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The omp2 Gene of HPS-type Bacteria Cloning and Sequence Analysis Isolates from Sichuan Province
Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
Tibet Changdu animal health and phytosanitary supervision, TCAHPS, Changdu, China
- 1 Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
- 2 Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
- 3 Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
- 4 Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
- 5 Sichuan Agricultural University, Sichuan Laboratory of Animal Disease and Human Health, SLADHH, Ya'an ,China
- 6 Tibet Changdu animal health and phytosanitary supervision, TCAHPS, Changdu, China
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Abstract
In order to compare the homology and antigen of Haemophilus parasuis (HPS)4 outer membrane protein P2(omp2), we design a test with specific primers, using PCR amplification of isolates of Haemophilus parasuis from Sichuan Province(HP Sch2010), ompP2 gene will be cloned into the pGM-T vector, and transformed into E. coli DH5α. Identified by PCR and sequencing and analysis, the sequencing results showed that the published 4 HPS SW124 strains omp2 gene (1077bp), compared with the amplified 1086bp purpose fragment(containing omp2 genes), is relatively stable, with the nucleotide homology level 97% and amino acid homology level of 92.5%. The variable regions are mainly concentrated in the three base sequences: 40-65,110-156,180-202.
KeywordsHaemophilus parasuisvirulence genesomp2
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