Cloning and Characterization of a Mitogen-Activated Protein Kinase Gene 84<i>KMPK</i>14 in Hybrid Poplar (<i>Populus alba</i> × <i>P. glandulosa</i> cv. “84K”) — Oak Academic Publishing
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Cloning and Characterization of a Mitogen-Activated Protein Kinase Gene 84<i>KMPK</i>14 in Hybrid Poplar (<i>Populus alba</i> × <i>P. glandulosa</i> cv. “84K”)
Forestry College of Shandong Agricultural University, Tai’an, China
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Forestry College of Shandong Agricultural University, Tai’an, China
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Forestry College of Shandong Agricultural University, Tai’an, China
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Forestry College of Shandong Agricultural University, Tai’an, China
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Shandong Institute of Pomology, Tai’an, China
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Forestry College of Shandong Agricultural University, Tai’an, China
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Forestry College of Shandong Agricultural University, Tai’an, China
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Forestry College of Shandong Agricultural University, Tai’an, China
1 Forestry College of Shandong Agricultural University, Tai’an, China
2 Forestry College of Shandong Agricultural University, Tai’an, China
3 Forestry College of Shandong Agricultural University, Tai’an, China
4 Forestry College of Shandong Agricultural University, Tai’an, China
5 Shandong Institute of Pomology, Tai’an, China
6 Forestry College of Shandong Agricultural University, Tai’an, China
7 Forestry College of Shandong Agricultural University, Tai’an, China
8 Forestry College of Shandong Agricultural University, Tai’an, China
Mitogen-activated protein kinases (MAPKs) are important components in signal transduction modules which play crucial roles in regulation of many biological processes in plants. Although genome-wide analysis of MAPK and MAPKK family has been carried out in poplar species, few data about the biological function analysis of this gene family are available to date. In this study, a group C MAPK gene 84 <i> KMPK </i> 14 was cloned from hybrid poplar ( <i> Populus alba </i> × <i> P. glandulosa </i> cv. “ 84K ” ). It contained a typical protein kinase domain, a conserved TEY-motif and an atypical conserved common docking (CD) domain. Sequence alignment revealed that 84 <i> KMPK </i> 14 was the most homologous to <i> Populus trichocarpa PtMPK </i> 14 . Expression analysis indicated that the transcript of 84 <i> KMPK </i> 14 in roots and young leaves was higher than that in other tissues. The expression of 84 <i> K MPK </i> 14 was down-regulated by low or high temperature and was induced by H 2 O 2 significantly. It was suppressed by drought and salinity stresses slightly one hour after treatment and then increased quickly three hours after treatment. These results indicated that 84 <i> KMPK </i> 14 may be involved in environmental stresses, which provides basis for further characterization of the physiological analysis on this gene.
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