Influence of Isolated PGPR Rhizobacteria in Central and Northern Benin on Maize Germination and Greenhouse Growth — Oak Academic Publishing
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Influence of Isolated PGPR Rhizobacteria in Central and Northern Benin on Maize Germination and Greenhouse Growth
Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
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Centre de Recherches Agricoles d’Agonkanmey, Institut National des Recherches Agricoles du Bénin, Cotonou, Bénin
,
Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
,
Département de Biologie Végétale, Laboratoire de Microbiologie et des Technologies Alimentaires, Cotonou, Bénin
,
Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
,
Faculté des Science Agronomique (FSA), Laboratoire de Biomathématique et d’Estimation Forestière, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
,
Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
,
Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
,
Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
,
Centre de Recherches Agricoles Sud, Institut National des Recherches Agricoles du Bénin, Attogon, Bénin
,
Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
1 Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
2 Centre de Recherches Agricoles d’Agonkanmey, Institut National des Recherches Agricoles du Bénin, Cotonou, Bénin
3 Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
4 Département de Biologie Végétale, Laboratoire de Microbiologie et des Technologies Alimentaires, Cotonou, Bénin
5 Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
6 Faculté des Science Agronomique (FSA), Laboratoire de Biomathématique et d’Estimation Forestière, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
7 Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
8 Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
9 Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
10 Centre de Recherches Agricoles Sud, Institut National des Recherches Agricoles du Bénin, Attogon, Bénin
11 Département de Biochimie et de Bio-logie Cellulaire, Faculté des Sciences et Techniques (FAST), Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey-Calavi (UAC), Cotonou, Bénin
The objective of the study was to evaluate the effects of nine plant growth-promoting rhizobacteria (PGPR) alone or in combination on maize seed germination and seedling growth under laboratory and greenhouse conditions. The germination and growth tests were carried out in square petri dishes and pots. Maize seeds were inoculated with suspension of 10 8 CFU/ml of rhizobacteria. The experimental device was a random block of 16 treatments with four repetitions. Germination test results showed that seeds inoculated with PGPR including the control induced good germination in the range of 93.75% to 100%. The vigor index and root length of the seeds treated with <i> Bacillus panthothenicus </i> were significantly improved by 76.64% and 58.86%, respectively, while the maximal lengths of the seedlings were obtained with <i> Pseudomonas cichorii </i> with an increase of 118.95%. In greenhouse experience, data demonstrated that <i> Serratia marcescens </i> better improved the leaf area, height and underground biomass, respectively by 58.83%, 108.43%, and 59.16% as compared to the control. The highest fresh aerial biomass and air dry matter was obtained with plants treated only with <i> Pseudomonas putida </i> . These results show the potential to use such rhizobacteria as biofertilizers to improve maize productivity in Benin.
Ahmadi, N., Chantereau, J., Hekimian Le Thève, C., Marchand, J.L. and Ouendeba, B (2002) The Cereals. In: Memento of Agriculture, CIRAD-GRET, 780-792
Cairns, J.E., Probe, K., Zaidi, P.H., Verhulst, N., Mahuku, G., Babu, R., Nair, S., Das, B., Govaerts, B. and Vinayan, M. (2012) Maize Production in a Changing Climate: Impacts, Adaptation, and Mitigation Strategies. Advances in Agronomy, 114, 1-58.
Toléba, S.M., Biaou, G., Saïdou, A. and Zannou, A. (2015) Functioning of the Maize Sector in Benin. CBRST Papers, Vol. 1, Cotonou, 73-35-73.
WAAPP/CNS-Corn (2012) Ratings Agronomic and Socio-Economic Systems of maize Production in the Agroecological Zones of Benin. Research Project Document, 62 p.
INSAE (2010) Interim Results of the Rgph 4. Cotonou.
WFP (2014) Global Analysis of Vulnerability, Food Security and Nutrition (AGVSAN).
USDA (2017). https://quickstats.nass.usda.gov/
Azontondé, A.H., Igué, A.M. and Dagbenonbakin, G. (2010) Benin Soil Fertility Map by Agroecological Zone of Benin. Final Report, Benin.
MAEP (2017) MAEP Plan Stratégique de Développement du Secteur Agricole (PSDSA) 2025 et Plan National d’Investissements Agricoles et de Sécurité Alimentaire et Nutritionnelle PNIASAN 2017-2021. Cotonou.
Alamri, R., Ben, A., Ben, H.A. and Labidi, S. (2016) Installation of a Biofertilizer Production unit. Seminar Report II, National Agronomic Institute of Tunisia. http://www.memoireonline.com/01/16/9399
Alalaoui, A.C. (2007) Mineral Fertilization of Crops: Major Nutrients (Nitrogen, Potassium and Phosphorus). Monthly Bulletin of Information and Liaison, 155, 1-4.
Okon, Y. and Hadar, Y. (1987) Microbial Inoculants as Crop-Yield Enhancers. Critical Reviews in Biotechnology, 6, 61-85. https://doi.org/10.3109/07388558709086985
Timmusk, S., Paalme, V., Pavlicek, T., Bergquist, J., Vangala, A., Danilas, T. and Nevo, E. (2011) Bacterial Distribution in the Rhizosphere of wild Barley under Contrasting Microclimates. PLoS ONE, 6, e17968.
Kumar, V.K., Reddy, M.S., Kloepper, J.W., Lawrence, K.S., Zhou, X.G., Groth, D.E., Zhang, S., Sudhakara, R.R., Wang, Q., Raju, M.R.B., Krishnam, R., Dilantha, F.W.G., Sudini, H., Du, B. and Miller, M.E. (2011) Commercial Potential of Microbial Inoculants for Sheath Blight Management and Yield Enhancement of Rice. In: Maheshwari, D.K., Ed., Bacteria in Agrobiology: Crop Ecosystems, Springer, Berlin, 237-264.
Ma, Y., Oliveira, R.S., Freitas, H. and Zhang, C. (2016) Biochemical and Molecular Mechanisms of Plant-Microbe-782 Metal Interactions: Relevance for Phytoremediation. Frontiers in Plant Science, 7, 918. https://doi.org/10.3389/fpls.2016.00918
Biswas, J.C., Ladha, J.K. and Dazzo, F.B. (2000) Rhizobia Inoculation Improves Nutrient Uptake and Growth of Lowland Rice. Soil Science Society of America Journal, No. 64, 1644-1650. https://doi.org/10.2136/sssaj2000.6451644x
Mehrvarz, S., Chaichi, M.R. amd Alikhani, H.A. (2008) Effects of Phosphate Solubilizing Microorganisms and Phosphorus Chemical Fertilizers on Yield and Yield Components (Hordum vulgare L). American-Eurasian Journal of Agricultural Environmental Sciences, 3, 822-828.
Majeed, A., Abbasi, M.K., Hameed, S. and Imran, N. (2015) Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Wheat Hizo-Sphere and Their Effect on Plant Growth Promoting. Frontiers in Microbiology, 6, 198. https://doi.org/10.3389/fmicb.2015.00198
Cakamackci, R., Dönmez, M.F. and Erdogan, ü. (2007) The Effect of Plant Growth Promotes Rhizobacteria on Barely Seedling Growth, Nutrient Uptake, Sum Soil Properties, and Bacterial Counts. Turkish Journal of Agriculture and Forestry, 31, 189-199.
Agbodjato, N.A., Noumavo, P.A., Adjanohoun, A., Agbessi, L. and Baba-Moussa, L. (2016) Synergistic Effects of Plant Growth Promoting Rhizobacteria and Chitosan in Vitro Germination, Greenhouse Growth, and Nutrient Uptake of Maize (Zea mays L.). Biotechnology Research International, 2016, Article ID: 7830182. https://doi.org/10.1155/2016/7830182
Yallou, C.G., Aihou, K., Adjanohoun, A., Baco, M.N., Sanni, O.A. and Amadou, L. (2010) Répertoire des Variétés de Maïs Vulgarisées au Bénin: Document Technique d’Information et de vulgarisation. Dépôt légal N° 4920, 4e Trimestre, Bibliothèque Nationale du Bénin, 19 p.
Agbodjato, N.A., Noumavo, P.A., Baba-Moussa, F., Salami, H.A., Sina, H., Sezan, A., Bankole, H., Adjanohoun, A. and Baba-Moussa, L. (2015) Characterization of Potential Plant Growth Promoting Rhizobacteria Isolated from Maize (Zea mays L.) in Central and Northern Benin (West Africa). Applied and Environmental Soil Science, 2015, Article ID: 901656.
Guiraud, J. and Galzy, P. (1994) Contrôle Microbiologique dans les Industries Agroalimentaires (2ème eds) Doin.
Wahyudi, A.T., Astuti, R.P., Widyawati, A., Meryandini, A. and Nawangsih, A.A. (2011) Characterization of Bacillus sp. Strains Isolated from Rhizosphere of Soybean Plants for Their Use as Potential Plant Growth for Promoting Rhizobacteria. Journal of Microbiology and Antimicrobials, 3, 34-40.
Aparna, Y. and Sarada, J. (2012) Molecular Characterization and Phylogenetic Analysis of Serratia sp-YAJS An Extracellular Dnase Producer Isolated from Rhizosphere Soil.
Govindappa, M.R.V., Ravishankar, S. and Lokesh (2011) Screening of Pseudomonas Fluorescens Isolates for Biological Control of Macrophomina phaseolina Root-Rot of Safflower. African Journal of Agricultural Research, 6, 6256-6266. https://doi.org/10.5897/AJAR10.1017
Niranjan, R.S., Shetty, N.P. and Shetty, H.S. (2004) Seed Bio-Priming with Pseudomonas Fluorescens Isolates Enhances Growth of Pearl Millet Plants and Induces Resistance against Downy Mildew. International Journal of Pest Management, 50, 41-48. https://doi.org/10.1080/09670870310001626365
Yadav, J., Verma, J.P. and Tiwari, K.N. (2010) Effect of Plant Growth Promoting Rhizobacteria on Seed Germination and Plant Growth Chickpea (Cicer arietinum L.) under Vitro Conditions. Biological Forum: An International Journal, 2, 15-18.
Neelesh, K.A., Arvind, K. and Hirdesh, A. (2011) Physiological and Bio-Chemical Changes during Seed Deterioration in Aged Seeds of (Zea mays L.). American Journal of Plant Physiology, 6, 28-35. https://doi.org/10.3923/ajpp.2011.28.35
Khayatnezhad, M. and Gholamin, R. (2011) Effects of Salt Stress Levels on Five Maize (Zea mays L.) Cultivars at Germination Stage. African Journal of Biotechnology, 10, 12909-12915. https://doi.org/10.5897/AJB11.1568
Abdul-Baki, A.A. and Anderson, J.D. (1973) Vigor Determination in Soy Bean Seed by Multiplecriteria. Crop Science, 13, 630-633. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
Ashrafuzzaman, M., Hossen, F.A., Ismail, M.R., Hoque, M.A., Islam, Z.M., Shahidullah, S.M. and Meon, S. (2009) Efficiency of Plant Growth-Promoting Rhizobacteria (PGPR) for the Enhancement of Rice Growth. African Journal of Biotechnology, 8, 1247-1252.
Etèka, A.C. (2005) Comtribution des jachère manioc dans l’amélioration du rendement des cultures et du prélèvement des nutriments: Cas de la succession culturale manioc-maïs au Centre du Bénin. Thèse de DEA, FSA/UAC, Bénin, 107 p.
Kumar, V., Bharti, A., Negi, Y.K., Gusain, O., Pandey, P. and Bisht, G.S. (2012) Screening of Actinomycetes from Earthworm Castings for Their Antimicrobial Activity and Industrial Enzymes. Brazilian Journal of Microbiology, 205-214. https://doi.org/10.1590/S1517-83822012000100022
Olsen, S.R. and Sommers, L.E. (1982) Phosphorus. In: Page, A.L., Ed., Methods of Soil Analysis Part 2 Chemical and Microbiological Properties, American Society of Agronomy, Soil Science Society of America, Madison, 403-430.
Bremner, J.M. (1996) Nitrogen-Total. In: Sparks, D.L., Ed., Methods of Soil Analysis, Part 3, Soil Science Society of America, Madison, 1085-1121.
Knudsen, D., Peterson, G.A. and Pratt, P. (1982) Lithium, Sodium and Potassium. In: Page, A.L., Ed., Methods of Soil Analysis, American Society of Agronomy, Madison, 225-246.
Thomas, G.W. (1982) Exchangeable Cation. In: Page, A.L., Miller, R.H. and Keeney, D.R., Eds., Methods of Soil Analyse, Madison, 154-157.
Ruget, F. and Bonhomme, C.M. (1996) Estimation Simple de la surface foliaire de plantes de maïs en croissance. Agronomie, 16, 553-562. https://doi.org/10.1051/agro:19960903
Nkonge, C. and Balance, G.M. (1982) A Sensitive Colorimetric Procedure for Nitrogen Determination in Micro-Kjeldahl Digests. Journal of Agricultural and Food Chemistry, 30, 416-420. https://doi.org/10.1021/jf00111a002
Ones, J.B.J. and Case, V.W. (1990) Sampling, Handling and Analyzing Plant Tissue Samples. In: Westrman, R.L., Ed., Soil Testing and Plant Analysis, 3rd Edition, SSSA Book Series No. 3, Madison Wisconsin, 389-427.
Murphy, J. and Riley, J.P. (1962) A Modified Single Solution Method for the Determination of Phosphate in Natural Waters. Analytica Chimica Acta, 27, 31-36. https://doi.org/10.1016/S0003-2670(00)88444-5
Igué, M.A., Saïdou, A., Balogoun, I., Ezui, G., Youl, S., Kpagbin, G., Mando, A. and Sogbédji, J.M. (2016) Détermination Des Formules D’engrais Minéraux Et Organiques Sur Deux Types De Sols Pour Une Meilleure Productivité De Maïs (Zea mays L.) Dans La Commune De Banikoara (Nord-Est Du Bénin). European Scientific Journal, 12, 16 p.
Noumavo, A.P., Kochoni, E., Didagbé, Y.O., Adjanohoun, A., Allagbé, M., Sikirou, R., Gachomo, E.W., Kotchoni, O.S. and Baba-Moussa, L. (2013) Effect of Different Plant Growth Promoting Rhizobacteria on Maize Seed Germination and Seedling Development. American Journal of Plant Sciences, 4, 1013-1021. https://doi.org/10.4236/ajps.2013.45125
Omar, S.A. (1998) The Role of Rock-Phosphate-Solubilizing Fungi and Vesicular Arbusular-Mycorrhiza (VAM) in Growth of Wheat Plants Fertilized with Rock Phosphate. Word Journal of Microbiology and Biotechnology, 14, 211-2118. https://doi.org/10.1023/A:1008830129262
Cassán, F., Vanderleyden, J. and Spaepen, S. (2014) Physiological and Agro-Nomic Aspects of Phytohormone Production by Model Plant-Growth-Promoting Rhizobacteria (PGPR) Belonging to the Genus Azospirillum. Journal of Plant Growth Regulation, 33, 440-459. https://doi.org/10.1007/s00344-013-9362-4
Koo, S.Y. and Cho, K.S. (2009) Isolation and Characterization of a Plant-Growth Promoting Rhizobacterium, Serratia sp. SY5. Journal of Microbiology and Biotechnology, 19, 1431-1438.
Khalid, A., Arshad, M. and Zahir, Z.A. (2004) Screening Plant Growth Promoting Rhizobacteria for Improving Growth and Yield of Wheat. Journal of Applied Microbiology, 96, 473-480. https://doi.org/10.1046/j.1365-2672.2003.02161.x
Vacheron, J., Desbrosses, G., Bouffaud, M.L., Touraine, B., Moene-Loccoz, Y., Muller, D., Legendre, L., Wisniewski-Dye, F. and Prigent-Combaret, C. (2013) Plant Growth Promoting Rhizobacteria and Root System. Frontiers in Plant Science, 4, 356. https://doi.org/10.3389/fpls.2013.00356
Kifle, M.H. and Laing, M.D. (2016) Effects of Selected Diazotrophs on Maize Growth. Frontiers in Plant Science, 7, 1429. https://doi.org/10.3389/fpls.2016.01429
Chowdhury, A.R. and Sengpta, C. (2016) Isolation and Characterization of Plant Growth Promoting Rhizobacteria (pgpr) from Agricultural Field and Their Potential Role on Germination and Growth of Spinach. IJCAS, 6, 128-131.
Prasad, J.K., Gupta, S.K. and Raghuwanshi, R. (2017) Screening Multifunctional Plant Growth Promoting Rhizobacteria Strains for Enhancing Seed Germination in Wheat (Triticum aestivum L.). International Journal of Agricultural Research, 12, 64-72. https://doi.org/10.3923/ijar.2017.64.72
Babana, A.H., Kassogué, A., Fané, R., Nantoumé, D., Ouattara, D., Maiga, K. and Dao, S. (2018) A Malian Native Plant Growth Promoting Actinomycetes Based Biofertilizer Improves Maize Growth and Yield. Symbiosis, 75, 267-275. https://doi.org/10.1007/s13199-018-0555-2
Viruel, E., Erazzú, L.E., Calsina, L.M., Ferrero, M.A., Lucca, M.E. and Siñeriz, F. (2014) Inoculation of Maize with Phosphate Solubilizing Bacteria: Effect on Plant Growth and Yield. Journal of Soil Science and Plant Nutrition, 14, 819-831. https://doi.org/10.4067/S0718-95162014005000065
Chakraborty, U.B.N., Chakraborty, A.P. and Chakraborty (2010) Influence of Serratia marcescens TRS-1 on Growth Promotion and Induction of Resistance in Camellia sinensis against Fomes lamaoensis. Journal of Plant Interactions, 4, 261-271. https://doi.org/10.1080/17429140903551738
Devi, K.A., Pandey, P. and Sharma, G.D. (2016) Plant Growth-Promoting Endophyte Serratia marcescens AL2-16. Enhances the Growth of Achyranthes aspera L., a Medicinal Plant. Hayati Journal of Biosciences, 23, 173-180. https://doi.org/10.1016/j.hjb.2016.12.006
Matteoli, F.P., Passarelli-Araujoa, H., Reisb, R.J.A., Rochab, O.L, Souzac, M.E, Aravindd, L., Olivaresb, L.F. and Venancio, M.T. (2018) Genome Sequencing and Assessment of Plant Growth-Promoting Properties of a Serratia marcescens Strain Isolated from Vermicompost. CC-BY-NC-ND 4.0 International License. https://doi.org/10.1101/288084
Riefler, M., Ondrej, N., Miroslav, S. and Thomas, S. (2006) Arabidopsis Cytokinin Receptor Mutants Reveal Functions in Shoot Growth, Leaf Senescence, Seed Size, Germination, Root Development, and Cytokinin Metabolism. The Plant Cell, 18, 40-54. https://doi.org/10.1105/tpc.105.037796
Hameeda, B., Harini, G., Rupela, O.P., Wani, S.P. and Reddy, G. (2006a) Growth Promotion of Maize by Phosphate Solubilizing Bacteria Isolated from Composts and Macrofauna. Microbiological Research, 163, 234-242. https://doi.org/10.1016/j.micres.2006.05.009
Agbodjato, N.A., Amogou, O., Noumavo, P.A, Dagbenonbakin, G., Hafiz, A.S., Kamirou, R., Alladé, A.M., Adebayo, O., Baba-Moussa, F., Adjanohoun, A. and Ba-ba-Moussa, L. (2018) Biofertilising, Plant-Stimulating and Biocontrol Potentials of Isolated PGPR Rhizobacteria in Central and Northern Benin. African Journal of Microbiology Research, 12, 664-672.
Mezaache, S. (2012) Localisation des déterminants de la suppression de quelques souches de Pseudomonas isolées de la rhizosphère de la pomme de terre. thèse de doctorat, Université Ferhat ABBAS, Sétif, Algérie, 221.
Selvakumar, G., Mohan, M., Kundu, S., Gupta, A.D., Joshi, P., Nazim, S. and Gupta, H.S. (2008) Cold Tolerance and Plant Growth Promotion Potential of Serratia marcescens Strain SRM (MTCC 8708) Isoled from Frowers of Summer Squash (Curcubita pepo). Letters in Applied Microbiology, 46, 171-175. https://doi.org/10.1111/j.1472-765X.2007.02282.x
Arruda, L.A., Beneduzi, A., Martins, B., Lisboa, C., Lopes, F., Bertolo, L., Maria, P., Passaglia, L. and Vargas, K. (2013) Screening of Rhizobacteria Isolated from Maize (Zea mays L.) in Rio Grande do Sul State (South Brazil) and Analysis of Their Potential to Improve Plant Growth. Applied Soil Ecology, 63, 15-22. https://doi.org/10.1016/j.apsoil.2012.09.001
Namli, A., Mahmood, A., Sevilir, B. and Özkir, E. (2017) Effect of Phosphorus Solubilizing Bacteria on Some Soil Properties, Wheat Yield and Nutrient Contents. Eurasian Journal of Soil Science, 6, 249-258. https://doi.org/10.18393/ejss.293157
Tarafdar, J.C. and Claassen, N. (1988) Organic Phosphorus Compounds as a Phosphorus Source for Higher Plants through the Activity of Phosphates Produced by Plant Roots and Microorganisms. Biology and Fertility of Soils, 5, 308-312. https://doi.org/10.1007/BF00262137
Marschner, H. (1995) Mineral Nutrition of Higher Plants. 2nd Edition, Academic Press, London, 889 p.
Adjanohoun, A., Noumavo, P.A., Sikirou, R., Allagbé, M., Gotoe-chan-Hodonou, H., Dossa, K.K., Yèhouénou, B., Glèlè Kakaï, R. and Baba-Moussa, L. (2012) Effets des rhizobactéries PGPR sur le rendement et les teneurs en ma-croéléments du maïs sur sol ferralitique non dégradé au Sud-Bénin. International Journal of Biological and Chemical Sciences, 6, 279-288.