Effects of Nourishing “Yin”-Removing “Fire” Chinese Herb Mixture on the Expression of GABAB Receptors in Hypothalamus of Precocious Puberty Female Rats — Oak Academic Publishing
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Effects of Nourishing “Yin”-Removing “Fire” Chinese Herb Mixture on the Expression of GABAB Receptors in Hypothalamus of Precocious Puberty Female Rats
Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
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Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
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Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
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Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
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Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
1 Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
2 Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
3 Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
4 Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
5 Department of Integrative Medicine and Neurobiology, Institute of Acupuncture Research, Institutes of Brain Science, Collaborative Innovation Center for Brain Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai, China
Objectives: To investigate the potential role of GABAB Receptors (GABABRs) involved in the effect of Nourishing “Yin”-Removing “Fire” Chinese herb mixture (TCM) treatment on precocious puberty. Methods: Female Sprague-Dawley rats were randomly divided into four groups: normal (N), central precocious puberty (CPP) model (M), CPP fed with normal saline (S) and CPP fed with Nourishing “Yin”-Removing “Fire” Chinese herb mixture (TCM). Rats of postnatal day 5 were given a single subcutaneous injection of 240 μg danazol to establish CPP model rats. Rats of S and TCM groups were continuously administered with saline or nourishing “Yin”-removing “Fire” Chinese herb mixture since postnatal day 15. The expression of GABABRs was detected by means of real-time PCR and immunohistochemistry. Results: The expression of hypothalamic GnRH mRNA in M was significantly increased on the day of pre-puberty when compared with that of N (P < 0.01). On the day of onset-puberty, LH levels were higher in M than those in N (P < 0.01), while the serum E2 and LH levels of TCM decreased when compared with those of M (P < 0.05). On the day of pre-puberty, the number of GABA B1 receptor (GABABR1) immunoreactive cells in the arcuate nucleus (ARN) was decreased in M when compared with that of N (P < 0.05) and increased in TCM compared with that of M (P < 0.05); simultaneously, the expression of GABABR1 mRNA in hypothalamus was significantly decreased in M when compared with that of N (P < 0.01) and increased in TCM compared with that of M (P < 0.01). On the day of onset-puberty, the number of GABABR1 immunoreactive cells in medial septum (MS) was decreased in M compared with that in N (P < 0.05) and increased in TCM comparing with that of M (P < 0.05); meanwhile, the mRNA expression of GABABR1 in hypothalamus was decreased in M compared with that in N (P < 0.05), while the numbers of GABA B2 receptor (GABABR2) immunoreactive cells in the ARN, MS and diagnonal band of broca (DBB) were decreased in M when compared with those of N (P < 0.05) and increased in TCM compared with those of M (P < 0.05) on the day of onset-puberty. At the same time, the mRNA expression of GABABR2 was significantly decreased in M compared with that of N (P < 0.01). Conclusions: These results indicated that the GABABRs might involve in the curative effect of Nourishing “Yin”-Removing “Fire” Chinese herbal mixture on CPP rats.
KeywordsPrecocious PubertyGABAB ReceptorsChinese Herb MedicineDanazol
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