li, B., Yu, Z., Zhang, X., He, M.L., Zhang, P.C., Gu, M., Yang, X., Mahammed, A., Li, C.Y., Osman, T., Shi, N.N., Wang, H.Z., Jackson, S., Liu, Y., Gollusci, P. and Hong, Y. (2015) Tuning LeSPL-CNR Expression by SlymiR157 Affects Tomato Fruit Ripening. Scientific Reports, 5, Article No. 7852. https://doi.org/10.1038/srep07852
Kanazawa, A., Inaba. J., Shimura, H., Otagaki, S., Tsukahara, S., Matsuzawa, A., Kim, B.M., Goto, K. and Masuta, C. (2011) Virus-Mediated Efficient of Epigenetic Modifications of Endogenous Genes with Phenotypic Changes in Plants. Plant Journal, 65, 156-168. https://doi.org/10.1111/j.1365-313X.2010.04401.x
Lescot, M., Déhais, P., Thijs, G., Marchal, K., Moreau, Y., De Peer, Y.V., Rouzé, P. and Rombauts, S. (2002) PlantCARE, a Database of Plant Cis-Acting Regulatory Elements and a Portal to Tools for in Silico Analysis of Promoter Sequences. Nucleic Acids Research, 30, 325-327. https://doi.org/10.1093/nar/30.1.325
Qin, G.Z., Wang, Y.Y., Cao, B.H., Wang, W.H. and Tian, S.P. (2012) Unraveling the Regulatory Network of the MADS Box Transcription Factor RIN in Fruit Ripening. Plant Journal, 70, 243-255. https://doi.org/10.1111/j.1365-313X.2011.04861.x
Qin, G.Z., Tian, S.P., Chan, Z.L. and Li, B.Q. (2007) Crucial Role of Antioxidant Proteins and Hydrolytic Enzymes in Pathogenicity of Penicillium expansum: Analysis Based on Proteomic Approach. Molecular & Cellular Proteomics, 6, 425-438. https://doi.org/10.1074/mcp.M600179-MCP200
Jacob, Y., Bergamin, E., Donoghue, M.T.A., Mongeon, V., LeBlanc, C., Voigt, P., Underwood, C.J., Brunzelle, J.S., Michaels, S.D., Reinberg, D., Couture, J.F. and Martienssen, R.A. (2014) Selective Methylation of Histone H3 Variant H3.1 Regulates Heterochromatin Replication. Science, 343, 1249-1253. https://doi.org/10.1126/science.1248357
Tagami, H., Ray-Gallet, D., Almouzni, G. and Nakatani, Y. (2004) Histone H3.1 and H3.3 Complexes Mediate Nucleosome Assembly Pathways Dependent or Independent of DNA Synthesis. Cell, 116, 51-61. https://doi.org/10.1016/S0092-8674(03)01064-X
Chin, D. and Means, A.R. (2000) Calmodulin: A Prototypical Calcium Sensor. Trends in Cell Biology, 10, 322-328. https://doi.org/10.1016/S0962-8924(00)01800-6
Banerjee, J., Magnani, R., Nair, M., Dirk, L.M., BeBolt, S., Maiti, I.B. and Houtz, R.L. (2013) Calmodulin-Mediated Signal Transduction Pathways in Arabidopsis Are Fine-Tuned by Methylation. Plant Cell, 25, 4493-4511. https://doi.org/10.1105/tpc.113.119115
Shima, Y., Kitagawa, M., Fujisawa, M., Nakano, T., Kato, H., Kimbara, J., Kasumi, T. and Ito, Y. (2013) Tomato Fruitefull Homologues Act in Fruit Ripening via Forming MADS-Box Transcription Factor Complexes with RIN. Plant Molecular Biology, 82, 427-438. https://doi.org/10.1007/s11103-013-0071-y
Fischer, G. and Schmid, F.X. (1990) The Mechanism of Protein Folding: Implications of in Vitro Refolding Models for de Novo Protein Folding and Translocation in the Cell. Biochemistry, 29, 2205-2212. https://doi.org/10.1021/bi00461a001
Saha, A., Connelly, S., Jiang, J.J., Zhuang, S.H., Amador, D.T., Phan, T., Pilz, R.B. and Boss, G.R. (2014) Akt Phosphorylation and Regulation of Transketolase Is a Nodal Point for Amino Acid Control of Purine Synthesis. Molecular Cell, 55, 264-276. https://doi.org/10.1016/j.molcel.2014.05.028