Phytochemical Screening and Antimicrobial Activity of <i>Desmodium ramosissimum</i> — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Phytochemical Screening and Antimicrobial Activity of <i>Desmodium ramosissimum</i>
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
,
South Agricultural Research Centre, National Agricultural Research Institute of Benin, Attogon, Benin
,
Laboratoire de Biochimie et d’Immunologie Appliquée (LABIA), UFR en Sciences de la vie et de la terre, école Doctorale Sciences et Technologies, Université Ouaga I Pr-Joseph KI-ZERBO, Ouagadougou, Burkina Faso
,
Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
1 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
2 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
3 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
4 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
5 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
6 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
7 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
8 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
9 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
10 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
11 South Agricultural Research Centre, National Agricultural Research Institute of Benin, Attogon, Benin
12 Laboratoire de Biochimie et d’Immunologie Appliquée (LABIA), UFR en Sciences de la vie et de la terre, école Doctorale Sciences et Technologies, Université Ouaga I Pr-Joseph KI-ZERBO, Ouagadougou, Burkina Faso
13 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Cotonou, Benin
Microbial infections threaten the health of the Beninese population. Objectives : To identify bioactive phytochemical groups and evaluate the antimicrobial activity of Desmodium ramosissimum used in traditional medicine in Benin against microbial infections. Methodology & Results : The classic reactions in solution helped highlight alkaloids, anthocyanins, flavonoids, quinones, saponins, steroids, tannins, terpenoids, mucilage, Coumarin, reducing compounds, anthracene, glycosides. The antibacterial activity was evaluated by extracts dilution’s method (ethanol extracts, methanol extracts and hexane extracts). The methanol extract inhibited the growth of all tested germs, with a significant effect on E. coli and S. aureus strains whose MIC values are equal to 1.25 mg/ml. On the contrary, this extract has a low activity on P. vulgaris strain with a MIC of 10 mg/ml. The ethanol extract also inhibited the growth of all the tested strains, but with more intense activity than the methanol extract. The strains of C. albicans and S. oralis were more sensitive to the extract with 0.625 mg/ml for MIC, while S. aureus and P. vulgaris were less sensitive (MIC = 5 mg/ml). The residual ethanol extract exerted inhibition on all microorganisms at a concentration of 20 mg/ml, except the strain of E. foecalis that was resistant. Conclusion & Applications : This study shows that besides its food virtues of this plant, there are antibacterial and antifungal properties.
Traoré, Y., Ouattara, K., Yéo, D., Doumbia, I. and Coulibaly, A. (2012) Recherche des activités antifongique et antibactérienne des feuilles d’Annona senegalensis Pers. (Annonaceae). Journal of Applied Biosciences, 58, 4234-4242.
N’tcha, C., Sina, H., Peter, A., Kayodé, P., Gbenou, J.D. and BabaMoussa, L. (2017) Antimicrobial Activity and Chemical Composition of (Kpete-Kpete): A Starter of Benign Traditional Beer Tchoukoutou. BioMed Research International, 2017, Article ID: 6582038. https://doi.org/10.1155/2017/6582038
Balakrishna, M., Khaki, S.S., Karuna, M.S.S., Sarada, S., Kumar, C.G. and Prasad, R.B. (2017) Synthesis and in Vitro Antioxidant and Antimicrobial Studies of Novel Structured Phosphatidylcholines with Phenolic Acids. Food Chemistry, 221, 664-672. https://doi.org/10.1016/j.foodchem.2016.11.121
Toyigbénan, B.F., Raphiou, M., Marcellin, A., Durand, D.-N., Aklesso, N., Sylvestre, A., Haziz, S., Adolphe, A., Aly, S. and Lamine, B.-M. (2018) Ethnobotanical Survey of Three Species of Desmodium genus (Desmodium ramosissimum, Desmodium gangeticum and Desmodium adscendens) Used in Traditional Medicine, Benin. International Journal of Sciences, 7, 26-29. https://doi.org/10.18483/ijSci.1860
Attien, P., Sina, H., Moussaoui, W., et al. (2013) Prevalence and Antibiotic Resistance of Staphylococcus Strains Isolated from Meat Products Sold in Abidjan Streets (Ivory Coast). African Journal of Microbiology Research, 7, 3285-3293. https://doi.org/10.5897/AJMR2013.5688
Nanoukon, C., Argémi, X., Sogbo, F., Orekan, J, Keller, D., Affolabi, D., Schramm, F., Riegel, P., Baba-Moussa, L. and Prévost, P. (2017) Pathogenic Features of Clinically Significant Coagulase-Negative Staphylococci in Hospital and Community Infections in Benin. International Journal of Medical Microbiology, 307, 75-82. https://doi.org/10.1016/j.ijmm.2016.11.001
Dah-Nouvlessounon, D., Adoukonou-Sagbadja, H., Diarrassouba, N., Sina, H., Adjonohoun, A., Inoussa, M. and Baba-Moussa, L. (2015) Phytochemical Analysis and Biological Activities of Cola nitida Bark. Biochemistry Research International, 2015, Article ID: 493879. https://doi.org/10.1155/2015/493879
Sanogo, S., Sanogo, A.K. and Yossi, H. (2006) Collecte et conservation durable des graines et d’échantillons de plantes. Rapport de recherche de la campagne 2005/2006. 12e session de la Commission Scientifique de l’Institut d’économie Rurale (IER), Bamako.
N’Guessan, K., Kadja, B., Zirihi, G.N., Traoré, D. and Aké-Assi, L. (2009) Screening phytochimique de quelques plantes médicinales ivoiriennes utilisées en pays Krobou (Agboville, Cote-d’Ivoire). Sciences & Nature, 6, 1-15. https://doi.org/10.4314/scinat.v6i1.48575
Delarras, C. (1998) Microbiologie. 90 heures de travaux pratiques. Gaétan Morien Editeur. 169178.
Balouiri, M., Sadiki, M. and iIbnsouda, S.K. (2016) Methods for in Vitro Evaluating Antimicrobial Activity: A Review. Journal of Pharmaceutical Analysis, 6, 71-79. https://doi.org/10.1016/j.jpha.2015.11.005
Hosgor, L.M., Ermertcan, S., Erac, B. and Tasli, H. (2011) An Investigation of the Antimicrobial Impact of Drug Combinations against Mycobacterium tuberculosis Strains. Turkish Journal of Medical Sciences, 41, 719-724.
Kaya, O., Akcam, F. and Yayli, G. (2012) Investigation of the in Vitro Activities of Various Antibiotics against Brucella melitensis Strains. Turkish Journal of Medical Sciences, 42, 145-148.
Ouattara, L.H., Kabran, G.R.M., Guessennd, N.K., Konan, K.F., Mamyrbekova Bekro, J.A. and Bekro, Y.A. (2016) Antibacterial Activities in Vitro Extract from Root Bark of Mezoneuron benthamianum and Rods of Paullinia pinnata 2 Plants of the Pharmacopoeia Ivorian. Revue CAMES—Série Pharmacie Médecine Traditionnelle Africaine, 18, 31-40.
Jayashree, D. (2013) Phytochemicals and TLC Fingerprinting Analysis of Methanol Extracts of Three Medicinal Plants. International Journal of Pharmacy Research, 4, 123-126. https://doi.org/10.7897/2230-8407.04627
Jaradat, N.F. and Al Hussen Ali, A. (2015) Preliminary Phytochemical Screening, Quantitative Estimate of Total Flavonoids, Total Phenols and Antioxidant Activity of Ephedra alata Decne. Journal of Materials and Environmental Science, 6, 1771-1778.
Dohou, N., Yamni, K., Tahrouch, S., Hassani, L.M.I., Badoc, A. and Gmira, N. (2003) Screening of a Phytochemical Endemic Ibero-Moroccan Thymelaea Lythroides. Bulletin de la Société de Pharmacie, 142, 61-78.
Longanga, O., Vercruysse, A. and Foriers, A. (2000) Contribution to the Ethno Botanical, Phytochemical and Pharmacological Studies of Traditionally Used Medicinal Plants in the Treatment of Dysentery and Diarrhoea in Lomela Area, Democratic Republic of Congo (DRC). Journal of Ethnopharmacology, 71, 411-423. https://doi.org/10.1016/S0378-8741(00)00167-7
Mustapha, B.A., Kubmarawa, D., Shagal, M.H. and Ardo, B.P. (2016) Preliminary Phytochemical Screening of Medicinal Plants Found in the Vicinity of Quarry Site in Demsa, Adamawa State, Nigeria. American Chemical Science Journal, 11, 1-7. https://doi.org/10.9734/ACSJ/2016/21519
Ebana, R.U.B., Madunagu, B.E., Ekpe, E.D. and Otung, I.N. (1991) Microbiological Exploitation of Cardiac Glycoside and Alkaloids from Garcinia kola, Borreria ocymoides, Kola nitida and Citrus aurantifolia. Journal of Applied Biotechnology, 71, 398-401. https://doi.org/10.1111/j.1365-2672.1991.tb03807.x
Nguyi, A. (1988) Tannins of Some Nigerian Flora. Journal of Biotechnology, 6, 221-226.
Nsemi, M.F. (2010) Identification of Polyphenols, Evaluation of Their Antioxidant Activity and Study of Their Biological Properties. Doctoral Thesis, 295 p.
Nwachukwu, I.N., Allison, L.N., Chinakwe, E.C. and Nwadiaro, P. (2008) Studies on the Effects Cymbopogon citratus, Ceiba pentandra and Loranthus bengwelensis Extracts One Species of Dermatophytes. The Journal of American Science, 4, 58-67.
Sule, M.I., Njinga, N.S., Musa, A.M., Magaji, M.G. and Abdullah (2001) Antidiarrheal and Phytochemical Studies of the Stem Bark of Ceiba pentandra (Bombacaceae). Nigerian Newspaper of Pharmaceutical Sciences, 8, 143-148.
Attarde, D.L., Kadu, S.S., Chaudhari, B.J., Kale, S.S. and Bhamber, R.S. (2010) In Vitro Antioxidant Activity of Pericarp of Cucurbita maxima Duch. ex Lam. International Journal of PharmTech Research, 2, 1533-1538.
Consolacio, Y.R. and Kathlun, L. (2005) Sterols from Cucurbita maxima. Philippine Journal of Science, 134, 83-87.
Saha, P., Mazumder, U.K. and Haldar, P.K. (2011) In Vitro Antioxidant Activity of Cucurbita maxima Aerial Parts. Free Radicals and Antioxidants, 1, 42-48. https://doi.org/10.5530/ax.2011.1.8
Muruganantham, N., Solomon, S. and Senthamilselvi, M.M. (2016) Antimicrobial Activity of Cucurbita maxima Flowers (Pumpkin). Journal of Pharmacognosy and Phytochemistry, 5, 15-18.