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Comparative Study of Bunt Pathogen Resistance to the Effects of Fungicides in Callus Co-Cultures <i>Triticum aestivum</i> with Tilletia caries
Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia
Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia
Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia
Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia
Bashkir State University, Ufa, Russia
Bashkir State University, Ufa, Russia
- 1 Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia
- 2 Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia
- 3 Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia
- 4 Institute of Biochemistry and Genetics of Ufa Scientific Center of Russian Academy of Sciences, Ufa, Russia
- 5 Bashkir State University, Ufa, Russia
- 6 Bashkir State University, Ufa, Russia
Agricultural Sciences·Volume 05 (2014)·Pages 906–912·Published 7 August 2014·DOI10.4236/as.2014.510098
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Abstract
The morphophysiological and molecular-genetic parameters of T. caries isolates collected from various fields of Southern Urals agrocenoses have been analysed. Isolate 1 of wheat callus had a high growth rate in vitro even in the presence of 0.1 mg/l fungicide Baitan. Isolate 2 of wheat callus had a low growth rate whereas 0.1 mg/l Baitan significantly inhibited its growth. Comparison of nucleotide sequences of 18S RNA gene of the two isolates showed high level of homology between them, but a large number of nucleotide substitutions have been found. The most characteristic excision was five nucleotides at position 461 of the isolate 2, compared with the isolate 1. Our results allow to assume that the environmental stress including high pesticide concentration may cause the resistance of T. caries pathogen to fungicides.
KeywordsWheat<i>Tilletia caries</i>CalliTriadimenol18S RNAResistance to Fungicides
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