Studies on Possible Modes of Action and Tolerance to Environmental Stress Conditions of Different Biocontrol Agents of Foliar Diseases in Maize — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Studies on Possible Modes of Action and Tolerance to Environmental Stress Conditions of Different Biocontrol Agents of Foliar Diseases in Maize
Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
,
Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
,
Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
,
Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
,
Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
,
Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
,
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
1 Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
2 Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
3 Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
4 Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
5 Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
6 Laboratorio de Ecología Microbiana Ambiental (ECOMA), Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Universidad Nacional de Río Cuarto, Córdoba, Argentina
7 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
The present study evaluates the possible modes of action of antagonistic bacteria and their tolerance to UV radiation, temperature and osmotic stress. The partial 16S-23S rRNA gene sequencing of eight antagonistic bacteria had a high match with three bacterial genera: Curtobacterium , Pantoea and Bacillus . In this study, the three Bacillus isolates showed the most relevant production of enzymes, volatile organic compounds and antibiosis against Exserohilum turcicum . Respect to UV radiation and temperature Pantoea and Bacillus isolates were more tolerant; whereas the eight isolates were tolerant to osmotic stress in varying degree. The three Bacillus isolates have the greatest potential as biocontrol agents for foliar diseases in maize. The antagonistic action could be explained through different modes of action such as enzymes, volatile organic compounds and/or direct antibiosis by other secondary metabolites. Bacillus isolates tolerance to environmental stresses including UV radiation, temperature and osmotic stress is relevant for survival and persistence on the leaf surface. This work provides new information about the mode of action of antagonistic bacteria with proven efficacy against maize leaf pathogens. In addition, it provides information about the tolerance of antagonistic bacteria against different stress conditions. The data of the present study could contribute to the development of a successful foliar biofungicide.
Bolsa de Cereales de Rosario (2018). http://www.bolsadecereales.com/imagenes/informes/2018-04/46-estimacionescdmaizar06-04-18.pdf
González, M. (2005) Roya común del maíz: altos niveles de severidad en la zona maicera núcleo. Rev. Agromensajes, No. 15. FCA-UNR. https://rephip.unr.edu.ar/bitstream/handle/2133/583/Roya%20com%25C3%25BAn%20del%20 ma%25C3%25ADz%20altos%20niveles%20de%20severidad%20en%20la%20zona%20maicera %20n%25C3%25BAcleo%20Campa%25C3%25B1a%2004-05.pdf?sequence=1
Rajeshwar, T., Reddy, P., Narayan, R., Ranga, R. and Reddy, S. (2013) Management of Turcicum Leaf Blight of Maize Caused by Exserohilum turcicum in Maize. International Journal of Science and Research Publications, 10, 2250-3153. http://www.ijsrp.org/research-paper-1013/ijsrp-p2290.pdf
Carmona, M. (2014) Umbrales para el control de las enfermedades foliares del maíz. [Thresholds for the Control of Foliar Diseases of Maize 2014]. http://www.agroconsultasonline.com.ar/documento.html
Carvalho, F.P. (2006) Agriculture Pesticides, Food Security and Food Safety. Environmental Science and Policy, 9, 685-692. https://doi.org/10.1016/j.envsci.2006.08.002
Alabouvette, C., Olivain, C. and Steinberg, C. (2006) Biological Control of Plant Diseases: The European Situation. European Journal of Plant Pathology, 114, 329-341. https://doi.org/10.1007/s10658-005-0233-0
Castoria, R. and Wright, S. (2009) Host Responses to Biological Control Agents. In: Prusky, D. and Gullino, M. Eds., Post-Harvest Pathology, Vol. 2, Springer, Amsterdam, 171-181. https://doi.org/10.1007/978-1-4020-8930-5_12
Legrand, F., Picot, A., Cobo-Díaz, J.F., Chen, W. and Le Floch, G. (2017) Challenges Facing the Biological Control Strategies for the Management of Fusarium Head Blight of Cereals Caused by F. graminearum. Biological Control, 113, 26-38. https://doi.org/10.1016/j.biocontrol.2017.06.011
Sartori, M., Nesci, A., Formento, A. and Etcheverry, M. (2015) Selection of Potential Biological Control of Exserohilum turcicum with Epiphytic Microorganisms from Maize. Revista Argentina de Microbiología, 47, 62-71. https://doi.org/10.1016/j.ram.2015.01.002
Sartori, M., Nesci, A., Garcia, J., Passone, M.A., Montemarani, A. and Etcheverry, M. (2017) Efficacy of Epiphytic Bacteria to Prevent Northern Leaf Blight Caused by Exserohilum turcicum in Maize. Revista Argentina de Microbiología, 49, 75-82. https://doi.org/10.1016/j.ram.2016.09.008
Sartori, M., Nesci, A., Montemarani, A., Barros, G., Garcia, J. and Etcheverry, M. (2017) Preliminary Evaluation of Biocontrol Agents against Maize Pathogens Exserohilum turcicum and Puccinia sorghi in Field Assays. Agricultural Sciences, 8, 1003-1013. https://doi.org/10.4236/as.2017.89073
Lindow, S. and Brandl, M. (2003) Microbiology of the Phyllosphere. Applied and Environmental Microbiology, 69, 1875-1883. https://aem.asm.org/content/aem/69/4/1875.full.pdf https://doi.org/10.1128/AEM.69.4.1875-1883.2003
Beattie, G. and Lindow, S. (1995) The Secret Life of Foliar Bacterial Pathogens on Leaves. Annual Review of Phytopathology, 33, 145-172. https://doi.org/10.1146/annurev.py.33.090195.001045
Hall, T. (1999) BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Zhang, Z., Schwartz, S., Wagner, L. and Miller, W. (2000) A Greedy Algorithm for Aligning DNA Sequences. Journal of Computacional Biology, 7, 203-214. https://doi.org/10.1089/10665270050081478
Atlas, R.M. (2005) Handbook of Media for Environmental Microbiology. 2nd Edition, CRC Press, Boca Raton.
Khabbaz, S., Zhang, L., Cáceres, L., Sumarah, M., Wang, A. and Abbasi, P. (2015) Characterisation of Antagonistic Bacillus and Pseudomonas Strains for Biocontrol Potential and Suppression of Damping-Off and Root Rot Diseases. Annals of Applied Biology, 166, 456-471. https://doi.org/10.1111/aab.12196
Zheng, Y. and Wozniak, C.A. (1997) Adaptation of a Beta-1, 3-Glucanase Assay to Microplate Format. Biotechniques, 22, 922-926. https://doi.org/10.2144/97225st06
Bautista-Rosales, P.U., Calderon-Santoyo, M., Servín-Villegas, R., Ochoa-álvarez, N.A., Vázquez-Juárez, R. and Ragazzo-Sánchez, J.A. (2014) Biocontrol Action Mechanisms of Cryptococcus laurentii on Colletotrichum gloeosporioides of Mango. Crop Protection, 65, 194-201. https://doi.org/10.1016/j.cropro.2014.07.019
Gascón, J., Oubiña, A., Pérez-Lezaun, A. and Urmeneta, J. (1995) Sensitivity of Selected Bacterial Species to UV Radiation. Current Microbiology, 30, 177-182. https://doi.org/10.1007/BF00296205
Sartori, M., Nesci, A. and Etcheverry, M. (2010) Impact of Osmotic/Matric Stress and Heat Shock on Environmental Tolerance Induction of Bacterial Biocontrol Agents against Fusarium verticillioides. Research in Microbiology, 161, 681-686. https://doi.org/10.1016/j.resmic.2010.06.008
Dallyn, H. and Fox, A. (1980) Spoilage of Material of Reduced Water Activity by Xerophilic Fungi. In: Gould, G. and Corry, E., Eds., Society of Applied Bacteriology Technical Series 15, Academic Press, London, 219-239.
Di Rienzo, J., Casanoves, F., Balzarini, M., González, L., Tablada, M. and Robledo, C. (2013) InfoStat, versión 2013, Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina.
Kai, M., Effmert, U., Berg, G. and Piechulla, B. (2007) Volatiles of Bacterial Antagonists Inhibits Mycelial Growth of the Plant Pathogen Rhizoctonia solani. Archives of Microbiology, 187, 351-360. https://doi.org/10.1007/s00203-006-0199-0
Hirakawa, H., Inazumi, Y., Masaki, T., Hirata, T. and Yamaguchi, A. (2005) Indole Induces the Expression of Multidrug Exporter Genes in Escherichia coli. Molecular Microbiology, 55, 1113-1126. https://doi.org/10.1111/j.1365-2958.2004.04449.x
Mueller, R.S., Beyhan, S., Saini, S.G., Yildiz, F.H. and Bartlett, D.H. (2009) Indole Acts as an Extracellular Cue Regulating Gene Expression in Vibrio cholerae. Journal of Bacteriology, 191, 3504-3516. https://doi.org/10.1128/JB.01240-08
Bruce, A., Stewart, D., Verrall, S. and Wheatley, R. (2003) Effect of Volatiles from Bacteria and Yeast on the Growth and Pigmentation of Sapstain Fungi. International Biodeterioration and Biodegradation, 51, 101-108. https://doi.org/10.1016/S0964-8305(02)00088-4
Yuan, J., Raza, W., Shen, Q. and Huang, Q. (2012) Antifungal Activity of Bacillus amyloliquefaciens NJN-6 Volatile Compounds against Fusarium oxysporum f. sp. cubense. Applied and Environmental Microbiology, 78, 5942-5944. https://doi.org/10.1128/AEM.01357-12
Wu, Y., Yuan, J., Raza, W., Shen, Q. and Huang, Q. (2015) Effects of Volatile Organic Compounds from Streptomyces albulus NJZJSA2 on Growth of Two Fungal Pathogens. Journal of Basic Microbiology, 55, 1104-1117. https://doi.org/10.1002/jobm.201400906
Elad, Y. (1995) Mycoparasitism. In: Kohmoto, K., Singh, U.S. and Singh, R.P., Eds., Pathogenesis and Host Specicity in Plant Diseases: Histopathological, Biochemical, Genetic and Molecular Basis, Eukaryotes, Vol. II, Elsevier, Oxford Pergamon, 289-307.
Duffy, B. (2003) Pathogen Self-Defense: Mechanisms to Counteract Microbial Antagonism. Annual Review of Phytopathology, 41, 501-538. https://doi.org/10.1146/annurev.phyto.41.052002.095606
Chen, X.H., Koumoutsi, A., Scholz, R., Schneider, K., Vater, J. and Sussmuth, R. (2009) Genome Analysis of Bacillus amyloliquefaciens FZB42 Reveals Its Potential for Biocontrol of Plant Pathogens. Journal of Biotechnology, 140, 27-37. https://doi.org/10.1016/j.jbiotec.2008.10.011
Dunlap, C.A., Bowman, M.J. and Schisler, D.A. (2013) Genomic Analysis and Secondary Metabolite Production in Bacillus amyloliquefaciens AS 43.3: A Biocontrol Antagonist of Fusarium Head Blight. Biological Control, 64, 166-175. https://doi.org/10.1016/j.biocontrol.2012.11.002
Palazzini, J.M., Dunlap, C.A., Bowman, M.J. and Chulze, S.N. (2016) Bacillus velezensis RC 218 as a Biocontrol Agent to Reduce Fusarium Head Blight and Deoxynivalenol Accumulation: Genome Sequencing and Secondary Metabolite Cluster Profiles. Microbiological Research, 192, 30-36. https://doi.org/10.1016/j.micres.2016.06.002