Metabolic and Behavioral Patterns in a Pre-Menstrual Syndrome Animal Model with Liver-qi Invasion and Their Reversal by a Chinese Traditional Formula
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Abstract
This work successfully used model rats with Pre-Menstrual Syndrome (PMS) liver-qi invasion in the early de-velopment days to detect the Metabolic and Behavioral Patterns and their reversal by a Chinese traditional for-mula. Our aim is to verify the reliability of PMS liver-qi invasion rat model and explore some micro- mecha-nism of the syndrome of the liver failing to maintain the normal flow of qi. 30 rats with estrous cycles not in accepting time were selected and divided randomly into three groups: the normal control group, PMS liver-qi invasion model group and PMS liver-qi invasion medication-administered group. Emotional stimulation and multiple factors combination were used to prepare the PMS liver-qi invasion model. Baixiangdan Capsules (a Chinese traditional formula) were administered to rats to interfere with the PMS liver-qi invasion mode. Open-field test was used to explore behavioral aspects of the model. Urine samples, from the three groups, were collected and analyzed with UPLC-Q-TOF method to detect changes in metabolites related to liver func-tions. In the open-field experiment, the crossing scores, rearing scores and open-field experiment total scores of rats in the PMS liver-qi invasion model group increased remarkably (P < 0.05) compared with the scores of the normal control group, the tendency was retrieved remarkably after medications (P < 0.05). Metabolic finger-prints between the PMS liver-qi invasion model group and the normal control group had also distinguished changes through principal component analysis, and an evident restoration trend occurred after Baixiangdan Capsules administration. Taken together, behavioral and metabolic patterns can differentiate the PMS liver-qi invasion rat models from the normal rats. Our results identified potential biological markers that might reflect metabolic pathologies associated with PMS liver-qi invasion.
- J. J. Li, “Clinical Gynecologic Endocrinology and Dysgenesia,” Shandong Science and Technology Publishing Company, Jinan, 2003.
- T. Schneider and P. Popik, “Increased Depress Ive-Like Traits in All Animal Model of Premenstrual Irritability,” Horm behav, Vol. 51, No. 1, January 2007, pp. 142-148.
- S. Wei, “Improvement of Premenstrual Syndrome Liver- Qi Invasion, Dpression Animal Model and Study on Micro-mechanism of the Liver Failing to Maintain the Normal Flow of Qi,” Journal of Shandong University of Traditional Chinese Medicine, Vol. 31, No. 5, May 2007, pp. 1-5.
- X. F. Tu, “Effects of Excessive Swimming Exercise Oil the Estrous Cycle of Mice,” Chinese Journal of Clinical Rehabilitation, Vol. 36, No. 8, Augest 2004, pp. 8316- 8317.
- H.-P. Ho, Marie Olsson and Lars Westberg, “The Serotonin Reuptake Inhibitor Fluoxetine Reduces Sex Steroid-Related Aggression in Female Rats: An Animal Model of Premenstrual Irritability?” Neuropsychopharmacology, Vol. 24, No. 5, May 2001, pp. 502-510.
- H. Y. Zhang, M. Q. Qiao and W. C. Zhu, “Variation on Rats' Serum Hormones and Behavior of Premenstrual Syndrome Liver-Qi Invasion,” Chinese Traditional Patent Medicine, Vol. 24, No. 2, February 2002, pp. 118-119.
- S. Y. Xu, R. L. Bian and X. Chen, “Pharmacological Experiments Methodology,” People's Medical Publishing House, Beijing, 2002.
- X. W. Liu, H. M. Zhang and H. D. Qu, “Observation and Detection of Behavior on Rat of ‘Anger Hurt Qi’,” Jiang Su Journal of Traditional Chinese Medicine, Vol. 26, No. 3, March 2005, pp. 53-55.
- L. Sun, “PMS Rat Model of Liver against the Permit and Different Brain Regions of Serum Progesterone and Amino Acid Detection Analysis,” Master dissertation, Shandong University of Traditional Chinese Medicine, Jinan, 2008.
- C. Konradi and S. Heckers, “Molecular Aspects of Glutamate Dysregulation: Implications for Schizophrenia and Its Treatment,” Pharmacology & Therapeutics, Vol. 97, No. 2, February 2003, pp. 153-179.
- X. Zhou and X. Q. Wang, “Glutamate and Γ-Aminobutyric Acid System in Mood Disorder,” Chinese Journal of Neuroscience, Vol. 19, No. 2, February 2003, pp. 130-133.