Detection of Campylobacter sp. from Poultry Feces in Ouagadougou, Burkina Faso

Abstract

Background: Campylobacter contamination in poultry and poultry product has been reported worldwide. The present study aims to determine the prevalence of Campylobacter in poultry feces using selective enrichment Bolton broth and multiplex PCR. Method: Two methods were used in this study : the first was direct plating of poultry feces into mCCDA agar plates. The second , three antibiotics were used at different concentrations to add in Bolton broth supplemented. These antibiotics were Rifampicin (Oxoid, Nepean, Ontario) with 10 mg/L, colistin (Oxoid, Nepean, Ontario) with 1 mg/mL and 2 mg/mL; trimethoprim (Oxoid, Nepean, Ontario) with 10 mg/L. The colonies with typical campylobacter morphology on blood agar (little, red and ring colonies) were further identified to the species level by multiplex polymerase chain reaction (PCR). Results: The addition of colistin (2 mg/mL) to the Bolton broth with selective supplements enhanced the selective isolation of Campylobacter strains. Out of the 52 feces samples, 18 (34.61%) were positive for campylobacter and direct plating on mCCDA 11 (21.15%) campylobacter strains (p < 0.05). The PCR results have shown that 17 (94.45%) of the campylobacter strains detected belonged to Campylobacter coli and 1 (5.55%) strain to Campylobacter jejuni . Conclusion: Although it i s known to be difficult to isolate Campylobacter from animal feces samples, this study show s that antibiotic selective pressure improves the isolation efficiency of Campylobacter from poultry feces.