Pineapple is nutrient and planted in many tropical and subtropical countries. Plenty of elite seedlings were always needed in short time. The common methods for propagating pineapple seedlings have low-multiplying index or take long time. A new method was introduced in this paper. Shoots were induced using stem sections. And then, the shoot bases were cut and cultured on callus-induced medium. The meristematic granules induced were cultured on shoot-induced medium. Shoots can be induced from the meristematic granules. Seedlings can be induced from these shoots in short time. This method overcomes the defects of the common ways. Enough seedlings can be gotten in short time. The operation is simple and easy to learn.
Akbar, M.A., Karmakar, B.K. and Roy, S.K. (2003) Callus Induction and High-Frequency Plant Regeneration of Pineapple (Ananas comosus (L.) Merr.). Plant Tissue Culture, 13, 109-116.
He, J.H., Chen, H.R., Huang, H.J., Wang, J.H. and Huang, H.N. (2015) The New Varieties and High-Quality- and High-Production-Cultivation Technology of Pineapple (Ananas comosus L.). China Agricultural Science and Technology Press, Beijing.
Shu, H., Sun, W., Li, K., Xu, G., Zhan, R. and Chang, S. (2019) The Cause for Water-Heart Fruit of Pineapple and Protective Measurements. American Journal of Plant Sciences, 10, 885-892. https://doi.org/10.4236/ajps.2019.106063
Shu, H., Sun, W., Li, K., Xu, G., Zhan, R. and Chang, S. (2019) The Situation and Challenges of Pineapple Industry in China. Agricultural Sciences, 10, 683-688. https://doi.org/10.4236/as.2019.105053
Benega, R., Isidron, M.J., Hidalgo, M. and Borroto, C.G. (1995) In Vitro Regeneration and Callus Formation in Pineapple (Ananas comosus (L.) Merr.) Hybrid Seed. Acta Horticulturae, 425, 215-220. https://doi.org/10.17660/ActaHortic.1997.425.26
Cote, F.R., Domergue, R., Follit, M., Bouffin, J. and Marie, F. (1991) In Vitro Micropropagation of Pineapple. Fruits Paris, Numero Special Ananas, 46, 359-366.
Daquinta, M., Castillo, R., Lorenzo, C., Lunas, I., Escalona, M., et al. (1994) Formation de callos en pina (Ananas comosus (L.) Merr.). Revista Brasileira de Fruticultura, 16, 83-91.
De Wald, M.G. (1988) Tissue Culture and Electrophoretic Studies of Pineapple (Aananas comosus) and Related Species. International B, Science and Engineering, 48, 8.
Khatun, M.M., Khanam, D., Hoque, M.A. and Quasem, A. (1997) Clonal Propagation of Pineapple through Tissue Culture. Plant Tissue Culture, 7, 143-148.
Lakshmi, S.G., Singh, R. and Layer, C.P.A. (1974) Plantlets through Shoot Tip Culture in Pineapple. Current Science, 43, 724.
Mathews, V.H. and Rangan, T.S. (1979) Multiple Plantlets in Lateral Buds and Leaf Explants in in Vitro Cultures of Pineapple. Scientia Horticulturae, 11, 319-326. https://doi.org/10.1016/0304-4238(79)90016-5
Rahman, K.W., Amin, M.N. and Azad, M.A.K. (2001) In Vitro Rapid Propagation of Pineapple, Ananas comosus (L.) Merr. Plant Tissue Culture, 11, 47-53.
Roostika, I., Khumaida, N., Mariska, I. and Wattimena, G.A. (2012) The Effect of Picloram and Light on Somatic Embryogenesis Regeneration of Pineapple. Indonesian Journal of Agricultural Science, 13, 43-53.
Aydieh, A.A., Ibrahim, M.K.H. and Ibrahim, A. (2000) In Vitro Propagation and Fruiting of Pineapple. Egyptian Journal of Horticulture, 27, 289-304.
Be, L.V. and Debergh, P.C. (2006) Potential Low Cost Micropropagation of Pineapple (Ananas comosus). South African Journal of Botany, 72, 191-194. https://doi.org/10.1016/j.sajb.2005.07.002
Bhatia, P. and Ashwath, N. (2002) Development of Rapid Method for Micropropagation of a New Pineapple (Ananas comosus (L.) Merr. Clone Yeppoon Gold. Acta Horticulturae, 575, 125-131. https://doi.org/10.17660/ActaHortic.2002.575.11
Firoozabady, E. and Gutterson, N. (2003) Cost Effective in Vitro Prapagation Methods for Pineapple. Plant Cell Reports, 21, 844-850.
Sripaoraya, S., Marchant, R., Power, J.B. and Davey, M.R. (2003) Plant Regeneration by Somatic Embryogenesis in Commercial Pineapple (Ananas comosus L.). In Vitro Cellular & Developmental Biology-Plant, 39, 450-454. https://doi.org/10.1079/IVP2003445
Zepeda, C. and Sagawa, Y. (1981) In Vitro Propagation of Pineapple. Horticultural Science, 16, 495.
Al-Saif, A.M., Hossain, A.B.M.S. and Taha, R.M. (2011) Effects of Benzylaminopurine and Naphthalene Acetic Acid on Proliferation and Shoot Growth of Pineapple (Ananas comosus L. Merr) in Vitro. African Journal of Biotechnology, 10, 5291-5295. https://doi.org/10.5897/AJB11.370
Jiang, L. (2006) Study on Establishment of Genetic Transformation System of Pineapple (Ananas comosus (L) Merr) Tainong-16 and Gene Transfer of Resveratrol Synthase into Pineapple. Chinese Agricultural University, Beijing.
Xu, Y. (2006) Studies on Rapid Propagation and Polyploid Breeding of Ananas comosus (L.) Merrill. Southwest University, Chongqing, China.
Firoozabady, E. and Moy, Y. (2004) Regeneration of Pineapple Plants via Somatic Embryogenesis and Organogenesis. In Vitro Cellular & Developmental Biology-Plant, 40, 67-74. https://doi.org/10.1079/IVP2003494
Iwase, A., Ohme-Takagi, M. and Sugimoto, K. (2011) WIND1, A Key Molecular Switch for Plant Cell Dedifferentiation. Plant Signaling & Behavior, 6, 1943-1945. https://doi.org/10.4161/psb.6.12.18266
Strosse, H., Domergue, R., Panis, B., Escalant, J. and Cote, F. (2001) Banana and Plantain Embryogenic Cell Suspensions. INIBAP Technical Guidelines, Montpellier Cedex.
Cote, F.X., Domergue, R., Monmarson, S., Schwendiman, J., Teisson, C., et al. (1996) Embryogenic Cell Suspensions from the Male Flower of Musa AAA cv. Grand Nain. Physiologia Plantarum, 97, 285-290. https://doi.org/10.1034/j.1399-3054.1996.970211.x