The Mechanism of <i>Cornus officinalis</i> Total Glycosides and Cornus Polysaccharide on Myocardial Protection in Rats with Acute Myocardial Infarction

Abstract

Objective: To investigate effects of Cornus officinalis Total Glycosides (COTG) and Cornus Polysaccharide (CP) on myocardial protection and on expression of mitochondria biogenesis related gene of acute myocardial infarction (AMI) rats, Materials and Methods: Ninety-six SD rats of SPF level were randomly divided into 5 groups: sham operation group, model group, preventive treatment group, COTG treatment group, CP treatment group, and there were 12 cases in each one. By legating the left anterior descending branch of coronary artery method, acute myocardial infarction model was established. The rat of sham operation group and model group was intragastric administered with physiological saline; other groups were given with corresponding drugs. The cardiac function, the myocardial infarct area, the expression of mitochondrial biogenesis genes such as PGC-1 α , PGC-1 β , NRF-1mRNA and GSK-3 β mRNA, GSK-3 β Protein Expression were analyzed. Results: The results revealed that compared with model group, myocardial infarction size, LVDs, LVDd, LVESV, LVEDP, and - dp/dt decreased; LVSP increased in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05); LVEDV increased in preventive treatment group (p < 0.05), PGC 1 alpha, and PGC 1 beta; the NRF-1 mRNA expression increased in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared with CP and COTG treatment group, PGClpha, beta PGC 1, the NRF-1 mRNA expression increased in preventive treatment group (p < 0.05). Compared with the sham operation group, GSK-3 beta mRNA and protein expression increased in model group, preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared with model group, GSK-3 beta mRNA expression reduced in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Conclusions: Cornus officinalis total glycosides and Cornus polysaccharides can effectively protect myocardial mitochondria of acute myocardial infarction rats by activating GSK-3 β signaling pathways, and reduce the myocardial infarct size, which has great significance for improving cardiac function.