The present study investigated the effect of an herbal extract-supplemented cardiomyopeptin preparation (HECP), in the rat model of chronic heart failure. HECP pre-/co-treatment at a daily dose of 0.072 to 0.124 g/kg for 30 days protected against isoproterenol (ISO)-induced chronic myocardial damage in rats in a dose-dependent manner, with the extent of protection, as assessed by plasma cardiac troponin I level, being up to 76%. The cardioprotection afforded by HECP was associated with the enhancement of myocardial mitochondrial antioxidant status, amelioration of plasma parameters on cardiac dysfunction and hypertrophy, as well as an increase in myocardial endothelial nitric oxide synthase activity. Myocardial apoptotic and anti-apoptotic parameters were suppressed and stimulated, respectively. The cardioprotection afforded by HECP was accompanied by an increase in exercise capacity, an indirect functional index of the myocardium, in ISO-challenged rats. In conclusion, HECP may offer a promising prospect in preventing chronic heart failure in human subjects.
Ferdinandy, P., Schulz, R. and Baxter, G.F. (2007) Interaction of Cardiovascular Risk Factors with Myocardial Ischemia/Reperfusion Injury, Preconditioning, and Postconditioning. Pharmacological Reviews, 59, 418-458. https://doi.org/10.1124/pr.107.06002
Zhou, R., Ma, P., Xiong, A., Xu, Y., Wang, Y. and Xu, Q. (2016) Protective Effects of Low-Dose Rosuvastatin on Isoproterenol-Induced Chronic Heart Failure in Rats by Regulation DDAH-ADMA-NO Pathway. Cardiovascular Therapeutics, 35, e12241. https://doi.org/10.1111/1755-5922.12241
San, H., Liu, L., Zhang, L., Meng, Q. and Du, J. (2018) Creative Optimization and Industrial Research of Freeze Drying Process of the Cardiomyopeptidin for Injection. Chemical Engineering Transactions, 70, 1201-1206.
Kong, X., Wan, H., Li, R., Yi, X. and Xu, Z. (2000) Effects of Cardiomyopeptidin on Anoxia-Reoxygenation Injury in Cultured Neonatal Rat Myocardial Cells. Chinese Pharmacological Bulletin, 16, 52-54.
Wan, H., Kong, X. Yang, L. Li, R. and Zhang, Y. (2001) Protective Effect of Cardiomyopeptidin on Cultured Myocardial Cells Injured by Anoxia-Reoxygenation. Chinese Journal of Pathophysiology, 17, 481-484.
Yang, L.P., Wan, H.Y., Kong, X.P., Wu, Y., Teng, J. and Fan, L.L. (2000) Preventive Effect of Cardiomyopeptidin on Rat Heart Injured by Ischemia-Reperfusion. China Journal of Chinese Materia Medica, 25, 105-107.
Hao, P., Jiang, F., Cheng, J., Ma, L., Zhang, Y. and Zhao, Y. (2017) Traditional Chinese Medicine for Cardiovascular Diseases: Evidence and Potential Mechanisms. Journals of the American College of Cardiology, 69, 2952-2966. https://doi.org/10.1016/j.jacc.2017.04.041
Li, J., Ding, Y., Wang, C. and Ma, Y. (2016). New Development of Traditional Chinese Medicine on Myocardial Ischemia-Reperfusion Injury. Acta Chinese Medicine Pharmacology, 44, 105-107.
Krenek, P., Kmecova, J., Kucerova, D., Bajuszova, Z., Musil, P., Gazova, A., Ochodnicky, P., Klimas, J. and Kyselovic, J. (2009) Isoproterenol-Induced Heart Failure in the Rat Is Associated with Nitric Oxide-Dependent Functional Alterations of Cardiac Function. European Journal of Heart Failure, 11, 140-146. https://doi.org/10.1093/eurjhf/hfn026
Ko, K.M., Chiu, P.Y., Leung, H.Y., Siu, A.H., Chen, N., Leong, E.P. and Poon, M.K. (2010) Long-Term Dietary Supplementation with a Yang-Invigorating Chinese Herbal Formula Increases Lifespan and Mitigates Age-Associated Declines in Mitochondrial Antioxidant Status and Functional Ability of Various Tissues in Male and Female C57BL/6J Mice. Rejuvenation Research, 13, 168-171. https://doi.org/10.1089/rej.2009.0893
Sun, M., Yu, H., Zhang, Y., Li, Z. and Gao, W. (2015) MicroRNA-214 Mediates Isoproterenol-Induced Proliferation and Collagen Synthesis in Cardiac Fibroblasts. Scientific Reports, 5, Article No. 18351. https://doi.org/10.1038/srep18351
Chen, H., Xu, Y., Wang, J., Zhao, W. and Ruan, H. (2015) Baicalin Ameliorates Isoproterenol-Induced Acute Myocardial Infarction through iNOS, Inflammation and Oxidative Stress in Rat. International Journal of Clinical and Experimental Pathology, 8, 10139-10147.
Kim, K., Chini, N., Fairchild, D.G., Engle, S.K., Reagan, W.J., Summers, S.D. and Mirsalis, J.C. (2016) Cardiac Hypertrophy Working Group of the Predictive Safety Testing Consortium: Evaluation of Cardiac Toxicity Biomarkers in Rats from Different Laboratories. Toxicologic Pathology, 44, 1072-1083. https://doi.org/10.1177/0192623316668276
Wong, S.M., Chiu, P.Y., Leung, H.Y., Zhou, L., Zuo, Z., Lam, P.Y. and Ko, K.M. (2011) Myocardial Post-Conditioning with Danshen-Gegen Decoction Protects against Isoproterenol-Induced Myocardial Injury via a PKCε/mKATP-Mediated Pathway in Rats. Chinese Medicine, 6, Article No. 7. https://doi.org/10.1186/1749-8546-6-7
Novaes, R.D., Gonçalves, R.V., Penitente, A.R., Bozi, L.H., Neves, C.A., Maldonado, I.R., Natali, A.J. and Talvani, A. (2016) Modulation of Inflammatory and Oxidative Status by Exercise Attenuates Cardiac Morphofunctional Remodeling in Experimental Chagas Cardiomyopathy. Life Science, 152, 210-219. https://doi.org/10.1016/j.lfs.2016.03.053
Lecour, S. (2009) Activation of the Protective Survivor Activating Factor Enhancement (SAFE) Pathway against Reperfusion Injury: Does It Go beyond the Risk Pathway? Journal of Molecular and Cellular Cardiology, 47, 32-40. https://doi.org/10.1016/j.yjmcc.2009.03.019
Zhou, R., Xu, Q., Zheng, P., Yan, L., Zheng, J. and Dai, G. (2008) Cardioprotective Effect of Fluvastatin on Isoproterenol-Induced Myocardial Infarction in Rat. European Journal of Pharmacology, 586, 244-250. https://doi.org/10.1016/j.ejphar.2008.02.057
Nivethetha, M., Jayasri, J. and Brindha, P. (2009) Effects of Muntingia calabura L. on Isoproterenol-Induced Myocardial Infarction. Singapore Medical Journal, 50, 300-302.
Senthil, S., Sridev,i M. and Pugalendi, K.V. (2007) Cardioprotective Effect of Oleanolic Acid on Isoproterenol-Induced Myocardial Ischemia in Rats. Toxicologic Pathology, 35, 418-423. https://doi.org/10.1080/01926230701230312
Lobo, R.O. and Shenoy, C.K. (2015) Myocardial Potency of Bio-Tea against Isoproterenol Induced Myocardial Damage in Rats. Journal of Food Science and Technology, 52, 4491-4498. https://doi.org/10.1007/s13197-014-1492-6
Chiu, P.Y., Wong, S.M., Leung, H.Y., Leong, P.K., Chen, N., Zhou, L., Zuo, Z., Lam, P.Y. and Ko, K.M. (2011) Acute Treatment with Danshen-Gegen Decoction Protects the Myocardium against Ischemia/Reperfusion Injury via the Redox-Sensitive PKCε/mKATP Pathway in Rats. Phytomedicine, 18, 916-925. https://doi.org/10.1016/j.phymed.2011.03.006
Chen, L., Shi, D. and Guo, M. (2021) The Roles of PKC-δ and PKC-ε in Myocardial Ischemia/Reperfusion Injury. Pharmacological Research, 170, Article ID: 105716. https://doi.org/10.1016/j.phrs.2021.105716
Heusch, G. (2015) Molecular Basis of Cardioprotection: Signal Transduction in Ischemic Pre-, Post-, and Remote Conditioning. Circulation Research, 116, 674-699. https://doi.org/10.1161/CIRCRESAHA.116.305348
Lee, C.H. and Kim, J.H. (2014) A Review on the Medicinal Potentials of Ginseng and Ginsenosides on Cardiovascular Diseases. Journal of Ginseng Research, 38, 161-166. https://doi.org/10.1016/j.jgr.2014.03.001
Zhu, X., Wu, W., Chen, X., Yang, F., Zhang, J. and Hou, J. (2018) Protective Effects of Polygonatum sibiricum Polysaccharide on Acute Heart Failure in Rats. Acta Cirúrgica Brasileira, 33, 868-878. https://doi.org/10.1590/s0102-865020180100000001
Rosales, S.L.O., Anog, B.F.A., Blanza, R.M., Cuartero, M.A.D., Landicho, M.C., Talledo, E.G.D., Cabanela, R.A., Dumaoal, O.S. R. and Magbojos, C.R. (2013) Cardioprotective Activity of Bambusa blumeana Schultes Leaf Crude Extract against Isoproterenol Induced Myocardial Infarction in Sprague-Dawley Rats. The Steth, 7, 41-57.
Lu, N., Sun, Y. and Zheng, X. (2011) Orientin-Induced Cardioprotection against Reperfusion Is Associated with Attenuation of Mitochondrial Permeability Transition. Planta Medica, 77, 984-991. https://doi.org/10.1055/s-0030-1250718