Botanical Aspects, Caffeine Content and Antioxidant Activity of <i>Coffea arabica</i>

Abstract

Brazil is the largest coffee exporter in the world. In addition, it occupies the second position, among the consuming countries of the drink. To investigate the chemical composition and quality of the coffee drink from Coffea arabica species, samples grown in the city of Ourinhos, the third most productive region in the state of Sao Paulo, a study of its properties and characteristics was conducted. The pharmacobotanical characteristics were investigated performed according to usual techniques in these researches through macroscopic and microscopic studies through cross-sections. The oil obtained for analysis was extracted by soxhlet, and the caffeine content was measured for green grains using High Performance Liquid Chromatography (HPLC) using as stationary phase, column C 18 and gradient mobile phase formed by 80% water and 20% methanol. The concentration obtained was 44.983 ± 0.86 μ g/mL. The antioxidant activity was measured in triplicate through the DPPH test of the in natura coffee oil, and presented antioxidant action of EC 50 25.89 ± 1.16 μ g/mL.