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Composition and Molecular Weight Distribution of Albumin and Globulin Protein Isolates from Durum Wheat Genotypes
Department of Plant Science, Wollo University, Dessie, Ethiopia
Department of Plant Science, Wollo University, Dessie, Ethiopia
Department of Biology, Wollo University, Dessie, Ethiopia
Institute of Agricultural and Life Sciences, Jeonbuk National University, Jeonju, Republic of Korea
Department of Crop Science and Biotechnology, Jeonbuk National University, Jeonju, Republic of Korea
- 1 Department of Plant Science, Wollo University, Dessie, Ethiopia
- 2 Department of Plant Science, Wollo University, Dessie, Ethiopia
- 3 Department of Biology, Wollo University, Dessie, Ethiopia
- 4 Institute of Agricultural and Life Sciences, Jeonbuk National University, Jeonju, Republic of Korea
- 5 Department of Crop Science and Biotechnology, Jeonbuk National University, Jeonju, Republic of Korea
American Journal of Plant Sciences·Volume 11 (2020)·Pages 137–147·Published 22 January 2020·DOI10.4236/ajps.2020.112011
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Abstract
This paper attempts to evaluate the banding patterns of non-gluten protein isolates from the grain of durum wheat varieties. Under reduced condition, polyacrylamide-gel electrophoresis has revealed a number of different sized albumin and globulin protein bands. The electrophoretic pattern of globulin showed more polymorphisms than that of albumin. High polymorphism, both in band intensity and occurrence, was observed between 15 kDa and 35 kDa. Most of the protein bands were scored in the range of 10 kDa and 85 kDa in the two protein fractions. At a cutoff point 2.5, cluster analysis based on the SDS-PAGE of globulin proteins classified the durum wheat varieties into three major family groups. Generally, the experiment showed the suitability and usefulness of globulin protein fractions as a genetic marker in discriminating durum wheat genotypes.
KeywordsAlbuminDurum WheatGlobulinPolymorphismSeed Protein
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