Effects of Drought Stress Simulated by Polyethylene Glycol on Seed Germination, Root and Seedling Growth, and Seedling Antioxidant Characteristics in Job’s Tears — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Effects of Drought Stress Simulated by Polyethylene Glycol on Seed Germination, Root and Seedling Growth, and Seedling Antioxidant Characteristics in Job’s Tears
Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
,
Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
,
Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
,
Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
,
Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
,
Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
,
Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
,
Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
1 Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
2 Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
3 Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
4 Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
5 Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
6 Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
7 Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
8 Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang, China
Two Job’s tears cultivars, yy18-1 (high resistance to drought stress) and yy12-7 (susceptible to drought stress) were used to investigate the responses of seed germination, root and seedling growth, and seedling antioxidant characteristics to drought stress simulated by polyethylene glycol (PEG) 6000 solutions with 0, -0.05, -0.1, -0.15, and -0.2 MPa osmotic potentials. The results showed that the germination energy, germination rate, germination index, root and seedling lengths, root and seedling diameters, root and seedling fresh masses, root and seedling dry masses, and seedling relative water content (RWC) decreased with the decrease of the osmotic potential of PEG 6000 solution. The contents of hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), and proline in seedling increased with the decrease of the osmotic potential of PEG 6000 solution. The activities of peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) in seedling increased and then decreased with the decrease of osmotic potential of PEG 6000 solution. -0.1 MPa was the optimal osmotic potential of PEG 6000 solution simulated drought stress at germination stage for Job’s tears. The proline content and activities of POD and CAT were important mechanisms for the maintenance of drought resistance in Job’s tears seedling.
Apirattananusorn, S., Tongta, S., Cui, S.W. and Wang, Q. (2008) Chemical Molecular, and Structural Characterization of Alkali Extractable Nonstarch Polysaccharides from Job’s Tears. Journal of Agricultural and Food Chemistry, 56, 8549-8557. https://doi.org/10.1021/jf801231y
Manosroi, J., Khositsuntiwong, N. and Manosroi, A. (2014) Biological Activities of Functooligosaccharide (FOS)-Containing Coix lacryma-jobi Linn. Extract. Journal of Food Science and Technology, 51, 341-346. https://doi.org/10.1007/s13197-011-0498-6
Bao, Y., Yuan, Y.Z., Xia, L., Jiang, H., Wu, W.R. and Zhang, X.J. (2005) Neural Lipid Isolated from Endosperm of Job’s Tears Inhibits the Growth of Pancreatic Cancer Cells via Apoptosis, G2/M Arrest, and Regulation of Gene Expression. Journal of Gastroenterology and Hepatology, 20, 1046-1053. https://doi.org/10.1111/j.1440-1746.2005.03864.x
Khodarahmpour, Z. (2011) Effect of Drought Stress Induced by Polyethylene Glycol (PEG) on Germination Indices in Corn (Zea mays L.) Hybrids. African Journal of Biotechnology, 10, 18222-18227. https://doi.org/10.5897/AJB11.2639
Shitole, S.M. and Dhumal, K.N. (2012) Effect of Water Stress by Polyethylene Glycol 6000 and Sodium Chloride on Seed Germination and Seedling Growth of Cassia angustifolia. International Journal of Pharmaceutical Sciences and Research, 3, 528-531.
Akcay, U.C., Ercan, O., Kavas, M., Yildiz, L., Yilmaz, C., Oktem, H.A. and Yucel, M. (2010) Drought-Induced Oxidative Damage and Antioxidant Responses in Peanut (Arachis hypogaea L.) Seedlings. Plant Growth Regulation, 61, 21-28. https://doi.org/10.1007/s10725-010-9445-1
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. and Basra, S.M.A. (2009) Plant Drought Stress: Effects, Mechanisms and Management. Agronomy for Sustainable Development, 29, 185-212. https://doi.org/10.1051/agro:2008021
Gong, H., Zhu, X., Chen, K., Wang, S. and Zhang, C. (2005) Silicon Alleviates Oxidative Damage of Wheat Plants in Pots under Drought. Plant Science, 169, 313-321. https://doi.org/10.1016/j.plantsci.2005.02.023
Almaghrabi, O.A. (2012) Impact of Drought Stress on Germination and Seedling Growth Parameters of Some Wheat Cultivars. Life Science Journal, 9, 590-598.
Basu, S., Roychoudhury, A., Saha, P.P. and Sengupta, D.N. (2010) Comparative Analysis of Some Biochemical Responses of Three Indica Rice Varieties during Polyethylene Glycol-Mediated Water Stress Exhibits Distinct Varietal Differences. Acta Physiologiae Plantarum, 32, 551-563. https://doi.org/10.1007/s11738-009-0432-y
Pei, Z.F., Ming, D.F., Liu, D., Wan, G.L., Geng, X.X., Gong, H.J. and Zhou, W.J. (2010) Silicon Improves the Tolerance to Water-Deficit Stress Induced by Polyethylene Glycol in Wheat (Triticum aestivum L.) Seedlings. Journal of Plant Growth Regulation, 29, 106-115. https://doi.org/10.1007/s00344-009-9120-9
Michel, B.E. and Kaufmann, M.R. (1973) The Osmotic Potential of Polyethylene glycol 6000. Plant Physiology, 51, 914-916. https://doi.org/10.1104/pp.51.5.914
Qin, L., Yang, Y.B., Guan, Y.A., Zhang, H.W., Wang, H.L., Liu, B. and Chen, E.Y. (2013) Identification of Drought Tolerance at Germination Period of Foxtail Millet Cultivars Developed from Different Ecological Regions. Journal of Plant Genetic Resources, 14, 146-151.
Smart, R.E. and Bingham, G.E. (1974) Rapid Estimates of Relative Water Content. Plant Physiology, 53, 258-260. https://doi.org/10.1104/pp.53.2.258
Gietler, M., Nykiel, M. and Zagdańska, B.M. (2016) Changes in the Reduction State of Ascorbate and Glutathione, Protein Oxidation and Hydrolysis Leading to the Development of Dehydration Intolerance in Triticum aestivum L. Seedlings. Plant Growth Regulation, 79, 287-297. https://doi.org/10.1007/s10725-015-0133-z
Bates, L.S., Waldren, R.P. and Teare, I.D. (1973) Rapid Determination of Free Proline for Water-Stress Studies. Plant and Soil, 39, 205-207. https://doi.org/10.1007/BF00018060
Wang, C., Hu, D., Liu, X.B., She, H.Z., Ruan, R.W., Yang, H., Yi, Z.L. and Wu, D.Q. (2015) Effects of Uniconazole on the Lignin Metabolism and Lodging Resistance of Culm in Common Buckwheat (Fagopyrum esculentum M.). Field Crops Research, 180, 46-53. https://doi.org/10.1016/j.fcr.2015.05.009
Vardhini, B.V. and Rao, S.S.R. (2003) Amelioration of Osmotic Stress by Brassinosteroids on Seed Germination and Seedling Growth of Three Varieties of Sorghum. Plant Growth Regulation, 41, 25-31. https://doi.org/10.1023/A:1027303518467
Nakano, Y. and Asada, K. (1981) Hydrogen Peroxide Is Scavenged by Ascorbate-Specific Peroxidase in Spinach Chloroplasts. Plant and Cell Physiology, 22, 867-880.
El-Kassaby, Y.A. and Edwards, D.G.W. (2001) Germination Ecology in Mountain Hemlock (Tsuga mertensiana (Bong.) Carr.). Forest Ecology and Management, 144, 183-188. https://doi.org/10.1016/S0378-1127(00)00370-4
Parvez, S.S., Parvez, M.M., Fujii, Y. and Gemma, H. (2003) Allelopathic Competence of Tamarindus indica L. Root Involved in Plant Growth Regulation. Plant Growth Regulation, 41, 139-148. https://doi.org/10.1023/A:1027387126878
Gholami, M., Rahemi, M. and Kholdebarin, B. (2010) Effect of Drought Stress Induced by Polyethylene Glycol on Seed Germination of Four Wild Almond Species. Australian Journal of Basic and Applied Sciences, 4, 785-791.
Almansouri, M., Kinet, J.M. and Lutts, S. (2001) Effect of Salt and Osmotic Stresses on Germination in Durum Wheat (Triticum durum Desf.). Plant and Soil, 231, 243-254. https://doi.org/10.1023/A:1010378409663
Khayatnezhad, M., Gholamin, R., Jamaati-e-Somarin, S. and Zabihi-e-Mahmoodabad, R. (2010) Effects of Peg Stress on Corn Cultivars (Zea mays L.) at Germination Stage. World Applied Sciences Journal, 11, 504-506.
Du, C.Y., Duan, Z.Y., Pan, Y.H., Lei, B.K., Hu, W.L., Fu, B., Chen, A.Q., Chen, S.H., Yang, Y.Q. and Jin, G.M. (2015) Effect of Drought Stress on Growth and Activities of Antioxidant Enzymes of Maize Seedling. Agricultural Research in the Arid Areas, 33, 124-129.
Ashraf, M. and Foolad, M.R. (2007) Roles of Glycinebetaine and Proline in Improving Plant Abiotic Stress Resistance. Environmental and Experimental Botany, 59, 206-216. https://doi.org/10.1016/j.envexpbot.2005.12.006
Alexieva, V., Sergiev, I., Mapelli, S. and Karanov, E. (2001) The Effect of Drought and Ultraviolet Radiation on Growth and Stress Markers in Pea and Wheat. Plant Cell and Environment, 24, 1337-1344. https://doi.org/10.1046/j.1365-3040.2001.00778.x
Madhusudhan, R., Ishikawa, T., Sawa, Y., Shiqeoka, S. and Shibata, H. (2003) Characterization of an Ascorbate Peroxidase in Plastids of Tobacco BY-2 Cells. Physiologia Plantarum, 117, 550-557. https://doi.org/10.1034/j.1399-3054.2003.00066.x
Pastori, G., Foyer, C.H. and Mullineaux, P. (2000) Low Temperature-Induced Changes in the Distribution of H2O2 and Antioxidants between the Bundle Sheath and Mesophyll Cells of Maize Leaves. Journal of Experimental Botany, 51, 107-113. https://doi.org/10.1093/jexbot/51.342.107