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Mutations in Caprine DGAT1 and STAT5A Genes were Associated with Milk Production Traits
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
college of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
- 1 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
- 2 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
- 3 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
- 4 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
- 5 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
- 6 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
- 7 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
- 8 college of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi P.R. China
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Abstract
In this study, polymorphisms of the DGAT1 andSTAT5A genes were detected in 528 individuals from Xinong Saanen and Guanzhong goat breeds by PCR-RFLP, PCR-SSCP and DNA sequencing methods. Three allelic variants were identified: DQ380250: g.407_408insC, AJ237937: g.6798C>T and g.6852C>T in both breeds. At g.407_408insC locus, the frequencies of C 1 allele were 0.79–0.85, and frequencies of C 2 allele were 0.21–0.15. At g.6852C>T locus, frequencies of C 3 allele were 0.70–0.72, and frequencies of T 3 allele were 0.30–0.28. Compared with goats with C 1 C 1 and C 3 C 3 , those with C 1 C 2 and C 3 T 3 genotypes had significant effects on milk yield and fat percentage (P<0.05), respectively. The result showed that does with C 1 C 1 C 3 T 3 and C 1 C 2 C 3 T 3 had higher milk yield than those with C 1 C 2 C 3 C 3 (P < 0.05). In addition, the combined effect of C 1 C 2 C 3 C 3 on milk fat percentage was the highest in comparison with other combination genotypes (P<0.05).
KeywordsDairy GoatMilk Production TraitsFat PercentagePedigree
- X.P. An, S.G. Song, J.X. Hou, C.M. Zhu, J.X. Peng, X.Q. Liu, et al., “Polymorphism identification in goat DGAT2 gene and association analysis with milk yield and fat percentage,” Small Ruminant Research, vol. 100, pp. 107-112, 2011.
- J.C. Dekkers, “Commercial application of marker- and gene-assisted selection in livestock: strategies and lessons,” Journal of Animal Science, vol. 82, pp. E313-328, 2004.
- I. Parmentier, D. Portetellea, N. Gengler, A. Prandic, C. Bertozzia, L. Vleuricka, et al., “Candidate gene markers associated with somatotropic axis and milk selection,” Domestic Animal Endocrinology, vol. 17, pp. 139-148, 1999.
- P. Hatzopoulos, G. Banilas, M. Karampelias, I. Makariti, and A. Kourti, “The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1,” Journal of Experimental Botany, val. 62, pp. 521-532, 2011.
- K. Giannoulia, K. Haralampidis, Z. Poghosyan, D.J. Murphy, and P. Hatzopoulos, “Differential expression of diacylglycerol acyltransferase (DGAT) genes in olive tissues,” Biochemical Society Transactions, vol. 28, pp. 695-697, 2000.
- S. Cases, S.J. Stone, P. Zhou, E. Yen, B. Tow, K.D. Lardizabal, et al., “Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase and related family members,” Journal of Biological Chemistry, vol. 276, pp. 38870-38876, 2001.
- B. Grisart, W. Coppieters, F. Farnir, L. Karim, C. Ford, P. Berzi, et al., “Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition,” Genome Research, vol. 12, pp. 222-231, 2002.
- A. Winter, W. Kramer, F.A.O. Werner, S. Kollers, S. Kata, G. Durstewitz, et al., “Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA : diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content,” Proceedings of the National Academy of Sciences, vol. 99, pp. 9300-9305, 2002.
- J.E. Darnell, I.M. Kerr, and G.R.Stark, “Jak-Stat pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins,” Science, vol. 264, pp. 1415-1421, 1994.
- H. Wakao, F. Gouilleux, and B.Groner, “Mammary-gland factor (Mgf) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response,” Embo Journal, vol. 13, pp. 2182-2191, 1994.