In this research, soil microbial structures under a wheat triennial monoculture and horse bean-wheat-horse bean succession were evidenced using a metagenomic approach. Polymorphism analysis of DNA extracted from soil samples collected at the end of the third year of the two crop successions, was performed by PCR, carried-out with six different primers designed on simple tandem repeats sequences. Readable profiles were obtained with M13 primer, from which no polymorphisms were detected, and with the primer (GACA) 4 , that gave distinctive patterns. Experimental findings suggest that metagenomic analysis performed by (GACA) 4 primer may be an easy and suitable method to discriminate microbial diversity of different crop successions. (GACA) 4 PCR-pattern indicate that soil microbiota changes are well correlated with crop succession.
KeywordsCover PlantsHot-Spot VariabilityMini- and Micro-satellitePCR FingerprintingSoil Biodiversity
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