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Isolation and expression analysis of CBF4 from <i>Vitis amurensis</i> associated with stress
College of Horticulture, Nanjing Agricultural University, Nanjing, China
College of Horticulture, Nanjing Agricultural University, Nanjing, China
College of Horticulture, Nanjing Agricultural University, Nanjing, China
College of Horticulture, Nanjing Agricultural University, Nanjing, China
Department of Horticulture, Heilongjiang Academy of Agricultural Science, Harbin, China
Department of Horticulture, Heilongjiang Academy of Agricultural Science, Harbin, China
Department of Horticulture, Heilongjiang Academy of Agricultural Science, Harbin, China
College of Horticulture, Nanjing Agricultural University, Nanjing, China
College of Horticulture, Nanjing Agricultural University, Nanjing, China
- 1 College of Horticulture, Nanjing Agricultural University, Nanjing, China
- 2 College of Horticulture, Nanjing Agricultural University, Nanjing, China
- 3 College of Horticulture, Nanjing Agricultural University, Nanjing, China
- 4 College of Horticulture, Nanjing Agricultural University, Nanjing, China
- 5 Department of Horticulture, Heilongjiang Academy of Agricultural Science, Harbin, China
- 6 Department of Horticulture, Heilongjiang Academy of Agricultural Science, Harbin, China
- 7 Department of Horticulture, Heilongjiang Academy of Agricultural Science, Harbin, China
- 8 College of Horticulture, Nanjing Agricultural University, Nanjing, China
- 9 College of Horticulture, Nanjing Agricultural University, Nanjing, China
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Abstract
The C-repeat Binding Factor/Dehydration Responsive Element Binding factor (CBF/DREB) r esponsive pathway is involved in plant re sponse to abiotic stress. In this study, we report the isolation of VaCBF4 (a complete cDNA) and its promoter from Vitis amurensis through rapid amplification of cDNA ends and genome walking t echniques, respectively. The CBF4 transcrip t accumulation of V. amurensis increased under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA) treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent manner. The presence of the cis -elements MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this promoter is a component of the CBF transduction pathway, which is involved in plant response to multistress.
Keywords<i>Vitis amurensis</i>CBF4StressTranscript Accumulation
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