Markers for Rapid Evaluation of Virus Resistance for TYLCV in Tomato, ZYMV and PRSV-W in Zucchini and LMV in Lettuce and Hybrid Seeds in Pumpkin — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Markers for Rapid Evaluation of Virus Resistance for TYLCV in Tomato, ZYMV and PRSV-W in Zucchini and LMV in Lettuce and Hybrid Seeds in Pumpkin
Instituto Agronômico-APTA, Campinas, Brazil
,
Instituto Agronômico-APTA, Campinas, Brazil
,
Hortec Sementes Ltda, Bragança Paulista, Brazil
,
Hortec Sementes Ltda, Bragança Paulista, Brazil
,
Hortec Sementes Ltda, Bragança Paulista, Brazil
,
CNPq/PIBITI Scholarship Training, Campinas, Brazil
,
CNPq/PIBITI Scholarship Training, Campinas, Brazil
,
CNPq/PIBITI Scholarship Training, Campinas, Brazil
,
CNPq/PIBITI Scholarship Training, Campinas, Brazil
1 Instituto Agronômico-APTA, Campinas, Brazil
2 Instituto Agronômico-APTA, Campinas, Brazil
3 Hortec Sementes Ltda, Bragança Paulista, Brazil
4 Hortec Sementes Ltda, Bragança Paulista, Brazil
5 Hortec Sementes Ltda, Bragança Paulista, Brazil
6 CNPq/PIBITI Scholarship Training, Campinas, Brazil
7 CNPq/PIBITI Scholarship Training, Campinas, Brazil
8 CNPq/PIBITI Scholarship Training, Campinas, Brazil
9 CNPq/PIBITI Scholarship Training, Campinas, Brazil
Screening for the source of virus resistance in horticultural plants or specific characterization as hybridization, through symptoms, requires time and depends on the weather and knowledge of plant characteristics. So, it is important to develop specific gene markers to allow rapid diagnosis by PCR. Markers were developed based on sequences homology comparison of susceptible and resistant plants provided by HORTEC SEEDS in tomato for <i> Tomato yellow leaf curl virus </i> (TYLCV) by the resistance gene <i> Ty- </i> 1, in zucchini for <i> Zucchini yellow mosaic virus </i> (ZYMV) and <i> Papaya ringspot virus </i> estirpe watermelon (PRSV-W), and in lettuce for <i> Lettuce mosaic virus </i> (LMV). Fragments of 249 bp were amplified only by resistant plants to TYLCV as the hybrids 2648 and Aguamiel, and not for varieties as Santa Cruz or Carina. It were observed for ZYMV the amplification of 791 bp by the resistant hybrid Px7051 and not for the susceptible cultivar La Belle; for PRSV-W using the same zucchini plants the amplification of 650 bp for susceptible and 750 bp for resistant; for LMV the 421 bp amplification only for the resistant cultivar Brasil 303 and not for susceptible Babá de Verão. Finally, it was observed that primers PK47F/R were able to check the Cabotiá seed hybrids of pumpkin Jabras.
Pereira-Carvalho, R.C., Juan, A., Díaz-Pendón, J.A., Fonseca, M.E.N., Boiteux, L.S., Fernández-Muñoz, R., Moriones, E. and Resende, R.O. (2015) Recessive Resistance Derived from Tomato cv. Tyking-Limits Drastically the Spread of Tomato Yellow Leaf Curl Virus. Viruses, 7, 2518-2533. https://doi.org/10.3390/v7052518
Pérez de Castro, A., Blanca, J.M., Díez, M.J. and Vinãls, F.N. (2007) Identification of a CAPS Marker Tightly Linked to the Tomato Yellow Leaf Curl Disease Resistance Gene Ty-1 in Tomato. European Journal of Plant Pathology, 117, 347-356. https://doi.org/10.1007/s10658-007-9103-2
Ferro, D.D.X. (2013) Studies with the locus Ty-1 of the Tomato and Search for New Molecular Markers for Tolerance to Tomato Severe Rugose Vírus. Master Dissertation, University of Brasilia, Brasilia.
Harris, K.R., Ling, K., Wechter, W.P. and Levi, A. (2009) Identification and Utility of Markers Linked to the Zucchini Yellow Mosaic Virus Resistance Gene in Watermelon. Journal of the American Society for Horticultural Science, 134, 529-534.
Apuozzo, C., Manzo, D., Formisano, G. and Ercolano, M.R. (2013) CAPS and HMR Markers for Rapid Detection of ZYMV Resistance Loci in Zucchino. Proceedings of the 57th Italian Society of Agricultural Genetics Annual Congress, Foggia, 16-19 September 2013.
Chen, S.-Y. (2014) Developing the Molecular Markers for Zucchini Yellow Mosaic Virus Resistance in Winter Squash (Cucurbita moschata). International Plant & Animal Genome Conference XXII, 13 January 2014, San Diego, CA, 33 p.
Zraidi, A., Stift, G., Pachner, M., Shojaeiyan, A., Gong, L. and Lelley, T. (2007) A Consensus Map for Cucurbita pepo. Molecular Breeding, 20, 375–388. https://doi.org/10.1007/s11032-007-9098-6
Huang, C.C., Ou, S.S., Lin, C-Z., Chen, S-Y., Chou, J-C. and Ku, H-M. (2014) Simulation Study of Genomic Markers for Zucchini Yellow Mosaic Virus Resistance in Winter Squash (Cucurbita moschata). Crop Environment and Bioinformatics, 11, 26-38.
Pachner, M., Paris, H.S., Winkler, J. and Lelley, T. (2015) Phenotypic and Marker-Assisted Pyramiding of Genes for Resistance to Zucchini Yellow Mosaic Virus in Oilseed Pumpkin (Cucurbita pepo). Plant Breeding, 134, 121-128. https://doi.org/10.1111/pbr.12219
Lihua, G. and Yifei, Z. (2007) Cloning and Analysis of NBS Class Resistant Gene Analogues in Squash (Cucurbita moschata Duch.). Master Thesis, Fujian Agriculture and Forestry University, Fuzhou.
Brotman, Y., Kovalski, I., Dogimont, C., Pitrat, M., Portnoy, V., Katzir, N. and Perl-Treves, R. (2005) Molecular Markers Linked to Papaya Ring Spot Virus Resistance and Fusarium Race 2 Resistance in Melon. Theoretical and Applied Genetics, 110, 337-345.
Teixeira, A.P. and Camargo, L.E.A. (2006) A Molecular Marker Linked to the Prv1 Gene That Confers Resistance to Papaya Ringspot Virus-Type W in Melon. Plant Breeding, 125, 187-190. https://doi.org/10.1111/j.1439-0523.2006.01198.x
Zhang, H.Y., Yuan, F.Z. and Wang, Y-J. (2006) Identification of Molecular Markers Linked to Important Resistant Genes and Construction of Genetic Map in Cucumber RAPDs, RFLPs, AFLPs. Ph.D. Dissertation, Chinese Academy of Agricultural Sciences.
Nicaise, V., German-Retana, S., Sanjuán, R., Dubrana, M-P., Mazier, M., Maisonneuve, B., Candresse, T., Caranta, C. and LeGall, O. (2003) The Eukaryotic Translation Initiation Factor 4E Controls Lettuce Susceptibility to the Potyvirus Lettuce mosaic virus. Plant Physiology, 132, 1272-1282. https://doi.org/10.1104/pp.102.017855
Ge,Y., Li, X., Yang, X.X., Cui, C.S. and Qu, S.P.(2015) Genetic Linkage Map of Cucurbita Maxima with Molecular and Morphological Markers. Genetics and Molecular Research, 14, 5480-5484. https://doi.org/10.4238/2015.May.22.18
Gong, L., Stift, G., Kofler, R., Pachner, M. and Lelley, T. (2008) Microsatellites for the Genus Cucurbita and an SSR-Based Genetic Linkage Map of Cucurbita pepo L. T.A.G., 117, 37-48.
Priori, D., Barbieri, R.L., CastroI, C.M., Oliveira, A.C., Vilella, J.C.B. and Mistura, C.C. (2012) Caracterização molecular de variedades crioulas de abóboras com marcadores microssatélites. Horticultura Brasileira, 30, 499-506. https://doi.org/10.1590/S0102-05362012000300024
Watcharawongpaiboon, N. and Chunwongse, J. (2007) Development of Microsatellite Markers from an Enriched Genomic Library of Pumpkin (Cucurbita moschata L.). Journal of Science and Technology, 29, 1217-1223.
Buscher, N., Zyprian, E. and Blaich, R. (1993) Identification of Grapevine Cultivars by DNA Analyses: Pitfalls of Random Amplified Polymorphic DNA Techniques Using 10 Merprimers. Vitis, 32, 187-188.
Sawazaki, H.E., Duarte, A.P., Fuzatto, M.G., Sawazaki, E., Grandi, S.H.R., Ponte, J.F.P. and Nogueira, L. (2015) Identification and Quantification of Corn, Soybean and Cotton Genetically Modified by Real Time PCR. American Journal of Molecular Biology, 5, 84-93. https://doi.org/10.4236/ajmb.2015.53007
Cardarelli, P., Branquinho, M.R., Ferreira, R.T.B., Cruz, F.P. and Gemal, A.L. (2005) Detection of GMO in Food Products in Brazil: The INCQS Experience. Food Control, 16, 859-866. https://doi.org/10.1016/j.foodcont.2004.07.010
Hall, T.A. (1999) BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Garcia, B.E. and Maxwell, D.P. (2011) Evaluation of the JB1 Marker for Detection of the Ty1 Introgression in Chromosome 6.
Lin, X., Zhang, Y., Kuang, H. and Chen, J. (2013) Frequent Loss of Lineages and Deficient Duplications Accounted for Low Copy Number of Disease Resistance Genes in Cucurbitaceae. B.M.C. Genomics, 14, 335. https://doi.org/10.1186/1471-2164-14-335
Blanca, J., Cañizares, J., Roig, C., Ziarsolo, P., Nuez, F. and Picó, B. (2011) Transcriptome Characterization and High Throughput SSRs and SNPs Discovery in Cucurbita pepo (Cucurbitaceae). B.M.C. Genomics, 12, 104. https://doi.org/10.1186/1471-2164-12-104
Meyer, J.D., Deleu, W., Garcia-Mas, J. and Havey, M.J. (2008) Construction of a Fosmid Library of Cucumber (Cucumis sativus) and Comparative Analyses of the eIF4E and eIF(iso)4E Regions from Cucumber and Melon (Cucumis melo). Molecular Genetics and Genomics, 279, 473-480.
Bui, M. and Liu, Z. (2009) Simple Allele-Discriminating PCR (SAP) for Cost-Effective and Rapid Genotyping and Mapping. Plant Methods, 5, 1. https://doi.org/10.1186/1746-4811-5-1.
Chen, S.X., Du, J.N., Hao, L.N., Wang, C.Y., Chen, Q. and Chang, Y.X. (2012) Identification of Markers Tightly Linked to Tomato Yellow Leaf Curl Disease and Root-Knot Nematode Resistance by Multiplex PCR. Genetics and Molecular Research, 11, 2917-2928. https://doi.org/10.4238/2012.July.10.4