Plants have evolved and perfected a series of light receptors to feel the light at different bands and regulate the expression, modification and interaction of related genes in plants through signal transduction. So far, many photoreceptors have been identified in plants, UVR 8 has recently been identified as a receptor for UV-B light. This paper cloned a WD 40 gene related to UVR 8 protein subunit, named RrRUP 2, based on the Rosa rugose transcriptome data, using Rosa rugose “Zi zhi” as experimental materials. The full length of cDNA of the gene was obtained by RT-PCR and RACE methods. The total length of this gene is 1173 bp, and it encodes 390 amino acids. After bioinformatics analysis, the molecular formula C 3415 H 5659 N 1173 O 1434 S 313 was predicted; the relative molecular weight was 96129.27 Da; the theoretical isoelectric point PI value was 5.00; and its instability index was 47.06. The total average hydrophobic index was 0.750. In the secondary structure of RrRUP 2 protein, there are 10 α -helix, 45 β -helix, 181 Random coil, and 154 Extended strand. Gene Bank Blast results showed that the amino acid sequence encoded by RrRUP 2 was more than 90% homologous with the RUP 2 protein of Rosa chinensis, Fragaria, Malus, Pyrus, P runus, Juglans , Arabidopsis and Tobacco , so it can be inferred that the RrRUP 2 gene is a WD repeat-containing protein. Regarding to fluorescence quantitative expression analysis of RrRUP 2, we find its experssion pattern is corresponded with the accumulation of anthocyanins.
Li, M. (2006) Survey and Quality Evaluation of Shandong Rose Varieties. Shandong University of Traditional Chinese Medicine, Jinan.
Isabels, C., Teresa, C., Laram, C. and Paula, D. (2010) Effect of Photoperiod on Flavonoid Pathway Activity in Sweet Potato (Ipomoea batatas (L.) Lam.) Leaves. Food Chemistry, 118, 384-390. https://doi.org/10.1016/j.foodchem.2009.05.005
Yang, Y.J., Li, Y., Li, X., Guo, X.H., Xiao, X.J. and Tang, D.Y., et al. (2010) Comparative Proteomics Analysis of Light Responses in Cryptochrome1-304 and Columbia Wild-Type 4 of Arabidopsis thaliana. Acta Biochimica et Biophysica Sinica, 40, 27-37. https://doi.org/10.1111/j.1745-7270.2008.00367.x
Ban, Y., Honda, C., Hatsuyama, Y., Igarashi, M., Bessho, H. and Moriguchi, T. (2007) Isolation and Functional Analysis of a MYB Transcription Factor Gene That Is a Key Regulator for The development of Red Coloration in Apple Skin. Plant & Cell Physiology, 48, 958-970. https://doi.org/10.1093/pcp/pcm066
Han, Y.P., Sornkanok, V., Elena, S.G.R. and Korban, S.S. (2012) Introduction of ANR genes into Tobacco Inhibits Expression of Both CHI and DFR genes in Flowers, Leading to Loss of Anthocyanin. Journal of Experimental Botany, 63, 2437-2447. https://doi.org/10.1093/jxb/err415
Albert, N.W., Lewis, D.H., Zhang, H.B., Irving, L.J., Jameson, P.E. and Davies, K.M. (2009) Light-Induced Vegetative Anthocyanin Pigmentation in Petunia. Journal of Experimental Botany, 60, 2191-2202. https://doi.org/10.1093/jxb/erp097
Rockwell, N.C., Su, Y.S. and Lagarias, J.C. (2006) Phytochrome Structure and Signaling Mechanisms. Annual Review of Plant Biology, 57, 837-858. https://doi.org/10.1146/annurev.arplant.56.032604.144208
Georgieva, D.N., Nikolov, P. and Betzel, C. (1998) Steady-State and Time-Resolved Fluorescence of Esperase®: Comparison with the X-Ray Structure in the Region of the Two Tryptophans. Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy, 54, 1109-1116. https://doi.org/10.1016/S1386-1425(98)00026-2
Zhang, J., Stankey, R.J. and Vierstra, R.D. (2013) Structure-Guided Engineering of Plant Phytochrome B with Altered Photochemistry and Light Signaling. Plant Physiology, 161, 1445-1457. https://doi.org/10.1104/pp.112.208892
Heijde, M. and Ulm, R. (2012) UV-B Photoreceptor-Mediated Signalling in Plants. Trends in Plant Science, 17, 230-237. https://doi.org/10.1016/j.tplants.2012.01.007
Tilbrook, K., Arongaus, A.B., Binkert, M., Heijde, M., Yin, R. and Ulm, R. (2013) The UVR8 UV-B Photoreceptor: Perception, Signaling and Response. Arabidopsis Book, 11, e0164. https://doi.org/10.1199/tab.0164
/i>
2
Photomorphogenesis
Anthocyanin
Gene Expression
Brosche, N. and Strid, A. (2010) Molecular Events Following Perception of Ultraviolet-B Radiation by Plants. Physiologia Plantarum, 117, 1-10. https://doi.org/10.1034/j.1399-3054.2003.1170101.x
Ma, G.L.B., Bartels, S., Albert, A. and Ulm, R. (2011) Arabidopsis Map Kinase Phosphatase 1 and Its Target Map Kinases 3 and 6 Antagonistically Determine UV-B Stress Tolerance, Independent of the UVR8 Photoreceptor Pathway. Plant Journal, 68, 727-737. https://doi.org/10.1111/j.1365-313X.2011.04725.x
Kliebenstein, D.J., Lim, J.E., Landry, L.G. and Last, R.L. (2002) Arabidopsis UVR8 Regulates Ultraviolet-B Signal Transduction and Tolerance and Contains Sequence Similarity to Human Regulator of Chromatin Condensation 1. Plant Physiology, 130, 234-243. https://doi.org/10.1104/pp.005041
Ding, W.Z., Zhang, G.L., Fan, L.M., Liu, G.S., Sui, X.Q. and Yuan, Y.B. (2015) Cloning and Bioinformatics Analysis of Apple UVR8. Molecular Plant Breeding, 13, 1779-1785.
Ben-Simhon, Z., Judeinstein, S., Nadler-Hassar, T., Trainin, T., Bar-Ya’Akov, I., Borochov-Neori, H., et al. (2011) A Pomegranate (Punica granatum L.) wd40-Repeat Gene Is a Functional Homologue of Arabidopsis ttg1, and Is Involved in the Regulation of Anthocyanin Biosynthesis during Pomegranate Fruit Development. Planta, 234, 865-881. https://doi.org/10.1007/s00425-011-1438-4
Lloyd, A., Brockman, A., Aguirre, L., Campbell, A., Bean, A., Cantero, A., et al. (2017) Advances in the MYB-bHLH-WD Repeat (MBW) Pigment Regulatory Model: Addition of a WRKY Factor and Co-Option of an Anthocyanin MYB for Betalain Regulation. Plant & Cell Physiology, 58, 1431-1441. https://doi.org/10.1093/pcp/pcx075
Yang, T., Liang, D., Guan, J., Zhu, T., Xia, H. and Lu, X. (2017) Cloning and Expression Analysis of uvr8 (uvr8) in Sweet Cherry. Genomics and Applied Biology, No. 4, 1563-1569.
Wang, W., Yang, D. and Feldmann, K.A. (2011) Efo1 and Efo2, Encoding Putative Wd-Domain Proteins, Have Overlapping and Distinct Roles in the Regulation of Vegetative Development and Flowering of Arabidopsis. Journal of Experimental Botany, 62, 1077. https://doi.org/10.1093/jxb/erq336
Gruber, H., Heijde, M., Heller, W., Albert, A., Seidlitz, H.K. and Ulm, R. (2010) Negative Feedback Regulation of Uv-B Induced Photomorphogenesis and Stress Acclimation in Arabidopsis. Proceedings of the National Academy of Sciences of the United States, 107, 20132-20137. https://doi.org/10.1073/pnas.0914532107
Heijde, M., Binkert, M., Yin, R., Aresorpel, F., Rizzini, L., Van, E.D.S., et al. (2013) Constitutively Active uvr8 Photoreceptor Variant in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 110, 20326-20331. https://doi.org/10.1073/pnas.1314336110
Yang, H.Q., Wu, Y.J., Tang, R.H., Liu, D., Liu, Y. and Cashmore, A.R. (2000) The c Termini of Arabidopsis Cryptochromes Mediate a Constitutive Light Response. Cell, 103, 815-827. https://doi.org/10.1016/S0092-8674(00)00184-7
Lau, O.S. and Deng, X.W. (2012) The Photomorphogenic Repressors cop1 and det1: 20 Years Later. Trends in Plant Science, 17, 584-593. https://doi.org/10.1016/j.tplants.2012.05.004
Saijo, Y., Sullivan, J.A., Wang, H., Yang, J., Shen, Y., Rubio, V., et al. (2003) The Cop1-Spa1 Interaction Defines a Criti. Genes & Development, 17, 2642-2647. https://doi.org/10.1101/gad.1122903
Deng, X.W., Caspar, T. and Quail, P.H. (1992) Cop1: A Regulatory Locus Involved in Light-Controlled Development and Gene Expression in Arabidopsis. The Plant Cell, 4, 1507-1518. https://doi.org/10.1105/tpc.4.12.1507