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Rapid Determination of Sexually Transmitted Infections by Real-time Polymerase Chain Reaction Using Microchip Analyzer
Department of chemistry, Saint Petersburg State University, Saint Petersburg, Russia Saint Petersburg, Russia
Department of chemistry, Saint Petersburg State University, Saint Petersburg, Russia
Department of chemistry, Saint Petersburg State University, Saint Petersburg, Russia Saint Petersburg, Russia
Lumex Ltd., Saint Petersburg, Russia
Lumex Ltd. Saint Petersburg, Russia
Lumex Ltd., Saint Petersburg, Russia
Lumex Ltd., Saint Petersburg, Russia
Lumex Ltd., Saint Petersburg, Russia
- 1 Department of chemistry, Saint Petersburg State University, Saint Petersburg, Russia Saint Petersburg, Russia
- 2 Department of chemistry, Saint Petersburg State University, Saint Petersburg, Russia
- 3 Department of chemistry, Saint Petersburg State University, Saint Petersburg, Russia Saint Petersburg, Russia
- 4 Lumex Ltd., Saint Petersburg, Russia
- 5 Lumex Ltd. Saint Petersburg, Russia
- 6 Lumex Ltd., Saint Petersburg, Russia
- 7 Lumex Ltd., Saint Petersburg, Russia
- 8 Lumex Ltd., Saint Petersburg, Russia
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Abstract
Development of non expensive and time-saving techniques based on the polymerase chain reaction (PCR) is of great importance for modern diagnostics. We considered a new approach for PCR determination of a variety of sexually transmitted infections using microchip analyzer “AriaDNA”, which had been tested using clinical samples in several medical institutions of St. Petersburg (Russia). The use of microchips containing lyophilized PCR reagents allows reducing significantly time of analysis and the number of manipulations thus preventing possible sample contamination.
KeywordsPolymerase Chain ReactionSexually Transmitted InfectionsMicrochip
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