Effect of Molasses Addition at Ensiling on Ruminal <i>In Situ</i> Dry Matter and Nutrient Degradation of Whole-Plant Soybean Silage Harvested at Different Phenological Stages — Oak Academic Publishing
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Effect of Molasses Addition at Ensiling on Ruminal <i>In Situ</i> Dry Matter and Nutrient Degradation of Whole-Plant Soybean Silage Harvested at Different Phenological Stages
Department of Animal Nutrition and Animal Production, University of Sao Paulo, Pirassununga, Brazil
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Department of Animal and Dairy Sciences, University of Wisconsin, Madison, USA
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Department of Animal and Dairy Sciences, University of Wisconsin, Madison, USA
,
Federal University of Santa Maria, Santa Maria, Brazil
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Department of Animal Nutrition and Animal Production, University of Sao Paulo, Pirassununga, Brazil
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Department of Animal and Dairy Sciences, University of Wisconsin, Madison, USA
1 Department of Animal Nutrition and Animal Production, University of Sao Paulo, Pirassununga, Brazil
2 Department of Animal and Dairy Sciences, University of Wisconsin, Madison, USA
3 Department of Animal and Dairy Sciences, University of Wisconsin, Madison, USA
4 Federal University of Santa Maria, Santa Maria, Brazil
5 Department of Animal Nutrition and Animal Production, University of Sao Paulo, Pirassununga, Brazil
6 Department of Animal and Dairy Sciences, University of Wisconsin, Madison, USA
Whole-plant soybean ensiling has limitations and challenges that affect silage fermentation patterns and reduce ruminal nutrient degradation. Perhaps either the addition of molasses at ensiling or harvesting at different phenological stages has the potential to enhance whole-plant soybean silage (WSS) ruminal degradation. This experiment was a completely randomized design with a 3 × 2 factorial arrangement of treatments evaluating the effects of molasses (0 and 40 g/kg fresh forage) and phenological stage (R5: beginning seed, R6: full seed, and R7: beginning maturity) on ruminal in situ degradation. Molasses increased effective ruminal degradability (ERD) of dry matter (DM) regardless of the phenological stage and increased ERD of crude protein at R5 and R6. The addition of molasses at later phenological stages increased ERD of neutral detergent fiber (NDF). There was a molasses × phenological stage interaction effect with greater fractions A and B of NDF at R7 and R5, respectively, but lower undegraded NDF at R5 and R6 with molasses addition. Although the benefits of adding molasses were more pronounced in R5 and R6, adding molasses to whole-plant soybean at ensiling is recommended regardless of phenological stage. Increasing the proportion of digestible nutrients at harvesting either by harvesting at later phenological stages or the addition of molasses is a powerful strategy to manage ruminal degradation of DM and nutrients in WSS.
Baghdadi, A., Halim, R.A., Radziah, O., Martini, M.Y. and Ebrahimi, M. (2016) Fermentative Characteristics and Nutritive Value of Corn Silage Intercropped with Soybean under Different Crop Combination Ratios. The Journal of Animal & Plant Sciences, 26, 1710-1717.
Parra, C.S., Bolson, D.C., Jacovaci, F.A., Nussio, L.G., Jobim, C.C. and Daniel, J.L.P. (2019) Influence of Soybean-Crop Proportion on the Conservation of Maize-Soybean Bi-Crop Silage. Animal Feed Science and Technology, 257, Article ID: 114295. https://doi.org/10.1016/j.anifeedsci.2019.114295
Ghizzi, L.G., Del Valle, T.A., Zilio, E.M.C., Sakamoto, L.Y., Marques, J.A., Dias, M.S.S., Nunes, A.T., Gheller, L.S., Silva, T.B.P., Grigoletto, N.T.S., Takiya, C.S., Silva, G.G. and Rennó, F.P. (2020) Partial Replacement of Corn Silage with Soybean Silage on Nutrient Digestibility, Ruminal Fermentation, and Milk Fatty Acid Profile on Dairy Cows. Animal Feed Science and Technology, 266, Article ID: 114526. https://doi.org/10.1016/j.anifeedsci.2020.114526
Ni, K., Wang, F., Zhu, B., Yang, J., Zhou, G., Pan, Y., Tao, Y. and Zhong, J. (2017) Effects of Lactic Acid Bacteria and Molasses Additives on the Microbial Community and Fermentation Quality of Soybean Silage. Bioresource Technolonogy, 238, 706-715. https://doi.org/10.1016/j.biortech.2017.04.055
McDonald, P., Henderson, N. and Herson, S. (1991) The Biochemistry of Silage. 2nd Edition, Chalcombe, Marlow.
Tobía, C., Villalobos, E., Rojas, A., Soto, H. and Moore, K.J. (2008) Nutritional Value of Soybean (Glycine max L. Merr.) Silage Fermented with Molasses and Inoculated with Lactbacillus brevis 3. Livestock Research for Rural Development, 20, Article No. 106.
Rigueira, J.P.S., Pereira, O.G., Valadares Filho, S.C., Ribeiro, K.G., Garcia, R. and Cezáreo, A.S. (2015) Soybean Silage in the Diet for Beef Cattle. Acta Scientiarum, 37, 61-65. https://doi.org/10.4025/actascianimsci.v37i1.25182
Spanghero, M., Zanfi, C., Signor, M., Davanzo, D., Volpe, V. and Venerus, S. (2015) Effects of Plant Vegetative Stage and Field Drying Time on Chemical Composition and In Vitro Ruminal Degradation of Forage Soybean Silage. Animal Feed Science and Technology, 200,102-106. https://doi.org/10.1016/j.anifeedsci.2014.11.006
Kawamoto, H., Touno, E., Uchino, H. and Uozumi, S. (2013) Comparison of Fermentation Quality and Ruminal Degradability between Two Different Harvest Timings of Forage Soybean (Glycine max (L.) Merr.) Ensiled with the Corn-Silage System. Grassland Science, 59, 120-123. https://doi.org/10.1111/grs.12015
Dias, J.F., Jobim, C.C., Filho, J.L.S., Junior, V.H.B., Poppi, E.C. and Santello, G.A. (2010) Chemical Composition and Dry Matter Total Losses of Soybean Plant Silage. Acta Scientiarum. Animal Sciences, 32, 19-26.
Ghizzi, L.G. (2020) Whole-Plant Soybean Silage Management and Its Use in Dairy Cow’s Diet. PhD Thesis, University of São Paulo, Pirassununga.
Fehr, W.R. and Caviness, C.E. (1977) Stage of Soybean Development. Special Report No. 80. Co-Operative Extension Service, Iowa State University, Ames.
AOAC International (2012) Official Methods of Analysis. AOAC International, 19th Edition, AOAC International, Arlington.
AOAC International (2000) Official Methods of Analysis. AOAC International, 17th Edition, AOAC International, Arlington.
Van Soest, P.J., Robertson, J.B. and Lewis, B.A. (1991) Methods for Dietary Fiber, Neutral Detergent Fiber, Non-Starch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74, 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Undersander, D., Mertens, D.R. and Thiex, N. (1993) Forage Analyses Procedures. National Forage Testing Association, Omaha.
Hall, M.B. (2000) Calculation of Non-Structural Carbohydrate Content of Feeds That Contain Non-Protein Nitrogen. Bulletin No. 339, University of Florida, Gainesville, A-25.
Playne, M.J. and McDonald, P. (1966) The Buffering Constituents of Herbage and of Silage. Journal of the Science Food and Agriculture 17, 264-268. https://doi.org/10.1002/jsfa.2740170609
Broderick, G.A. and Kang, J.H. (1980) Automated Simultaneous Determination of Ammonia and Total Amino Acids in Ruminal Fluid in Vitro Media. Journal of Dairy Science, 63, 64-75. https://doi.org/10.3168/jds.S0022-0302(80)82888-8
Pryce, J.D.A. (1969) Modification of the Barker-Summerson Method for the Determination of Latic Acid. Analyst, 94, 1151-1152. https://doi.org/10.1039/an9699401151
Ørskov, E.R. and McDonald, I. (1979) The Estimation of Protein Degradability in the Rumen from Incubation Measurements Weighted According to Rate of Passage. The Journal of Agricultural Science. 92, 499-503. https://doi.org/10.1017/S0021859600063048
Kenward, M.G. and Roger, J.H. (1997) Small Sample Inference for Fixed Effects from Restricted Maximum Likelihood. Biometrics, 53, 983-997. https://doi.org/10.2307/2533558
Zhai, G., Shen, Y., Zhai, Y., Liu, X. and Jiag, H. (2013) Forage Yield Performance and Nutritive Value of Selected Wild Soybean Ecotypes. Canadian Journal of Plant Science, 88, 465-472. https://doi.org/10.4141/CJPS06005
Sniffen, C.J., O’connor, J.D., Van Soest, P.J., Fox, D.G. and Russel, J.B. (1992) A Net Carbohydrate and Protein System for Evaluating Cattle Diets: II. Carbohydrate and Protein Availability. Journal of Animal Science, 70, 3562-3577. https://doi.org/10.2527/1992.70113562x
Varga, G.A. and Hoover, W.H. (1983) Rate and Extent of Neutral Detergent Fiber Degradation of Feedstuffs In Situ. Journal of Dairy Science, 66, 2109-2115. https://doi.org/10.3168/jds.S0022-0302(83)82057-8
Bruno-Soares, A.M., Abreu, J.M.F., Guedes, C.V.M. and Dias-da-Silva, A.A. (2000) Chemical Composition, DM and NDF Degradation Kinetics in Rumen of Seven Legume Straws. Animal Feed Science and Technology, 83, 75-80. https://doi.org/10.1016/S0377-8401(99)00113-3
Kung Jr, L., Shaver, R.D., Grant, R.J. and Schmidt, R.J. (2018) Silage Review: Interpretation of Chemical, Microbial, and Organoleptic Components of Silages. Journal of Dairy Science, 101, 4020-4033. https://doi.org/10.3168/jds.2017-13909
Russell, J.B., O’Connor, J.D., Fox, D.G., Van Soest, P.J. and Sniffen, C.J. (1992) A Net Carbohydrate and Protein System for Evaluating Cattle Diets: I. Ruminal Fermentation. Journal of Animal Science, 70, 3551-3561. https://doi.org/10.2527/1992.70113551x
National Research Council (2001) Nutrient Requirements of Dairy Cattle. 7th Revised Edition, National Academies Press, Washington DC.
Higgs, R.J., Chase, L.E., Ross, D.A. and Van Amburgh, M.E. (2015) Updating the CNCPS Feed Library and Analyzing Model Sensitivity to Feed Inputs. Journal of Dairy Science, 98, 6340-6360. https://doi.org/10.3168/jds.2015-9379
Janicki, F.J. and Stallings, C.C. (1988) Degradation of Crude Protein in forages Determined by In Vitro and In Situ Procedures. Journal of Dairy Science, 71, 2440-2448. https://doi.org/10.3168/jds.S0022-0302(88)79829-X
Mertens, D.R. (2016) Measuring and Using uNDF to Improve Dairy Nutrition. Southwest Nutrition Conference, Tempe, 17-19 February 2016, 12-19.
Wilson, J.R. and Kennedy, P.M. (1996) Plant and Animal Constraints to Voluntary Feed Intake Associated with Fibre Characteristics and Particle Breakdown and Passage in Ruminants. Australian Journal of Agricultural Research, 47, 199-225. https://doi.org/10.1071/AR9960199
Jung, H.G. and Allen, M.S. (1995) Characteristics of Plant Cell Walls Affecting Intake and Digestibility of Forages by Ruminants. Journal of Animal Science, 73, 2774-2790. https://doi.org/10.2527/1995.7392774x