Effects of autoclaving on the physiological action of paclobutrazol
- 1 Depto de Biologia Vegetal/Universidade Federal de Vi?osa, Vi?osa, Brazil;
- 2 Depto de Biologia Vegetal/Universidade Federal de Vi?osa, Vi?osa, Brazil;
- 3 Depto de Biologia Vegetal/Universidade Federal de Vi?osa, Vi?osa, Brazil;
- 4 Depto de Biologia Vegetal/Universidade Federal de Vi?osa, Vi?osa, Brazil;
- 5 Depto de Biologia Vegetal/Universidade Federal de Vi?osa/36570-000 Vi?osa, MG, Brazil
Abstract
Besides being employed as an efficient plant growth retardant for field, garden and potted plants, paclobutrazol (PBZ) is also used in laboratory experiments, mainly in aseptic cultures, both in autoclaved and non-autoclaved form. Therefore it is not known if autoclaving can partial or completely inactivates the product, thus decreasing its efficacy. Thus a simple experiment was carried out to assess to what extent the autoclaving process can affect some growth components and dry mass accumulation and partition in sunflower seedlings, by employing the techniques of plant Growth Analysis. Autoclaving did not modify either qualitative or quantitatively any of the plant responses to PBZ as well their reversions by gibberellic acid.
- PGRSA (2007) Plant growth regulation handbook of the Plant Growth Regulation Society of America, 4th edn. Athens, (GA).
- ICI – Imperial Chemical Industries PLC, Plant Protection Division (1986) Clipper. Surrey, UK.
- Rademarcher, W. (2000) Growth retardants: Effects on gibberellins biosynthesis and other metabolic pathways. Annual Review of Plant Physiology and Plant Molecular Biology 51, 501-531.
- Kulkarni, V.J. (1988) Chemical control of tree vigour and promotion of flowering and fruiting in mango (Mangifera indica L.) using paclobutrazol. The Journal of Horticultural Science 63, 557-566.
- Wilkinson, R.I. and Richards, D. (1991) Influence of paclobutrazol on growth and flowering of Rhododrendon ‘Sir Robert Peal’. HortScience 26, 282-284.
- Tukey, L.D. (1983) Vegetative control and fruiting on mature apple trees treated with PP333. Acta Horticulture 137, 103-109.
- Gosh, A., Chikara, J., Chandhari, D.R., Prakashi, A.R., Boricha, G. and Zala, A. (2010) Paclobutrazol arrests vegetative growth and unveils unexpressed yield potential of Jathropa curcas. Journal of Plant Growth Regulation 29: 307-315.
- Early, J.D. and Martin, G.C. (1988) Translocation and breakdown of 14C-labeled paclobutrazol in ‘Nemaguaro’ peach seedlings. HortScience 23: 196-200.
- Muller, C. (2007) Efeito do paclobutrazol na germina??o e no metabolismo do etileno em sementes de Stylosantes humilis HBK. MSc dissertation, Universidade Federal de Vi?osa, Vi?osa.
- Snir, I. (1988) Influence of paclobutrazol on in vitro growth of sweet cherry shoots. HortScience 23, 304-305.
- Panaia, M., Senaratna, T., Bunn, E., Dixon, K.W. and Sivasithamparam, K. (2000) Micropropagation of the critically endangered Western Australian species, Symonanthus bancroftii (F. Muell.) L. Haegi (Solanaceae). Plant Cell Tissue and Organ Culture 63, 23-29.
- Albany, N.R., Vilchez, J.A., Garcia, L. and Jiménez, E. (2005) Comparative study of morphological parameters of Grand Nain banana (Musa AAA) after in vitro multiplication with growth retardants. Plant Cell Tissue and Organ Culture 83, 357-361.
- Haughan, P.A., Burden, R.S., Lenton, J. and Goad, J. (1989) Inhibition of celery cell growth and sterol biosynthesis by the enantiomers of paclobutrazol. Phytochemistry 3, 781-787.
- Chen, J.-T. and Chang W.-C. (2003) Effects of GA3, ancymidol, cycocel and paclobutrazol on direct somatic embryogenesis of Oncidium in vitro. Plant Cell Tissue and Organ Culture 72: 105-108.