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The Use of DNA Extraction for Molecular Biology and Biotechnology Training: A Practical and Alternative Approach
Post-Graduation Program of Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Brazil
Biophysics and Biometry Departament, Roberto Alcantara Gomes Biolology Institute, Rio de Janeiro State University, Rio de Janeiro, Brazil
Post-Graduation Program of Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Brazil
Post-Graduation Program of Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Brazil
Post-Graduation Program of Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Brazil
- 1 Post-Graduation Program of Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Brazil
- 2 Biophysics and Biometry Departament, Roberto Alcantara Gomes Biolology Institute, Rio de Janeiro State University, Rio de Janeiro, Brazil
- 3 Post-Graduation Program of Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Brazil
- 4 Post-Graduation Program of Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Brazil
- 5 Post-Graduation Program of Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Brazil
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Abstract
DNA extraction and polymerase chain reaction are molecular biology techniques widely used in research, diagnostics and biotechnology industry. In this work, we report a practical course designed for undergraduate students of health, biological sciences and biotechnology areas. The course is five days long and emphasizes basic procedures in practical activities. The students were challenged to use three different protocols for DNA isolation (one using commercial silica column and two, in-house) using no lab animals but themselves. They evaluated the DNA yield, and potential amplification of the extracted DNA by PCR using their own cells genetic material. Students were stimulated to adopt a critical view during the course to develop skills including critical analysis of primary results and possible errors, scientific report writing and a problem solving profile.
KeywordsMolecular BiologyPractical CourseDNA ExtractionWhole BloodPolymerase Chain Reaction
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