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Isolation and Characterization of an L-Amino Acid Oxidase-Producing Marine Bacterium
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, China
- 1 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
- 2 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
- 3 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
- 4 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China
- 5 College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, China
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Abstract
One marine bacterial strain, R3, has been newly isolated from the intertidal zone of Dinghai sea area. Measurements of a-keto acids and H2O2existing in fermentation supernatant were carried out to show that R3 can produce L-amino acid oxidase (LAAO) with a broad substrate specificity. Physiological and biochemical analysis showed that it can grow great at the conditions with sodium chloride concentration of 1.5%-3%, temperature of 15?C -35?C and pH of 6-7. In addition, molecular identification of 16S rDNA was performed to show that R3 was proximal toPseudoalteromonasspp. with the highest identity of 98.5% toPseudoalteromonasrubra. Therefore, it was designated asPseudoalteromonassp. R3. Further studies are required to arrive at a better understanding of this LAAO and secure an application.
KeywordsLAAOPseudoalteromonasPhysiological PropertyBiochemical PropertyIdentification
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