Effects of the Supercritical Fluid Extraction of Dahurian Angelica Root and Szechwan Lovage Rhizome on Spontaneous Hypertension Rats
- 1 The Mental Health Center of Xi’an, Xi’an, China
- 2 The Mental Health Center of Xi’an, Xi’an, China
- 3 School of Medicine, Xi’an Jiaotong University, Xi’an, China
- 4 School of Medicine, Xi’an Jiaotong University, Xi’an, China
- 5 No. 5 Hospital of Xi’an, Xi’an, China
Abstract
The supercritical fluid extraction of Dahurian Angelica Root (Bai Zhi) and Szechwan Lovage Rhizome (Chuan Xiong) was named as BCC. In the study, we investigated whether BCC had effects on left ventricular hypertrophy (LVH) and myocardial fibrosis in spontaneous hypertensive rats (SHR). For SHR + BCC group, BCC (0.3 g/kg) was orally admin istered daily for 12 weeks. The SHR group and the Wistar Kyoto rats (WKY, normal control) group, the equal volume of 5‰ CMC-Na distilled water. After 12 weeks, left ventricle was segregated from each rat in the groups, and the left ventricle weight/body weight (LVW/BW) calculated. The volume fraction of collagen (VFC) in myocardium and the diameter of cardiac muscle cell (DCMC) were examined by histological staining. Biochemical indicators of blood sam ple such as Angiotensin II (Ang II), Aldosterone (ALD), Hyaluronic Acid (HA), Laminin (LN), Procollagen III (PC III) and Collagen type IV (CIV) levels were detected by using radioimmunoassay (RIA). And also NOS and iNOS levels were measured by means of ultra-violet spectroscopy (UV). The results shown that in SHR + BCC group, the LVW/BW, DCMC and VFC decreased significantly versus SHR group, the same as biochemical indicators except NOS and iNOS. All of above index was similar to WKY group. Statistically significant correlations were found among the plasma Ang II level, the mean systolic blood pressure (SBP), and the NOS level of the three groups. Our study indicates that the BCC can control the LVH and myocardial fibrosis in SHR.
- K. Michaela, B. Simona, P. Carlo, B. Giampaolo, M. Angelica, F. Stefania, et al., “Myocardial Ultrasonic Backscatter in Hypertension Relation to Aldosterone and Endothelin,” Hypertension, Vol. 41, No. 2, 2003, pp. 230-236. doi:10.1161/01.HYP.0000052542.68896.2B
- Y. Hu and N. S. Cai, “Reconstitution of LVH in High Blood Pressure,” Chinese Journal of Internal Medicine, Vol. 36, No. 6, 1997, pp. 424-426.
- L. M. De, A. Estevez, D. Bunout, C. Klenner, M. Oyonarte and S. Hirsch, “Ventricular Mass in Hypertensive and Normotensive Obese Subjects,” International Journal of Obesity and Related Metabolic Disorders, Vol. 18, No. 4, 1994, pp. 193-197.
- M. S. Lauer, K. M. Anderson and D. Levy, “Separate and Joint Influences of Obesity and Mild Hypertension on Left Ventricular Mass and Geometry: The Framingham Heart Study,” Journal of the American College of Cardiology, Vol. 19, No. 1, 1992, pp. 130-134. doi:10.1016/0735-1097(92)90063-S
- C. Li and F. Lu, “Change of Myocardial Interstitial Fibrosis Index in Hypertension Complicated with Left Ventricular Hypertrophy and Influence of ACET Therapy,” Chinese Journal of Cardiovascular Rehabilitation Medicine, Vol. 12, No. 1, 2003, pp. 22-24.
- C. G. Brilla, G. Zhou, L. Matsubara and K. T. Weber, “Collagen Metabolism in Cultured Adult Rat Cardiac Fibroblasts: Response to Angiotensin II and Aldosterone,” Journal of Molecular and Cellular Cardiology, Vol. 26, No. 7, 1994, pp. 809-820. doi:10.1006/jmcc.1994.1098
- F. J. Villarreal, N. N. Kim, G. D. Ungab, M. P. Printz and W. H. Dillmann, “Identification of Functional Angiotensin II Receptors on Rat Cardiac Fibroblasts,” Circulation, Vol. 88, No. 6, 1993, pp. 2849-2861. doi:10.1161/01.CIR.88.6.2849
- W. Sibylle, R. Cornelia, W. Sandra, R. Joachim, K. Georg and S. Klaus-Dieter, “Lack of Endothelial Nitric Oxide Synthase-Derived Nitric Oxide Formation Favors Hypertrophy in Adult Ventricular Cardiomyocytes,” Hypertension, Vol. 49, No. 1, 2007, pp. 193-200.
- H. C. Shih, T. H. Lee, S. C. Chen, C. Y. Li and T. Shibuya, “Antihypertension Effects of Traditional Chinese Medicine Ju-Ling-Tang on Renal Hypertensive Rats,” The American Journal of Chinese Medicine, Vol. 33, No. 6, 2005, pp. 913-921. doi:10.1142/S0192415X05003545
- W. H. Zhao, Y. X. Cao, J. Liu and L. C. He, “The Influence of Bai Chuan Capsule to Awake Rats,” Journal of Xi’an Medical University, Vol. 22, No. 4, 2001, pp. 315-336.
- Y. Zhang, L. C. He, H. J. Duan and Y. Z. Zhan, “Effects of Bai Chuan Capsule on Left Ventricular Hypertrophy and Correlative Indexes,” Journal of Chinese Medicinal Materials, Vol. 33, No. 8, 2010, pp. 1290-1292.