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Leptin Receptor in Ram Epididymal Spermatozoa
Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
- 1 Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
- 2 Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
- 3 Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
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Abstract
This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then they were purified. Specific bands (98 and 308 bp) for Ob-R and Ob-Rb were detected after RT-PCR in both epididymal spermatozoa and placental cotyledon. We may conclude that Ob-R and Ob-Rb mRNA are present in ram epididymal spermatozoa and leptin perhaps exerts physiological effects, as already demonstrated in human.
KeywordsEpididymal SpermatozoamRNAOb-ROb-Rb
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