Combined Action of Copper with IAA on Individual Amino Acids and Microelement in Pods of Two Broad Bean Cultivars

Abstract

It is worthy to mention that the two broad bean cultivars displayed a surprising situation during the fruiting stage of growth. The absolute amount of pods yield was higher in cv. Assiut 125 than in cv. Assiut 84. This situation was in contrast with that in the vegetative stage, where cv. Assiut 84 produced greater vegetative growth than cv. Assiut 125. Although the cv. Assiut 84 was less affected by the highest dose of copper, the cv. Assiut 125 was greatly affected by this dose. However, another surprising situation was recorded when the absolute values of fresh and dry matter of cv. Assiut 125 were much higher than cv. Assiut 84 at any used copper concentration. The fresh matters of the pods of cv. Assiut 84 and cv. Assiut 125 were 7.24 and 10.16 gm at the level of control and 6.69 and 7.59 gm at the level of 350 ppm Cu, respectively. Their dry matters were 1.99 and 3.52 gm at the level of control and 1.77 and 2.13 gm at the level of 350 ppm Cu²⁺in cv. Assiut 84 and cv. Assiut 125, respectively. This contradiction was also extended when these copper-affected cultivars were sprayed by IAA. The cv. Assiut 84 responded slightly to IAA treatment. On the other hand, a considerable increase in the dry matter yield was recorded in the pods of cv. Assiut 125 at any used level of copper when compared to the non-sprayed plants treated by the same copper concentration. At the level of 350 ppm Cu²⁺, the pod yield was 2-fold that of plants treated only by 350 ppm Cu²⁺, without IAA spraying. Thus, even IAA behaved differently in its effect on the crop yield of the two tested broad bean cultivars. There are big and surprising variations in the accumulation of microelements and heavy metals in pods among the two broad bean genotypes, whatever the treatments used. These variations seemed to be complicated which might need further studies. The concentration of amino acids and their individuals also varied among the two cultivars; they increased in cv. Assiut 84 and decreased in cv. Assiut 125. The increased amino acids in cv. Assiut 84 are mostly used as precursors for phytochelatins. For the previous studies, thus the present work was conducted to explain the effect of Cu and interactive effect with IAA on two selected broad bean cultivars Assiut 84 and Assiut 125 during yielding production.