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Fast Determination of Vitamin B<sub>2</sub> Based on Molecularly Imprinted Electrochemical Sensor
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
- 1 Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
- 2 Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
- 3 Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
- 4 Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
- 5 Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
- 6 Key Laboratory of Industrial Microbiology, Ministry of Education, College of Bioengineering, Tianjin Key Laboratory of Industry Microbiology, College of Bioengineering, Tianjin University of Science & Technology, TUST, Tianjin, P.R.China
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Abstract
Under the condition of weak acidity of pH 5.2, a sensitive vitamin B 2 electrochemical sensor based on molecularly imprinted nonconducting polymer of o-aminophenol by potentiostatic polymerization in the presence of template(vitamin B 2 ) on a glassy carbon electrode was prepared, and its performance was studied. The sensor exhibited good sensitivity and selectivity to VB 2 . The detection limit went down to 2.3851nM, and a linear relationship between the current incremental and the concentration was found in the range of 10～120nM. And the sensor could use in detection of VB2 real sample for a long time and show good reproducibility. The average recovery rate to VB2 was 98.41%.
KeywordsVitamin B2Molecularly Imprinted TechniquePotentiostatic PolymerizationO-aminophenolDrug Determination
- [1] A. Niazi, A. Yazdanipour, J. Ghasemi and A. Abbasi, “Determination of riboflavin in human plasma by excitation-emission matrix fluorescence and multi-way analysis,” J. Chin. Chem Soc., vol. 53, pp. 503-510, 2006.
- L. Hu, “Determination of riboflavin by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection and study on a new reflction detection for capillary electrophoresis,”. Chengdu: college of chemistry, Sichuan University, 2007.
- M. Wang, L. X. Zhao, M.L.Liu, J.M. Lin, “Determination of fiboflavin by enhancing the chemiluminescence intensity of peroxomonosulfate-cobalt(Ⅱ) system”, Spectrochimica Acta Part A, vol. 66, pp. 1222-1227. 2007.
- Q.Y. Li, H. Li, “Determination of vitamin B2 based on Luminol-Cu(Ⅱ) system,” Chinese Journal of Spectroscopy Laboratory, vol. 28, No. 4, pp. 1986-1989, July 2011.
- L.J. Yuan, D.B. Chen, “Determination of vitamin B1, vitamin B2 in four vitamin glucose calcium particles for children by HPLC,” Journal of Yichun College, vol. 30, No. 4, pp. 89-90, August 2008.
- X. Y. Bi, Z. L. Zhao, N. H. Fu, X. M. Li, L. Li, G. P. Zhang, “HPLC determination of the content of the water-soluble vitamins in ferrous sulfate and vitamin sustained-release tablets,” Pharm. J Chin. PLA, vol. 23, No. 5, pp. 389-391, October 2007.
- A. Gliszczynska-Swiglo, A. Kozio?owa, “Chromatographic determination of riboflavin and its derivatives in food”, Journal of Chromatography A, vol. 881, pp. 285-297, 2000.
- R. M. Kotkasr, P. B. Desai, A. K. Srivastava, “Behavior of riboflavin on plain carbon paste and aza macrocycles based chemically modified electrodes,” Sensors and Acuators B, vol. 124, pp. 90-98, 2007.
- S. L. Bhattar, G. B. Kolekar, S. R. Patil, “Fluorescence resonance energy transfer between perylene and riboflavin in micellar solution and analytical application on determination of vitamin B2,” Journal of Luminescence, vol. 128, pp. 306-310, 2008.
- Y. Y Su, X. H. Li, “Indirect determination of riboflavin in pharmaceuticals by flame atomic absorption spectrometry,” Chemical Research and Application, vol. 23, No. 3, pp. 364-366, March 2011.
- A. K. Su, C. H. Lin, “Determination of riboflavin in urine by capillary electrophoresis-blue light emitting diode-induced fluorescence detection combined with a stacking technique,” Journal of Chromatography B, vol. 785, pp. 39-46, 2003.
- M. Z. ángel Ríos, “Supercritical fluid extraction as on-line clean-up technique for determination of riboflavin vitamins in food samples by capillary electrophoresis with fluorimetric detection,” Electrophoresis, vol. 29, pp. 3213-3219, 2008.