Research ArticleOpen AccessGoogle Scholar indexed
Functional analysis of OAT gene in Aspergillus Niger
MOE key laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China
MOE key laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China
MOE key laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China
- 1 MOE key laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China
- 2 MOE key laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China
- 3 MOE key laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China
Copy link · social · email
Abstract
Mitochondrial oxaloacetate transporter protein encoded by OAT gene transports oxaloacetate from cytoplasm into mitochondria. To investigate the primary effects of OAT gene on relative metabolism in Aspergillus niger , a oat-deleted mutant was derived from wild-type A. niger ATCC1015 using the double-crossover chromosome replacement technique. Then batch fermentation was performed to evaluate the mutant. Compared with the wild type, the mutant showed lower organic acids production, with the experimental data that the production of pyruvate and 2-ketoglutarate decreased by 31.6% and 35.7%, respectively, and by contrast, the mutant showed higher glycerol formation. The results suggest that OAT gene plays significant roles on metabolism in A. niger .
KeywordsAspergillus NigerOxaloacetate TransporterOrganic AcidsGlycerol Formation
- S. Meijer, W. A. de Jongh, L. Olsson and J. Nielsen, “Physiological characterisation of acuB deletion in Aspergillus niger,” Applied Microbiology and Biotechnology, vol. 84, pp. 157–167, 2009.
- W.A. de Jongh, J. Nielsen, “Enhanced citrate production through gene insertion in Aspergillus niger,” Metabolic Engineering, vol.10, pp. 87-96, 2008.
- H. J Pel, J. H de Winde, D. B Archer, P. S Dyer, G. Hofmann, P. J Schaap, et al, “Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88,” Nature Biotechnology, vol. 25, pp. 221-231, 2007.
- Baker SE, “Aspergillus niger genomics: past, present and into the future,” Medical Mycology, vol. 44, pp. 17–21, 2006.
- V. Meyer, M. Arentshorst, A. El-Ghezal, A. C. Drews, R. Kooistra, van den Hondel CAMJ, et al, “Highly efficient gene targeting in the Aspergillus niger kusA mutant,” Journal of Biotechnology, vol. 128, pp. 770–775, 2007.
- Z. Jinxiang, M. Zhihui, X. Wei, L. Ying, T. Guomin, W. Aoquan, et al, “Ku80 gene is related to non-homologous end-joining and genome stability in Aspergillus niger,” Current Microbiology, vol. 62, pp. 1342–1346, 2011.
- N. D. S. P. Carvalho, M. Arentshorst, M. J. Kwon, V. Meyer, A. hur, F. J. Ram, “Expanding the ku70 toolbox for filamentous fungi: establishment of complementation vectors and recipient strains for advanced gene analyses,” Applied Microbiology and Biotechnology, vol. 87, pp. 1463–1473, 2010.
- M. Yigitoglu, “Production of citric acid by fungi,” Journal of Islamic Academy of Sciences, vol. 5, pp. 100-106, 1992.
- M. J. A. de Groot, P. Bundock, P. J. J. Hooykaas, A. G. M. Beigersbergen, “Agrobacterium tumefaciens-mediated transformation of filamentous fungi,” Nature Biotechnology, vol. 16, pp. 839-842, 1998.
- C. B Michielse, P. J J Hooykaas, C. A M J J van den Hondel, A. F J Ram, “Agrobacterium-mediated transformation of the filamentous fungus Aspergillus awamori,” Nature Protocols, vol. 3, pp. 1671-1678, 2008.
- H. Liu, A. Suresh, F. S Willard, D. P Siderovski, S. Lu and N. I Naqvi, “Rgs1 regulates multiple Ga subunits in Magnaporthe pathogenesis, asexual growth and thigmotropism,” The EMBO Journal, vol. 26, pp. 690–700, 2007.
- K. Kapoor，K. Chaudhary，P. Tauro, “Citric acid,” Prescott and Dunn's Industrial Microbiology， vol. 4, pp. 709-742, 1982.