Engineering of Carbonate Apatite Bone Substitute Based on Composition-Transformation of Gypsum and Calcium Hydroxide
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Abstract
Even though a lot of research has been carried out concerning the preparation of carbonate apatite (CHA), they were related to CHA in the form of powder. In the present study, macroporous CHA bone substitutes were prepared through composition-transformation of gypsum and Ca-hydroxide. Here, we investigated the effect of added Ca-hydroxide to gypsum, carbonation periods, and hydrothermal temperatures for phosphatization to understand the basic principle of composition-transformation of gypsum added Ca-hydroxide to fabricate CHA bone substitutes. The specimens were characterized in terms of chemical and physical properties, such as extent of transformation of macroporous gypsum added Ca-hydroxide into CHA body, type and content of carbonate, and crystal morphology. It was observed that the transformation was faster with higher hydrothermal temperature. However, higher hydrothermal temperature caused de-carbonation phenomena which resulted in the lack of carbonate ions of the product. Moreover, the higher the percentage of Ca-hydroxide added to gypsum, caused the complete transformation of gypsum into CHA to be slower. These findings have been applied to the standard fabrication procedure of carbonate apatite, which in turn will allow scaling up process, and will be provided for biomedical purposes for the Indonesian community.
- C. J. Kirkpatrick, S. Fuchs, K. Peters, C. Brochhausen, M. I. Hermanns and R. E. Unger, “Visions for Regenerative Medicine: Interface between Scientific Fact and Science Fiction,” Artif Organs, Vol. 30, 2006, pp. 822-827.
- W. L. Murphy and D. J. Mooney, “Bioinspired Growth of Crystalline Carbonate Apatite on Biodegradable Polymer Substrata,” Journal of the American Chemical Society, Vol. 124, 2002, pp. 1910-1917.
- S. C. G. Leeuwenburgh, I. D. Ana and J. Jansen, “Sodium Citrate as an Effective Dispersant for Synthesis of Inorganic-Organic Composites with Nanodispersed Mineral Phase,” Acta Biomaterialia, 2009.
- J. V. Rau, S. N. Cesaro, D. Ferro, S. M. Barinov and J. V. Fadeeva, “FTIR Study of Carbonate Loss from Carbonated Apatites in Wide Temperature Range,” Journal of Biomedical Materials Research Part B: Applied Biomaterials, Vol. 71B, No. 2, 2004, pp. 441-447.
- A. A. Baig, J. L. Fox, J. Su, Z. Wang, M. Otsuka, W. I. Higuchi and R. Z. Legeros, “Effect of Carbonate Content and Crystallinity on the Metastable Equilibrium Solubility Behavior of Carbonate Apatite,” Journal of Colloid and Interface Science, Vol. 179, 1996, pp. 608-617.
- R. Tang, Z. J. Henneman and G. H. Nancollas, “Size Effects in the Dissolution of Hydroxyapatite an Understanding Biological Demineralization,” Journal of Crystal Growth, Vol. 249, 2003, p. 614.
- I. Y. Rieters, E. A. P. D. Maeyer and R. M. H Verbeeck, “Conversion of Octacalcium Phosphate in Calcium Phosphate Cements,” Inorganic Chemistry, Vol. 35, 1996, p. 5791.
- H. R. Wenk, F. Heidelbach, “Crystal Alignment of Calcium Phosphate in Bone and Calcified Tendon,” Bone, Vol. 24, No. 4, 1999, pp. 361-369.
- E. Landi, A. Tampieri, G. Celotti, R. Langenati, M. Sandri, S. Sprio, “Influence of Synthesis and Sintering Parameters on the Characteristics of Calcium Phosphate,” Biomaterials, Vol. 26, 2005, p. 2835.
- J. Barralet, S. Best and W. Bonfield, “Carbonate Substitution in Precipitated Hydroxyapatite: An Investigation into Effects of Reaction Temperature and Bicarbonate Ion Concentration,” Journal of Biomedical Materials Research, Vol. 41, No. 1, 1998, p. 79.
- J. Barralet, M. Akao, H. Aoki, “Dissolution of Dense Carbonate Apatite Subcutaneously Implanted in Wistar Rats,” Journal of Biomedical Materials Research, Vol. 49, No. 2, 2000, pp. 176-182.
- W. L. Suchanek, P. Shuk, K. Byrappa, R. E. Riman, K. S. Tenhuisen, V. F. Janas, “Mechanochemical-Hydrothermal Synthesis of Carbonated Apatite Powders at Room Temperature,” Biomaterials, Vol. 23, 2002, pp. 699-710.