Non-food lignocellulosic crops with both high biomass yields and superior adaptation to marginal lands have significant potential as biofuel feedstocks that can replace fossil fuels. Deployment of dedicated crops into single biofuels, however, has been reduced by conversion technology costs and low petroleum prices. Integrated biorefinery strategies, in which value-added coproducts are generated in conjunction with biofuels, by comparison offer opportunities to overcome this economic disadvantage. The objective of this research was to evaluate succinic acid accumulation across candidate lignocellulosic feedstocks. Feedstock entries included pearl millet x napiergrass hybrids (“PMN”; Pennisetum glaucum [L.] R. Br. × P. purpureum Schumach.), napiergrass ( P. purpureum Schumach.), annual sorghum ( Sorghum bicolor [L.] Moench), pearl millet ( P. glaucum [L.] R. Br.), perennial sorghum ( Sorghum spp .), switchgrass ( Panicum virgatum L.), sunn hemp ( Crotalaria juncea L.), giant miscanthus ( Miscanthus × giganteus J. M. Greef & Deuter) and energy cane ( Saccharum spp . L.). Replicated field plots, as well as an independent greenhouse trial, were characterized for succinic acid content. The PMN, napiergrass, sunn hemp and energy cane entries had greater ( P ≤ 0.05) succinic acid yields, up to 556 kg·ha-1, in field trials. Napiergrass and PMN entries similarly had higher succinic acid yields under greenhouse conditions; however, irrigation treatments did not alter succinic acid accumulation in this study. Napiergrass, PMN, and energy cane thus are promising biorefinery feedstocks.
KeywordsBiofuelsBiorefinerySuccinic Acid
Werpy, T., Petersen, G., Aden, A., Bozell, J., Holladay, J., White, J., Manheim, A., Eliot, D., Lasure, L. and Jones, S. (2004) Top Value Added Chemicals from Biomass. Volume 1—Results of Screening for Potential Candidates from Sugars and Synthesis Gas. DTIC Document.
Pinazo, J.M., Domine, M.E., Parvulescu, V. and Petru, F. (2015) Sustainability Metrics for Succinic Acid production: A Comparison between Biomass-Based and Petrochemical Routes. Catalysis Today, 239, 17-24. https://doi.org/10.1016/j.cattod.2014.05.035
Bradfield, M.F., Mohagheghi, A., Salvachúa, D., Smith, H., Black, B.A., Dowe, N., Beckham, G.T. and Nicol, W. (2015) Continuous Succinic Acid Production by Actinobacillus succinogenes on Xylose-Enriched Hydrolysate. Biotechnology for Biofuels, 8, 181. https://doi.org/10.1186/s13068-015-0363-3
Du, H., Wang, Z., Yu, W. and Huang, B. (2012) Metabolic Responses of Hybrid Bermudagrass to Short-Term and Long-Term Drought Stress. Journal of the American Society for Horticultural Science, 137, 411-420.
Hanna, W.W., Chaparro, C.J., Mathews, B.W., Burns, J.C., Sollenberger, L.E. and Carpenter, J.R. (2004) Perennial Pennisetums. In: Moser, L.E., Burson, B.L. and Sollenberger, L.E., Eds., Warm-Season (C4) Grasses, Agronomy Monograph 45, American Society of Agronomy, Crop Science Society of America and Soil Science Society of America, Madison, 503-535.
Woodard, K.R. and Prine, G.M. (1991) Forage Yield and Nutritive Value of Elephantgrass as Affected by Harvest Frequency and Genotype. Agronomy Journal, 83, 541-546. https://doi.org/10.2134/agronj1991.00021962008300030005x
Knoll, J.E., Anderson, W.F., Strickland, T.C., Hubbard, R.K. and Malik, R. (2012) Low-Input Production of Biomass from Perennial Grasses in the Coastal Plain of Georgia, USA. BioEnergy Research, 5, 206-214. https://doi.org/10.1007/s12155-011-9122-x
Rengsirikul, K., Ishii, Y., Kangvansaichol, K., Sripichitt, P., Punsuvon, V., Vaithanomsat, P., Nakamanee, G. and Tudsri, S. (2013) Biomass Yield, Chemical Composition and Potential Ethanol Yields of 8 Cultivars of Napiergrass (Pennisetum purpureum Schumach.) Harvested 3-Monthly in Central Thailand. Journal of Sustainable Bioenergy Systems, 3, 107-112. https://doi.org/10.4236/jsbs.2013.32015
Mwendia, S.W., Yunusa, I.A.M., Sindel, B.M., Whalley, R.D.B. and Kariuki, I.W. (2017) Assessment of Napier Grass Accessions in Lowland and Highland Tropical Environments in East Africa: Productivity and Forage Quality. Experimental Agriculture, 53, 27-43. https://doi.org/10.1017/S001447971600003X
Harlan, J.R. and de Wet, J.M.J. (1971) Toward a Rational Classification of Cultivated Plants. Taxon, 20, 509-517. https://doi.org/10.2307/1218252
Hanna, W.W., Baltensperger, D.D. and Seetharam, A. (2004) Pearl Millet and Other Millets. In: Moser, L.E., Burson, B.L. and Sollenberger, L.E., Eds., Warm-Season (C4) Grasses, Agronomy Monograph 45, American Society of Agronomy, Crop Science Society of America and Soil Science Society of America, Madison, 537-560.
Basavaraj, G., Rao, P.P., Bhagavatula, S. and Ahmed, W. (2010) Availability and Utilization of Pearl Millet in India. SAT eJournal, 8, 1-6. http://ejournal.icrisat.org/Volume8/IMPI/Availability_and_utilization.pdf
Jahansouz, M.R., Afshar, R.K., Heidari, H. and Hashemi, M. (2014) Evaluation of Yield and Quality of Sorghum and Millet as Alternative Forage Crops to Corn under Normal and Deficit Irrigation Regimes. Jordan Journal of Agricultural Sciences, 10, 699-715. https://doi.org/10.12816/0031747
Dowling, C.D., Burson, B.L., Foster, J.L., Tarpley, L. and Jessup, R.W. (2013) Confirmation of Pearl Millet-Napiergrass Hybrids Using EST-Derived Simple Sequence Repeat (SSR) Markers. American Journal of Plant Sciences, 4, 1004-1012. https://doi.org/10.4236/ajps.2013.45124
Osgood, R.V., Hanna, W.W. and Tew, T.L. (1997) Hybrid Seed Production of Pearl Millet × Napiergrass Triploid Hybrids. Crop Science, 37, 998-999. https://doi.org/10.2135/cropsci1997.0011183X003700030049x
Jessup, R.W. and Dowling, C.D. (2015) “Seeded-Yet-Sterile” Perennial Grasses: Towards Sustainable and Non-Invasive Biofuel Feedstocks. In: Quinn, L.D., Matlaga, D.P. and Barney, J.N., Eds., Bioenergy and Biological Invasions: Ecological, Agronomic and Policy Perspectives on Minimizing Risk, CABI, Boston, 97-112.
Woodard, K.R. and Prine, G.M. (1993) Dry Matter Accumulation of Elephantgrass, Energycane, and Elephantmillet in a Subtropical Climate. Crop Science, 33, 818-824. https://doi.org/10.2135/cropsci1993.0011183X003300040038x
Gupta, S.C. and Mhere, O. (1997) Identification of Superior Pearl Millet by Napier Hybrids and Napiers in Zimbabwe. African Crop Science Journal, 5, 229-237. https://doi.org/10.4314/acsj.v5i3.27840
Parrish, D.J. and Fike, J.H. (2005) The Biology and Agronomy of Switchgrass for Biofuels. Critical Reviews in Plant Sciences, 24, 423-459. https://doi.org/10.1080/07352680500316433
Kiniry, J.R., Cassida, K.A., Hussey, M.A., Muir, J.P., Ocumpaugh, W.R., Read, J.C., Reed, R.L., Sanderson, M.A., Venuto, B.C. and Williams, J.R. (2005) Switchgrass Simulation by the ALMANAC Model at Diverse Sites in the Southern US. Biomass and Bioenergy, 29, 419-425. https://doi.org/10.1016/j.biombioe.2005.06.003
Sanderson, M.A., Reed, R.L., McLaughlin, S.B., Wullschleger, S.D., Conger, B.V., Parrish, D.J., Wolf, D.D., Taliaferro, C., Hopkins, A.A., Ocumpaugh, W.R. and Hussey, M.A. (1996) Switchgrass as a Sustainable Bioenergy Crop. Bioresource Technology, 56, 83-93. https://doi.org/10.1016/0960-8524(95)00176-X
Muir, J.P., Sanderson, M.A., Ocumpaugh, W.R., Jones, R.M. and Reed, R.L. (2001) Biomass Production of “Alamo” Switchgrass in Response to Nitrogen, Phosphorus, and Row Spacing. Agronomy Journal, 93, 896-901. https://doi.org/10.2134/agronj2001.934896x
Guretzky, J.A., Biermacher, J.T., Cook, B.J., Kering, M.K. and Mosali, J. (2011) Switchgrass for Forage and Bioenergy: Harvest and Nitrogen Rate Effects on Biomass Yields and Nutrient Composition. Plant and Soil, 339, 69-81. https://doi.org/10.1007/s11104-010-0376-4
Heaton, E., Voigt, T. and Long, S.P. (2004) A Quantitative Review Comparing the Yields of Two Candidate C4 Perennial Biomass Crops in Relation to Nitrogen, Temperature and Water. Biomass and Bioenergy, 27, 21-30. https://doi.org/10.1016/j.biombioe.2003.10.005
Pyter, R., Voigt, T., Heaton, E., Dohleman, F. and Long, S. (2007) Growing Giant Miscanthus in Illinois. http://miscanthus.illinois.edu/wp-content/uploads/growersguide.pdf
Pyter, R., Heaton, E., Dohleman, F., Voigt, T. and Long, S. (2009) Agronomic Experiences with Miscanthus x giganteus in Illinois, USA. In: Mielenz, J.R. Ed., Biofuels: Methods and Protocols, Humana Press, Totowa, 41-52. https://doi.org/10.1007/978-1-60761-214-8_3
Kiniry, J.R., Johnson, M.V.V., Bruckerhoff, S.B., Kaiser, J.U., Cordsiemon, R.L. and Harmel, R.D. (2012) Clash of the Titans: Comparing Productivity via Radiation Use Efficiency for Two Grass Giants of the Biofuel Field. BioEnergy Research, 5, 41-48. https://doi.org/10.1007/s12155-011-9116-8
Kering, M.K., Butler, T.J., Biermacher, J.T. and Guretzky, J.A. (2012) Biomass Yield and Nutrient Removal Rates of Perennial Grasses under Nitrogen Fertilization. BioEnergy Research, 5, 61-70. https://doi.org/10.1007/s12155-011-9167-x
Palmer, I.E., Gehl, R.J., Ranney, T.G., Touchell, D. and George, N. (2014) Biomass Yield, Nitrogen Response, and Nutrient Uptake of Perennial Bioenergy Grasses in North Carolina. Biomass and Bioenergy, 63, 218-228. https://doi.org/10.1016/j.biombioe.2014.02.016
Legendre, B.L. and Burner, D.M. (1995) Biomass Production of Sugarcane Cultivars and Early-Generation Hybrids. Biomass and Bioenergy, 8, 55-61. https://doi.org/10.1016/0961-9534(95)00014-X
Knoll, J.E., Anderson, W.F., Richard Jr., E.P., Doran-Peterson, J., Baldwin, B., Hale, A.L. and Viator, R.P. (2013) Harvest Date Effects on Biomass Quality and Ethanol Yield of New Energycane (Saccharum hyb.) Genotypes in the Southeast USA. Biomass and Bioenergy, 56, 147-156. https://doi.org/10.1016/j.biombioe.2013.04.018
Salassi, M.E., Brown, K., Hilbun, B.M., Deliberto, M.A., Gravois, K.A., Mark, T.B. and Falconer, L.L. (2014) Farm-Scale Cost of Producing Perennial Energy Cane as a Biofuel Feedstock. BioEnergy Research, 7, 609-619. https://doi.org/10.1007/s12155-013-9390-8
Shields, S. and Boopathy, R. (2011) Ethanol Production from Lignocellulosic Biomass of Energy Cane. International Biodeterioration & Biodegradation, 65, 142-146. https://doi.org/10.1016/j.ibiod.2010.10.006
Fedenko, J.R., Erickson, J.E., Woodard, K.R., Sollenberger, L.E., Vendramini, J.M., Gilbert, R.A., Helsel, Z.R. and Peter, G.F. (2013) Biomass Production and Composition of Perennial Grasses Grown for Bioenergy in a Subtropical Climate across Florida, USA. BioEnergy Research, 6, 1082-1093. https://doi.org/10.1007/s12155-013-9342-3
Rooney, W.L., Blumenthal, J., Bean, B. and Mullet, J.E. (2007) Designing Sorghum as a Dedicated Bioenergy Feedstock. Biofuels, Bioproducts and Biorefining, 1, 147-157. https://doi.org/10.1002/bbb.15
Pedersen, J.F. and Rooney, W.L. (2004) Sorghums. In: Moser, L.E., Burson, B.L. and Sollenberger, L.E., Eds., Warm-Season (C4) Grasses, Agronomy Monograph 45, American Society of Agronomy, Crop Science Society of America and Soil Science Society of America, Madison, 1057-1079.
Porter, K.S., Axtell, J.D., Lechtenberg, V.L. and Colenbrander, V.F. (1978) Phenotype, Fiber Composition, and in Vitro Dry Matter Disappearance of Chemically Induced Brown Midrib (bmr) Mutants of Sorghum. Crop Science, 18, 205-208. https://doi.org/10.2135/cropsci1978.0011183X001800020002x
Propheter, J.L., Staggenborg, S.A., Wu, X. and Wang, D. (2010) Performance of Annual and Perennial Biofuel Crops: Yield during the First Two Years. Agronomy Journal, 102, 806-814. https://doi.org/10.2134/agronj2009.0301
Bin, J. (1983) Utilization of Green Manure for Raising Soil Fertility in China. Soil Science, 135, 65. https://doi.org/10.1097/00010694-198301000-00013
Rotar, P.P. and Joy, R.J. (1983) “Tropic Sun” Sunn Hemp; Crotalaria juncea L. http://hdl.handle.net/10125/15089
Mansoer, Z., Reeves, D.W. and Wood, C. (1997) Suitability of Sunn Hemp as an Alternative Late-Summer Legume Cover Crop. Soil Science Society of America Journal, 61, 246-253. https://doi.org/10.2136/sssaj1997.03615995006100010034x
Balkcom, K.S. and Reeves, D.W. (2005) Sunn-Hemp Utilized as a Legume Cover Crop for Corn Production. Agronomy Journal, 97, 26-31. https://doi.org/10.2134/agronj2005.0026
Schomberg, H.H., Martini, N.L., Diaz-Perez, J.C., Phatak, S.C., Balkcom, K.S. and Bhardwaj, H.L. (2007) Potential for Using Sunn Hemp as a Source of Biomass and Nitrogen for the Piedmont and Coastal Plain Regions of the Southeastern USA. Agronomy Journal, 99, 1448-1457. https://doi.org/10.2134/agronj2006.0294
Cantrell, K.B., Bauer, P.J. and Ro, K.S. (2010) Utilization of Summer Legumes as Bioenergy Feedstocks. Biomass and Bioenergy, 34, 1961-1967. https://doi.org/10.1016/j.biombioe.2010.08.005
Li, Y.H., Woo, S.H., Choi, D.H. and Cho, E.H. (2015) Succinate Causes α-SMA Production through GPR91 Activation in Hepatic Stellate Cells. Biochemical and Biophysical Research Communications, 463, 853-858. https://doi.org/10.1016/j.bbrc.2015.06.023