Honey bees and bee products are optimal monitors of the environmental quality; experimental beehives were placed in strategic places of Gran Sasso-Monti della Laga Park. Pollen and honey, produced in this protected area, were analyzed in order to demonstrate their good quality respect to those commercials. Physico-chemical parameters (water content, hydroxymethylfurfural), total flavonoid and phenolic contents and residue analysis (heavy metals, pesticides, tetracycline, sulfathiazole) were carried and evidenced their high quality. Moreover, melissopalynological analysis allowed us to establish a floristic census within the different places of the park. Data show that bee products are good quality, contain high levels of phenolics and flavonoids and show absence of pesticides and low concentration level of heavy metals and antibiotics. Due to this the human impact, even in protected areas, cannot be neglected; similarly environmental contamination by wild livestock was seen in different monitored sites.
Crane, E. (1984) Bees, honey and pollen as indicators of metals in the environment. Bee World, 55, 47-49
Ricciardelli, d’Albore, G. and Intoppa, F. (2000) Apoidei e Ambiente. In: Ricciardelli d’Albore, G. and Intoppa, F., Eds., Fiori e Api. La flora visitata dalle Api e dagli altri Apoidei in Europa. Calderini Edagricole, Bologna, 12-15.
Bogdanov, S. (2006) Contaminants of bee products. Apidologie, 37, 1-18. doi:10.1051/apido:2005043
Celli, G. (1983) L’ape come insetto test della salute di un territorio. In: Arzone, A., Conti, M., Currado, I., Marletto, F., Pagiano, G., Ugolini, A. and Vidano, C. Eds., Atti XIII Congresso Nazionale Italiano Entomologia, Torino, 637- 644.
Porrini, C., Ghini, S., Girotti, S., Sabatini, A.G., Gattavecchia, E. and Celli, G. (2002) Use of honey bees as bioindicators of environmental pollution in Italy. In: Dellivers, J. and Pham-Delègue, M. Eds., Honey Bees: Estimating the environment Impact of Chemicals, Taylor & Francis, London, 186-247.
Porrini, C., Sabatini, A.G., Girotti, S., Ghini, S., Medrzycki, P., Grillenzoni, F., Bortolotti, L., Gattavecchia, E. and Celli, G. (2003) Honey bees and bee products as monitors of the environmental contamination. Apiacta, 38, 63-70.
Hoffmann, F. (2005) Biodiversity and pollination: Flowering plants and flore-visiting insects in agricultural and semi-natural landscape [dissertation]. University of Groningen. Accessed 2007 Oct 15. http://dissertations.ub.rug.nl/faculties/science/2005/f.hoffmann/
National Research Council. (2007) Status of Pollinators in North America. The National Academies Press, Washington DC.
Winfree, R. (2010) The conservation and restoration of wild bees. Annals of the New York Academy of Sciences, 1195, 169-197. doi:10.1111/j.1749-6632.2010.05449.x
Repubblica Italiana. (2004) Decreto legislativo N.179 del 21 maggio 2004, attuazione della direttiva 2001/110/CE concernente il miele. Gazzetta Ufficiale del 20/7/2004 n. 168.
UNI 10934, (2001) Miele determinazione dell’idrossi-metilfurfurale. Ente Nazionale Italiano di Unificazione.
Von Der Ohe, W., Persano Oddo, L., Piana, M.L., Merlot, M. and Martin, P. (2004) Harmonized methods of melissopalynology. Apidologie, 35, 18-25. doi:10.1051/apido:2004050
Heimler, D., Vignolini, P., Dini, M.G. and Romani, A. (2005) Rapid tests to assess the antioxidant activity of Phaseolus vulgaris L. Dry beans. Journal of Agricultural and Food Chemistry, 53, 3053-3056. doi:10.1021/jf049001r
Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. (2004) Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91, 571-577. doi:10.1016/j.foodchem.2004.10.006
Bradford, M.M. (1976) Rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of proteindye binding. Analytical Bio-chemistry, 72, 248-254. doi:10.1016/0003-2697(76)90527-3
Huq, S., Garriques, M. and Kallury, K.M.R. (2006) Role of zwitterionic structures in thesolid-phase extraction based method development for clean up of tetracycline and oxytetracycline from honey. Journal of Chromatography A, 1135, 12-18. doi:10.1016/j.chroma.2006.09.025
Oka, H., Ito, Y., Ikai, Y., Kagami, T. and Harada, K. (1998) Mass spectrometric analysis of tetracycline antibiotics in food. Journal of Chromatography, 812, 309-319. doi:10.1016/S0021-9673(97)01278-8
Alfredsson, G., Branzell, C., Granelli, K. and Lundstr?m, ?. (2005) Simple and rapid screening and confirmation of tetetracycline in honey and eggs by a dipstick test and LC-MS/MS. Analytica Chimica Acta, 529, 47-51. doi:10.1016/j.aca.2004.08.050
Martel, A.C. and Zeggane, S. (2003) HPLC determination of sulfathiazole in French honeys. Journal of Liquid Chromatography & Related Technologies, 26, 953-961. doi:10.1081/JLC-120018895
Martel, A.C. and Zeggane, S. (2002) Determination of acaricides in honey by high-performance liquid chromatography with photodiode array detection. Journal of Chromatography A, 954, 173-180. doi:10.1016/S0021-9673(02)00126-7
Perkin Elmer Corporation. (1982) Analytical methods for Atomic Absorption Spectrophotometer, Manuel, Fish Products, 4-5.
AOAC. (1984) Official methods of analysis of the Association of Official Analytical Chemists. 14th Edition, Association of Official Analytical Chemists, Washington DC.
Celli, G. (1994) L’ape come indicatore biologico dei pesticidi. In: D’Ambrosio, M. T. and Accorti, M., Eds., Atti del convegno: “L’Ape come insetto test dell’inquinamento agricolo” P.F “Lotta biologica e integrata per la diffusione delle colture agrarie e delle piante forestali” March 28, 1992, Florence, Italy. Ministero Agricoltura e Foreste, Rome, 15-20.
Porrini, C., Sabatini, A.G., Girotti, S., Ghini, S., Medrzycki, P., Grillenzoni, F., Bortolotti, L., Gattavecchia, E. and Celli, G. (2003) Honey bees and bee products as monitors of the environmental contamination. Apiacta, 38, 63-70.
Persano Oddo, L. (2000) La qualità e la valorizzazione del miele. In: Persano Oddo, L., Sabatini, A.G., Accorti, M., Colombo, R., Marcazzan, G.L., Piana, M.L., Piazza, M.G. and Pulcini, P., Eds., I Mieli Uniflorali Italiani. Nuove schede di caratterizzazione, 105.
Repubblica Italiana, (2004) G. U. No. 168 del 20/07/2004. Decreto legislativo 21 maggio 2004. Attuazione della direttiva 2001/110/CEE concernente la produzione e commercializzazione del miele. Italy.
Conti, F. and Manzi, A. (1997) Centaurea diluita Aiton, new to the Italian flora. Flora Mediterranea, 7, 51-53.
Conti, F., Manzi, A. and Tinti, D. (2002) Aggiunte alla flora d’Abruzzo. Informatore Botanico Italiano, 34, 55- 61.
Conti, F. (2004) La flora ipsofila dell’Appennino centrale: ricchezza ed endemiti. Informatore Botanico Italiano, 35, 383-386.
Conti, F., Tinti, D., Scassellatti, E., Bartolucci, F. and Di Santo, D. (2007) Le piante vascolari endemiche dell’ Appennino centrale. Biogeographia, XXVIII, 25-38.
Bentrocelj, J., Dober?ek, U., Jamnik, M. and Golob, T., (2007) Evaluation of the phenolic content, antioxidant activity and color of Slovenian honey. Food Chemistry, 105, 822-828. doi:10.1016/j.foodchem.2007.01.060
Pichichero, E., Canuti, L. and Canini, A. (2009) Characterisation of the phenolic and flavonoid fractions and antioxidant power of italian honeys of different botanical origin. Journal of the Science of Food and Agriculture, 89, 609-616. doi:10.1002/jsfa.3484
Lila, M.A. (2006) Perspective: The nature-versus-nurture debite on bioactive phytochemicals: The genome versus terrori. Journal of the Science of Food and Agriculture, 48, 1498-1150.
Canini, A., Pichichero, E., Alesiani, D., Canuti, L. and Leonardi, D. (2009) Nutritional and botanical interest of honey collected from protected natural areas. Plant Biosystems, 143, 62-70. doi:10.1080/11263500802633543
Schramm, D.D., Karim, M., Schrader, H.R., Holt, R.R., Cardetti, M. and Keen, C.L. (2003) Honey with high levels of antioxidants can provide protection to healthy human subjects. Journal of the Science of Food and Agriculture, 51, 1732-1735. doi:10.1021/jf025928k
Gheldof, N., Wang, X.H. and Engheseth, N.J. (2002) Identification and qualification of antioxidant components of honey from various floral sources. Journal of Agricultural and Food Chemistry, 50, 5870-5877. doi:10.1021/jf0256135
McKibben, J. and Engheseth, N.J. (2002) Honey as a protective agent against lipid oxidation in ground turkej. Journal of Agricultural and Food Chemistry, 50, 592-595. doi:10.1021/jf010820a
Alesiani, D., Cicconi, R., Mattei, M., Montesano, C., Bei, R. and Canini, A. (2008) Cell cycle arrest and differentiation induction by 5,7-dimethoxycoumarin in melanoma cell lines. International Journal of Oncology, 32, 425-434.
Migliore, L., Godeas, F., De Filippis, S.P., Mantovi, P., Barchi, D., Testa, C., Rubattu, N. and Brambilla, G. (2010) Hormetic effect(s) of tetracycline as environmental contaminant on Zea Mays, Environment and Pollution, 158, 129-134. doi:10.1016/j.envpol.2009.07.039
Kumar, K., Gupta, S.C., Baidoo, S.K., Chander, Y. and Singh, A.K. (2005) Antibiotic use in agricolture and its impact on the terrestrial environment. Advances in Agronomy, 87, 1-54. doi:10.1016/S0065-2113(05)87001-4
Dolliver, H., Kumar, K. and Gupta, S. (2007) Sulphamethazine Uptake by Plants from Manure-Amended Soil. Journal of Environmental Quality, 36, 1224-1230. doi:10.2134/jeq2006.0266
Bogdanov, S., Imdorf, A., Charrière, J.D., Fluri, P. and Kilchenmann, V. (2001) Quality of bee products and sources of contamination. LApis, 11, 5-10.
Sungpyo, K. and Diana, S. A. (2007) Potential ecological and human health impacts of antibiotics and antibiotic-resistant bacteria from wastewater treatment plants. Journal of Toxicology and Environmental Health, Part B, 10, 559-573.
Halling-Sorensen, B., Nors Nielsen, S.N., Lanzky, P.F., et al., (1998) Occurrence, Fate and Effects of Pharmaceutical Substances in the Environment—A Review. Chemosphere, 36, 357-393.
Debayle, D., Dessalces, G. and Granier-Loustalot, M.F. (2008) Multi-residue analysis of traces of pesticides and antibiotics in honey by HPLC-MS-MS. Analytical and Bioanalytical Chemistry, 391, 1011-1020. doi:10.1007/s00216-008-2003-2
National Residues Plan. (2010) ai sensi dell’ art. 13 del D.L. del 16 marzo 2006 n. 158 per la ricerca di residui negli animali e nei prodotti di origine animale.