Expression Imbalance of Cholinergic M<sub>2</sub> and M<sub>3</sub> Receptors Contributes to the Motility Reduction of the Small Intestine in Spleen Qi Deficiency
- 1 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 2 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 3 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 4 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 5 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 6 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 7 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 8 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 9 Liaoning University of Traditional Chinese Medicine, Shenyang, China
- 10 Liaoning University of Traditional Chinese Medicine, Shenyang, China
Abstract
Objective: To study roles of cholinergic M 2 and M 3 receptors in the motility reduction of small intestine (SI) in spleen qi deficiency. Methods: 16 male SD rats were randomly divided in the control group and spleen qi deficiency group (model group)—8 rats each group; spleen qi deficiency model of the improper diet and overfatigue was established; the SI propelling rate (SIPR) was used to evaluate the SI motility; ELISA was used to measure concentrations of acetylcholine (ACh), cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) in the SI tissue; immohistochemistry was employed to detect expressions of cholinergic M 2 and M 3 receptors. Results: Compared with those in the control group, SIPR was reduced; expression of M 2 receptors was increased; and expression of M 3 receptors and concentrations of cAMP and PKA were decreased, significantly, in the model group. Conclusions: Expression imbalance of cholinergic M 2 and M 3 receptors might contribute to the motility reduction of the SI in spleen qi deficiency.
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