Effect of Sijunzi Decoction on the Myonuclear Domain of Rat Soleus in Spleen Qi Deficiency
- 1 Department of Physiology and Psychology, College of Integrated Chinese and Western medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 2 Department of Physiology and Psychology, College of Integrated Chinese and Western medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 3 Department of Physiology and Psychology, College of Integrated Chinese and Western medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 4 Department of Physiology and Psychology, College of Integrated Chinese and Western medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 5 Department of Physiology and Psychology, College of Integrated Chinese and Western medicine, Liaoning University of traditional Chinese Medicine, Shenyang, China
- 6 Department of Physiology and Psychology, College of Integrated Chinese and Western medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, China
Abstract
Objective : To study the mechanism of Sijunzi decoction treating limb weakness in spleen Qi deficiency (SQD) based on the myonuclear domain (MND) theory. Methods : 40 male Sprague-Dawley rats were randomly divided into the normal group, SQD model group (model group), SQD+ still water group (SW group) and SQD+ Sijunzi decoction group (CM group), 10 rats each group; Grip-Strength Meter was used to measure limb grip strength; transmission electron microscope was employed to observe the ultrastructural changes of the myofibers, Image Pro 6.0 was used to measure the myonuclear numbers, cross-section area (CSA) and then their ratios (the MND sizes) were calculated, immunofluorescence assay was chosen to test the expressions of paired box gene 7 (Pax7) and myogenic differentiation antigen (MyoD). Results : Compared with those in the normal group, limb grip strength was decreased, sarcomeres were abnormal, and all the myonuclear numbers, CSA and MND sizes were reduced, but the Pax7+ cell numbers were increased, significantly, in the model and SW groups; Compared with those in the model and SW groups, limb grip strength was increased, sarcomeres were basically normal, the myonuclear number and CSA were both greater, and the Pax7+ and MyoD+ cell numbers were both increased, significantly, in the CM group. Conclusion : Sijunzi decoction might increase the myonuclear number by activating the MSCs to treat limb weakness in SQD.
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