Antimicrobial Activity of Five Yemeni Medicinal Plants against Selected Human Pathogenic Bacteria and Fungi
- 1 Dept. of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana’a, University, Sana’a, Yemen
- 2 Dept. of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana’a, University, Sana’a, Yemen
- 3 Dept. of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana’a, University, Sana’a, Yemen
- 4 Dept. of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana’a, University, Sana’a, Yemen
- 5 Dept. of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana’a, University, Sana’a, Yemen
- 6 Dept. of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana’a, University, Sana’a, Yemen
Abstract
Background : The emergence and spread of antibiotic resistance, as well as the evolution of new strains of disease causing agents, are of great concern to the global health community. Nowadays, reports have indicated that many of healthcare-associated, antimicrobial-resistant bacteria are not only multidrug resistant pathogens but also broadly drug-resistant and pandrug-resistant bacteria . Objective : This study is focused on exploring the antimicrobial properties of five different plants that are commonly being used as traditional medicines in Yemen against Gram positive , Gram negative bacteria and fungi. Methods : The antimicrobial potential of five different plant extracts was screened against selected human pathogenic bacteria and fungi . Methanolic extracts of Dodonaea viscose , Plantago lanceolata , Withania samnifera , Pulicaria crispa , and Rumex nervosus were subjected to a test of their antimicrobial properties by Modified Agar Diffusion and Minimum Inhibitory Concentration. Results: T he results indicated that all the five plants extract showed antibacterial activity against a selected microorganism at a concentration of 6 0 mg/ml . The highest potential was observed in the extract of W . somnifera against S. taphyloccocus aureus , Pseudomonas aeruginosa , Proteus miarbilis and Candida albicans with zone of inhibition of 23 mm, 20.7 mm, 20 mm and 25.3 mm, respectively. R . nervosus also showed the highest MIC against test organisms . Only two medicinal plant Pulicaria crispa , and Rumex nervosus extracts showed non antifungal activity. Conclusion : The experiment confirmed the efficacy of some selected plant extracts as natural antimicrobial and can be potential sources for the synthesis of antibacterial drugs.
- Bhatia, R. and Narain, J.P. (2010) The Growing Challenge of Antimicrobial Resistance in the South-East Asia Region—Are We Losing the Battle? Indian Journal of Medical Research, 132, 482-486.
- Boucher, H.W., Talbot, G.H., Bradley, J.S., Edwards Jr., J.E., Gilbert, D., Rice, L.B., Scheld, M., Spellberg, B. and Bartlett, J. (2009) Bad Bugs, No Drugs: No ESKAPE! An Update from the Infectious Diseases Society of America. Clinical Infectious Diseases, 48, 1-12. https://doi.org/10.1086/595011
- Giamarellou. H. (2010) Multidrug-Resistant Gram-Negative Bacteria: How to Treat and for How Long. International Journal of Antimicrobial Agents, 36, S50-S54. https://doi.org/10.1016/j.ijantimicag.2010.11.014
- Coates, A., Hu, Y., Bax, R. and Page, C. (2002) The Future Challenges Facing the Development of New Antimicrobial Drugs. Nature Reviews Drug Discovery, 1, 895-910. https://doi.org/10.1038/nrd940
- Marasini, B.P., Baral, P., Aryal, P., Ghimire, K.R., Neupane, S., Dahal, N., Singh, A., Ghimire, L. and Shrestha, K. (2015) Evaluation of Antibacterial Activity of Some Traditionally Used Medicinal Plants against Human Pathogenic Bacteria. BioMed Research International, 2015, Article ID: 265425. https://doi.org/10.1155/2015/265425
- Iwu, M.W., Dunca, A.R. and Okunji, C.O. (1999) New Antimicrobials of Plant Origin in. Perspectives on New Crops and New Uses. In: Janick, J., Ed., Plant Breeding Reviews, ASHS Press, Alexandria, VA.
- World Health Organization (2002) WHO Traditional Medicine Strategy. Geneva.
- Borchardt, R., Wyse, D.L., Sheaffer, C.C., Kauppi, K.L., Fulcher, R.G., Ehlke, N.J., Biesboer, D.D. and Bey, R.F. (2008) Antimicrobial Activity of Native and Naturalized Plants of Minnesota and Wisconsin. Journal of Medicinal Plants Research, 2, 98-110.
- Al-Haj, N.A., Mashan, N.I. and Shamudin, M.N. (2009) Antibacterial Activity in Marine Algae Eucheuma denticulatum against Staphylococcus aureus and Streptococcus pyogenes. Research Journal of Biological Sciences, 4, 519-524.
- AL-Haj, N.A., Mashan, N.I., Mariana, N., Shamsudin, M.N., Mohamad, H., Vairappan, C.S. and Sekawi, Z. (2010) Antibacterial Activity of Marine Source Extracts Against Multidrug Resistance Organisms. American Journal of Pharmacology and Toxicology, 5, 95-102. https://doi.org/10.3844/ajptsp.2010.95.102
- Al-Haj, N.A., Hassan, K.Z., Alabed, A.A.A., Al Mahdi, A.Y., Abdsalam, R. and Albawani, S.M (2018) Antibacterial Assays of Malaysian Medicinal Plant Polygonum minus Using Different Extraction Methods. Research Journal of Medical Sciences, 12, 5-11.