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Influence of Agitation Rate on the Growth of MD2 Pineapple Protocorm-Like Bodies and Shoots in Liquid-Shake Culture
Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Kuala Lumpur, Malaysia
Horticulture Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Sintok, Malaysia
Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Kuala Lumpur, Malaysia
School of Food Science and Technology, Universiti Malaysia Terengganu, Kuala Terengganu, Malaysia
- 1 Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Kuala Lumpur, Malaysia
- 2 Horticulture Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Sintok, Malaysia
- 3 Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Kuala Lumpur, Malaysia
- 4 School of Food Science and Technology, Universiti Malaysia Terengganu, Kuala Terengganu, Malaysia
American Journal of Plant Sciences·Volume 10 (2019)·Pages 1233–1238·Published 11 July 2019·DOI10.4236/ajps.2019.107088
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Abstract
The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.
KeywordsPineapple MD2MicropropagationLiquid-Shake CultureAgitation Rate
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