Major pathogens causing fruit rots of apple in shelf life in Hangzhou, a city in east China, were identified by rDNA-ITS analysis. Their pathogenicities and stress tolerances were compared as well. Combining with disease symptoms, colonial phenotypes and mycelial microscopic morphology, the fungi were determined as Penicillium expansum , Botrytis cinerea , Botryosphaeria dothidea , Diaporthe phaseolorum , Alternaria alternata and Fusarium acuminatum , respectively . Among them, B. cinerea and B. dothidea showed a higher pathogenicity; B. cinerea and D. phaseolorum were hardly affected by the temperature at a range of 15°C and 25°C; B. cinerea has the highest resistant to Thiabendazole and D. phaseolorum displayed the strongest resistance to Imazalil; and P. expansum was most sensitive to ultraviolet light radiation. The results provide some useful information that helps to combine conventional and alternative control strategies to minimize apple postharvest losses in shelf life.
Morales, H., Marín, S., Ramos, A.J. and Sanchis, V. (2010) Influence of Post-Harvest Technologies Applied during Cold Storage of Apples in Penicillium expansum Growth and Patulin Accumulation: A Review. Food Control, 21, 953-962. https://doi.org/10.1016/j.foodcont.2009.12.016
León-Zapata, M.A.De., Sáenz-Galindo, A., Rojas-Molina, R., Rodríguez-Herrera, Jasso-Cantú, D. and Aguilar, C.N. (2015) Edible Candelilla Wax Coating with Fermented Extract of Tarbush Improves the Shelf Life and Quality of Apples. Food Packaging and Shelf Life, 3, 70-75. https://doi.org/10.1016/j.fpsl.2015.01.001
Mohammed, M., Bridgemohan, P., Mohamed, M.S., Bridgemohan, R.S.H. and Mohammed, Z. (2017) Postharvest Physiology and Storage of Golden Apple (Spondias cythera sonnerat or Spondias dulcis Forst): A Review. Journal of Food Processing & Technology, 8, Article ID: 1000707. https://doi.org/10.4172/2157-7110.1000707
Li, H., Wan, Y.Z., Wang, M., Han, M.Y. and Huo, H.X. (2015) History, Status and Prospects of the Apple Industry in China. Journal of the American Pomological Society, 69, 174-185. https://www.researchgate.net/publication/287202013_Pecan_flavor_changes_during_storage#page=4
Lai, T.F., Bai, X.L., Wang, Y., Zhou, J.Y., Shi, N.N. and Zhou, T. (2015) Inhibitory Effect of Exogenous Sodium Bicarbonate on Development and Pathogenicity of Postharvest Disease Penicillium expansum. Scientia Horticulturae, 187, 108-114. https://doi.org/10.1016/j.scienta.2015.03.010
Liu, J., Sui Y., Wisniewski, M., Droby, S. and Liu, Y.L. (2013) Review: Utilization of Antagonistic Yeasts to Manage Postharvest Fungal Diseases of Fruit. International Journal of Food Microbiology, 167, 153-160. https://doi.org/10.1016/j.ijfoodmicro.2013.09.004
Feliziani, E., Lichter, A., Smilanick, J.L. and Ippolito, A. (2016) Disinfecting Agents for Controlling Fruit and Vegetable Diseases after Harvest. Postharvest Biology and Technology, 122, 53-69. https://doi.org/10.1016/j.postharvbio.2016.04.016
Palou, L., Ali, A., Fallik, E. and Romanazzi, G. (2016) GRAS, Plant- and Animal-Derived Compounds as Alternatives to Conventional Fungicides for the Control of Postharvest Diseases of Fresh Horticultural Produce. Postharvest Biology and Technology, 122, 41-52. https://doi.org/10.1016/j.postharvbio.2016.04.017
Tannous, J., Keller, N.P., Atoui, A., Khoury, A.E., Lteif, R., Oswald, I.P. and Puel, O. (2018) Secondary Metabolism in Penicillium expansum: Emphasis on Recent Advances in Patulin Research. Critical Reviews in Food Science and Nutrition, 58, 2082-2098. https://doi.org/10.1080/10408398.2017.1305945
Barad, S., Sionov, E. and Prusky, D. (2016) Role of Patulin in Postharvest Diseases. Fungal Biology Reviews, 30, 24-32. https://doi.org/10.1016/j.fbr.2016.02.001
Ballester, A.R., Marcet-Houben, M., Levin, E., Sela, N., Selma-Lázaro, C., Carmona, L., Wisniewski, M., Droby, S., González-Candelas, L. and Gabaldón, T. (2015) Genome, Transcriptome, and Functional Analyses of Penicillium expansum Provide New Insights into Secondary Metabolism and Pathogenicity. Molecular Plant-Microbe Interactions, 28, 232-248. https://doi.org/10.1094/MPMI-09-14-0261-FI
Zhou, T., Wang, X.H., Luo, J., Ye, B.S., Zhou, Y.Y., Zhou, L.W. and Lai, T.F. (2018) Identification of Differentially Expressed Genes Involved in Spore Germination of Penicillium expansum by Comparative Transcriptome and Proteome Approaches. Microbiology Open, 7, e562. https://doi.org/10.1002/mbo3.562
Kan, J.A.L., Stassem, J.H.M., Mosbach, A., Lee, T.A.J.V.D., Faino, L., Farmer, A.D., Papasotiriou, D.G., Zhou, S., Seidl, M.F., Cottam, E., EDEL, D., Hahn, M., Schwartz, D.C., Dietrich, R.A., Widdison, S. and Scalliet, G. (2017) A Gapless Genome Sequence of the Fungus Botrytis cinera. Molecular Plant Pathology, 18, 75-89. https://doi.org/10.1111/mpp.12384
Williamson, B., Tudzynski, B., Tudzynski, P. and Kan, J.A.L.V. (2007) Botrytis cinerea: The Cause of Grey Mould Disease. Molecular Plant Pathology, 8, 561-580. https://doi.org/10.1111/j.1364-3703.2007.00417.x
Romanazzi, G., Smilanick, J.L., Feliziani, E. and Droby, S. (2016) Integrated Management of Postharvest Gray Mold on Fruit Crop. Postharvest Biology and Technology, 113, 69-76. https://doi.org/10.1016/j.postharvbio.2015.11.003
Liu, Z.T., Lian, S., Li, B.H., Lu, H.Y., Dong, X.L. and Wang, C.X. (2016) Draft Genome Sequence of Botryosphaeria dothidea, the Pathogen of Apple Ring Rot. American Society for Microbiology Journals, 4, e01142-16. https://doi.org/10.1128/genomeA.01142-16
Tang, W., Ding, Z., Zhou, Z.Q., Wang, Y.Z. and Guo, L.Y. (2012) Phylogenetic and Pathogenic Analyses Show That the Causal Agent of Apple Ring Rot in China Is Botryosphaeria dothidea. Plant Disease, 96, 486-496. https://doi.org/10.1094/PDIS-08-11-0635
Guan, Y.Q., Chang, R.F., Liu, G.J., Wang, Y., Wu, T., Han, Z.H. and Zhang, X.H. (2015) Role of Lenticels and Microcracks on Susceptibility of Apple Fruit to Botryosphaeria dothidea. European Journal of Plant Pathology, 143, 317-330. https://doi.org/10.1007/s10658-015-0682-z
Bai, S.H., Dong, C.H., Li, B.H. and Dai, H.Y. (2013) A PR-4 Gene Identified from Malus domestica Is Involved in the Defense Responses against Botryosphaeria dothidea. Plant Physiology and Biochemistry, 62, 23-32. https://doi.org/10.1016/j.plaphy.2012.10.016
Brown-Rytlewski, D.E. and McManus, P.S. (2000) Virulence of Botryosphaeria dothidea and Botryosphaeria obtusa on Apple and Management of Stem Cankers with Fungicides. Plant Disease, 84, 1031-1037. https://doi.org/10.1094/PDIS.2000.84.9.1031
Fan, K., Wang, J., Fu, L., Li, X.J., Zhang, Y., Zhang, X.D. and Zhai, H. (2016) Sensitive of Botryoshaeria dothidea from Apple to Tebuconazole in China. Crop Protection, 87, 1-5. https://doi.org/10.1016/j.cropro.2016.04.018
Danggomen, A., Visarathanonth, N., Manoch, L. and Piasai, O. (2013) Morphological Studies of Endophytic and Plant Pathogenic Phomopsis liquidambaris and Diaporthe phaseolorum (P. phaseoli Anamorph) from Healthy Plants and Diseased Fruit. Thai Journal of Agricultural Science, 46, 157-164. http://www.thaiagj.org/images/stories/Journal_online/2013/3/07-tj-agr-1013-66-p157-164.pdf
Cui, H., Yu, J.C., Chen, S.H., Ding, M., Huang, X.S., Yuan, J. and She, Z.G. (2017) Alkaloids from the Mangrove Endophytic Fungus Diaporthe phaseolorum SKS019. Bioorganic & Medicinal Chemistry Letters, 27, 803-807. https://doi.org/10.1016/j.bmcl.2017.01.029
Dissanayake, A.J., Liu, M., Zhang, W., Chen, Z., Udayanga, D., Chukeatirote, E., Li X.H., Yan, J.Y. and Dyde, K.D. (2015) Morphological and Molecular Characterization of Diaporthe Species Associated with Grapevine Trunk Disease in China. Fungal Biology, 119, 283-294. https://doi.org/10.1016/j.funbio.2014.11.003
Dissanayake, A.J., Phillips, A.J.L., Hyde, K.D., Yan, J.Y. and Li, X.H. (2017) The Current Status of Species in Diaporthe. Mycosphere, 8, 1106-1156. https://doi.org/10.5943/mycosphere/8/5/5
Scott, P. (2001) Analysis of Agricultural Commodities and Foods for Alternaria Mycotoxins. Journal of AOAC International, 84, 1809-1817.
Xu, Y., Gao, C.W., Li, X.H., He, Y., Zhou, L.T., Pang, G.R. and Sun, S.T. (2013) In Vitro Antifungal Activity of Silver Nanoparticles against Ocular Pathogenic Filamentous Fungi. Journal of Ocular Pharmacology and Therapeutics, 29, 270-274. https://doi.org/10.1089/jop.2012.0155
Gabriel, M.F., Postigo, I., Tomaz, C.T. and Martínez, J. (2016) Alternaria alternata Allergens: Markers of Exposure, Phylogeny and Risk of Fungi-Induced Respiratory Allergy. Environment International, 89-90, 71-80. https://doi.org/10.1016/j.envint.2016.01.003
Li, L., Pan, H., Liu, W., Chen, M.Y. and Zhong, C.H. (2017) First Report of Alternaria alternata Causing Postharvest Rot of Kiwifruit in China. Plant Disease, 101, 1046. https://doi.org/10.1094/PDIS-11-16-1611-PDN
Alam, M.W., Rehman, A., Malik, A.U., Aslam, S., Sarwar, M., Ali, S., Khan, M.A., Hameed, A. and Sarfraz, S. (2018) First Report of Alternaria alternate Causing Postharvest Fruit Rot of Peach in Pakistan. Journal of Plant Pathology, 8, 1. https://doi.org/10.1007/s42161-018-0160-5
Yang, J.L., Sun, C., Zhang, Y.Y., Fu, D., Zheng, X.D. and Yu, T. (2017) Induced Resistance in Tomato Fruit by γ-Aminobutyric Acid for the Control of Alternaria Rot Caused by Alternaria alternata. Food Chemistry, 221, 1014-1020. https://doi.org/10.1016/j.foodchem.2016.11.061
Timmer, L.W., Peever, T.L., Solerl, Z. and Akimitsu, K. (2003) Alternaria Diseases of Citrus-Novel Pathosystems. Phytopathologia Mediterranea, 42, 99-112.
Bertero, A., Spicer, L.J. and Caloni, F. (2018) Fusarium Mycotoxins and in Vitro Species-Specific Approach with Porcine Intestinal and Brain in Vitro Barriers: A Review. Food and Chemical Toxicology, 121, 666-675. https://doi.org/10.1016/j.fct.2018.09.050
Chai, A.L., Li, P.L., Guo, W.T., Li, B.J. and Aisimutuola, P. (2018) First Report of Fusarium acuminatum Wilt in the Broomrape Parasite of Processing Tomato in China. Plant Disease, 102, 676. https://doi.org/10.1094/PDIS-08-17-1244-PDN
Du, M., Ren, X.Y., Sun, Q.H., Wang, Y. and Zhang, R.F. (2012) Characterization of Fusarium spp. Causing Potato Dry Rot in China and Susceptibility Evaluation of Chinese Potato Germplasm to the Pathogen. Potato Research, 55, 173-184. https://doi.org/10.1007/s11540-012-9217-6
Wang, C.W., Ai, J., Fan, S.T., Lv, H.Y., Qin, H.Y., Yang, Y.M. and Liu, Y.X. (2015) Fusarium acuminatum: A New Pathogen Causing Postharvest Rot on Stored Kiwifruit in China. Plant Disease, 99, 1644. https://doi.org/10.1094/PDIS-01-15-0021-PDN
Wang, Y., Wang, C.W., Gao, J. and Yang, L.N. (2016) First Report of Fusarium acuminatum Causing Postharvest Fruit Rot on Stored Vaccinium corymbosum in China. Plant Disease, 100, 2527. https://doi.org/10.1094/PDIS-04-16-0529-PDN
Wang, Y., Guan, Y.M., Lu, B.H. and Gao, J. (2016) First Report of Ginseng (Panax ginseng) Root Rot Caused by Fusarium acuminatum in China. Plant Disease, 100, 525. https://doi.org/10.1094/PDIS-03-15-0273-PDN
Akbar, A., Hussain, S., Ullah, K., Fahim, M. and Ali, G.S. (2018) Detection, Virulence and Genetic Diversity of Fusarium Species Infecting Tomato in Northern Pakistan. PLoS ONE, 13, e0203613. https://doi.org/10.1371/journal.pone.0203613
Braun, H., Woitsch, L., Hetzer, B., Geisen, R., Zange, B. and Schmidt-Heydt, M. (2018) Trichoderma harzianum: Inhibition of Mycotoxin Producing Fungi and Toxin Biosynthesis. International Journal of Food Microbiology, 280, 10-16. https://doi.org/10.1016/j.ijfoodmicro.2018.04.021
Srivastava, M., Shahid, M., Pandey, S., Singh, A., Kumar, V., Gupta, S.J. and Maurya, M. (2014) Trichoderma Genome to Genomics: A Review. Journal of Data Mining in Genomics & Proteomics, 5, 162. https://doi.org/10.4172/2153-0602.1000162
Sharma, V., Salwan, R. and Sharma, P.N. (2016) Differential Response of Extracellular Proteases of Trichoderma harzianum against Fungal Phytopathogens. Current Microbiology, 73, 419-425. https://doi.org/10.1007/s00284-016-1072-2