Advanced Glycation End Products (AGEs) have been associated as a possible cause in inflammation-mediated chronic diseases such as diabetes, Alzhei mer’s and cardiovascular disorders. Thus, inhibition of AGE formation repre sents a prospective therapeutic target for the prevention and treatment of these complications. This study investigated the individual and combined effect s of dietary ingredients, spices, on lowering AGEs formation in meat pat ties. In the study, Carboxymethyllysine (CML), a well-investigated AGE is used as a marker for AGEs and malondialdehyde (MDA) as an indicator for lipid peroxidation. Nine spices were selected based on their ability to inhibit the formation of AGEs at different stages of Maillard reactions. Individually, all the 9 selected spices significantly inhibited the formation of AGEs. Among the 33 combinations of spices, 26 combinations significantly inhibited the formation of AGEs. The highest reduction (84%) was found by the combination of Black Pepper - Rose - Cumin. The individual spices failed to significantly lower the MDA concentration ; however, all 33 combinations were able to signifi cantly reduce MDA concentration . The results of this study showed that spices when supplemented in combinations are more effective in inhibiting the formation of AGEs and in decreasing MDA concentration in meat patties.
Hodge, J.E. (1953) Dehydrated Foods, Chemistry of Browning Reactions in Model Systems. Journal of Agricultural and Food Chemistry, 1, 928-943. https://doi.org/10.1021/jf60015a004
Powrie, W.D., Wu, C.H. and Molund, V.P. (1986) Browning Reaction Systems as Sources of Mutagens and Antimutagens. Environmental Health Perspectives, 67, 47-54. https://doi.org/10.1289/ehp.866747
Wu, C.H., Huang, S.-M., Lin, J.-A. and Yen, G.-C. (2011) Inhibition of Advanced Glycation End Products Formation by Foodstuffs. Food & Function, 2, 224-234. https://doi.org/10.1039/c1fo10026b
Bastos, D.M., Siguemoto, E. and Sefora, M. (2012) Maillard Reactions Products in Processed Food: Pros and Cons. In: Valdez, B., Ed., Food Industrial Processes: Method and Equipments, IntechOpen, London, 281-300.
Vistoli, G., De Maddis, D., Cipak, A., Zarkovic, N., Carini, M. and Aldini, G. (2013) Advanced Glycoxidation and Lipoxidation End Products (AGEs and ALEs): An Overview of Their Mechanism of Formation. Free Radical Research, 47, 3-27. https://doi.org/10.3109/10715762.2013.815348
Chuyen, N.V. (2006) Toxicity of the AGEs Generated from the Maillard Reaction: on the Relationship of Food-AGEs and Biological-AGEs. Molecular Nutrition & Food Research, 50, 1140-1149. https://doi.org/10.1002/mnfr.200600144
Srey, C., Hull, G.L.J., Connolly, L., Elliott, C.T., del Castillo, M.D. and Ames, J.M. (2010) Effect of Inhibitor Compounds on Nε-(Carboxymethyl)lysine (CML) and Nε-(Carboxyethyl)lysine (CEL) Formation in Model Foods. Journal of Agricultural and Food Chemistry, 58, 12036-12041. https://doi.org/10.1021/jf103353e
Nass, N., Bartling, B., Santos, A.N., Scheubel, R.J., Börgermann, J., Silber, R.E., et al. (2007) Advanced Glycation End Products, Diabetes and Ageing. Zeitschrift für Gerontologie und Geriatrie, 40, 349-356. https://doi.org/10.1007/s00391-007-0484-9
Poulsen, M.W., Hedegaard, R.V., Andersen, J.M., de Courten, B., Bügel, S., Nielsen J., et al. (2013) Advanced Glycation End Products in Food and Their Effects on Health. Food and Chemical Toxicology, 60, 10-37. https://doi.org/10.1016/j.fct.2013.06.052
Reddy, V.P. and Beyaz, A. (2006) Inhibitors of the Maillard Reaction and AGE Breakers as Therapeutics for Multiple Diseases. Drug Discovery Today, 11, 646-654. https://doi.org/10.1016/j.drudis.2006.05.016
Ahmad, M.S. and Ahmed, N. (2006) Antiglycation Properties of Aged Garlic Extract: Possible Role in Prevention of Diabetic Complications. The Journal of Nutrition, 136, 796S-799S. https://doi.org/10.1093/jn/136.3.796S
Dearlove, R.P., Greenspan, P., Hartle, D.K., Swanson, R.B. and Hargrove, J.L. (2008) Inhibition of Protein Glycation by Extracts of Culinary Herbs and Spices. Journal of Medicinal Food, 11, 275-281. https://doi.org/10.1089/jmf.2007.536
Ho, S.-C., Chang, P.-W., Tong, H.-T. and Yu, P.-Y. (2014) Inhibition of Fluorescent Advanced Glycation End-Products and N-Carboxymethyllysine Formation by Several Floral Herbal Infusions. International Journal of Food Properties, 17, 617-628. https://doi.org/10.1080/10942912.2012.654566
Hsieh, C.L., Peng, C.H., Chyau, C.C., Lin, Y.C., Wang, H.E. and Peng, R.Y. (2007) Low-Density Lipoprotein, Collagen, and Thrombin Models Reveal that Rosemarinus officinalis L. Exhibits Potent Antiglycative Effects. Journal of Agricultural and Food Chemistry, 55, 2884-2891. https://doi.org/10.1021/jf0631833
Joglekar, M.M., Panaskar, S.N. and Arvindekar, A.U. (2014) Inhibition of Advanced Glycation End Product Formation by Cymene—A Common Food Constituent. Journal of Functional Foods, 6, 107-115. https://doi.org/10.1016/j.jff.2013.09.024
Morimitsu, Y., Yoshida, K., Esaki, S. and Hirota, A. (1995) Protein Glycation Inhibitors from Thyme (Thymus vulgaris). Bioscience, Biotechnology, and Biochemistry, 59, 2018-2021. https://doi.org/10.1271/bbb.59.2018
Peng, X., Cheng, K.W., Ma, J., Chen, B., Ho, C.T., Lo, C., et al. (2008) Cinnamon Bark Proanthocyanidins as Reactive Carbonyl Scavengers to Prevent the Formation of Advanced Glycation Endproducts. Journal of Agricultural and Food Chemistry, 56, 1907-1911. https://doi.org/10.1021/jf073065v
Bosca, A.R., Gutierrez, M.A.C., Soler, A., Puerta, C., Diez, A., Quintanilla, E., et al. (1997) Effects of the Antioxidant Turmeric on Lipoprotein Peroxides: Implications for the Prevention of Atherosclerosis. Age, 20, 165-168. https://doi.org/10.1007/s11357-997-0015-z
Sajithlal, G.B., Chithra, P. and Chandrakasan, G. (1998) Effect of Curcumin on the Advanced Glycation and Cross-Linking of Collagen in Diabetic Rats. Biochemical Pharmacology, 56, 1607-1614. https://doi.org/10.1016/S0006-2952(98)00237-8
Suantawee, T., Wesarachanon, K., Anantsuphasak, K., Daenphetploy, T., Thien-Ngern, S., Thilavech, T., et al. (2015) Protein Glycation Inhibitory Activity and Antioxidant Capacity of Clove Extract. Journal of Food Science and Technology, 52, 3843-3850. https://doi.org/10.1007/s13197-014-1452-1
He, J., Zeng, M., Zheng, Z., He, Z. and Chen, J. (2014) Simultaneous Determination of Nε-(Carboxymethyl)lysine and Nε-(Carboxyethyl)lysine in Cereal Foods by LCMS/MS. European Food Research and Technology, 238, 367-374. https://doi.org/10.1007/s00217-013-2085-8
Tareke, E., Forslund, A., Lindh, C.H., Fahlgren, C. and Östman, E. (2013) Isotope Dilution ESI-LC-MS/MS for Quantification of Free and Total Nε-(1-Carboxymethyl)-L-Lysine and Free Nε-(1-Carboxyethyl)-L-Lysine: Comparison of Total Nε-(1-Carboxymethyl)-L-Lysine Levels Measured with New Method to ELISA Assay in Gruel Samples. Food Chemistry, 141, 4253-4259. https://doi.org/10.1016/j.foodchem.2013.07.003
Botsoglou, N.A., Fletouris, D.J., Papageorgiou, G.E., Vassilopoulos, V.N., Mantis, A.J. and Trakatellis, A.G. (1994) Rapid, Sensitive, and Specific Thiobarbituric Acid Method for Measuring Lipid Peroxidation in Animal Tissue, Food, and Feedstuff Samples. Journal of Agricultural and Food Chemistry, 42, 1931-1937. https://doi.org/10.1021/jf00045a019
Jin, S. and Cho, K.H. (2011) Water Extracts of Cinnamon and Clove Exhibits Potent Inhibition of Protein Glycation and Anti-Atherosclerotic Activity In Vitro and In Vivo Hypolipidemic Activity in Zebrafish. Food and Chemical Toxicology, 49, 1521-1529. https://doi.org/10.1016/j.fct.2011.03.043
Zhang, Y., Henning, S.M., Lee, R.P., Huang, J., Zerlin, A., Li, Z., et al. (2015) Turmeric and Black Pepper Spices Decrease Lipid Peroxidation in Meat Patties during Cooking. International Journal of Food Sciences and Nutrition, 66, 260-265. https://doi.org/10.3109/09637486.2014.1000837