Phytochemical Study and Evaluation of the Antiradical Activity of Extracts of Oleaginous Seeds of <i>Panda oleosa</i> and <i>Isolona hexaloba</i> from Gabon — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Phytochemical Study and Evaluation of the Antiradical Activity of Extracts of Oleaginous Seeds of <i>Panda oleosa</i> and <i>Isolona hexaloba</i> from Gabon
Laboratory of Chemistry-Biochemistry of the Faculty of Medicine, University of Health Sciences, Libreville, Gabon
,
Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA), National Center for Scientific and Technical Research (CENAREST), Department of Phytochemistry, Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Département de Phytochimie, Libreville, Gabon
,
Ecole Normale Supérieure, Department of Physical Sciences, Laboratoire Pluridisciplinaire des Sciences (LAPLUS), Libreville, Gabon
,
Ecole Normale Supérieure, Department of Physical Sciences, Laboratoire Pluridisciplinaire des Sciences (LAPLUS), Libreville, Gabon
,
Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA), National Center for Scientific and Technical Research (CENAREST), Department of Phytochemistry, Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Département de Phytochimie, Libreville, Gabon
,
Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA), National Center for Scientific and Technical Research (CENAREST), Department of Phytochemistry, Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Département de Phytochimie, Libreville, Gabon
,
Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA), National Center for Scientific and Technical Research (CENAREST), Department of Phytochemistry, Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Département de Phytochimie, Libreville, Gabon
1 Laboratory of Chemistry-Biochemistry of the Faculty of Medicine, University of Health Sciences, Libreville, Gabon
2 Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA), National Center for Scientific and Technical Research (CENAREST), Department of Phytochemistry, Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Département de Phytochimie, Libreville, Gabon
3 Ecole Normale Supérieure, Department of Physical Sciences, Laboratoire Pluridisciplinaire des Sciences (LAPLUS), Libreville, Gabon
4 Ecole Normale Supérieure, Department of Physical Sciences, Laboratoire Pluridisciplinaire des Sciences (LAPLUS), Libreville, Gabon
5 Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA), National Center for Scientific and Technical Research (CENAREST), Department of Phytochemistry, Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Département de Phytochimie, Libreville, Gabon
6 Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA), National Center for Scientific and Technical Research (CENAREST), Department of Phytochemistry, Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Département de Phytochimie, Libreville, Gabon
7 Institute of de Pharmacopoeia and Traditional Medicine (IPHAMETRA), National Center for Scientific and Technical Research (CENAREST), Department of Phytochemistry, Libreville-Gabon Pharmacopée et Médecine Traditionnelle (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Département de Phytochimie, Libreville, Gabon
Our study focused on phytochemical tests and evaluation of the anti-free radical activity of seed extracts of two oleaginous plants from Gabon used in traditional medicine or as condiments: Panda oleosa and Isolona hexaloba . The extraction was carried out by maceration with solvents of increasing polarity: cyclohexane, trichloroethylene, acetone, ethanol and finally distilled water. The total yields of the extracts are about 69.50% for Panda oleosa and 34.28% for Isolona hexaloba . The phytochemical tests carried out on the extracts of the seeds of Panda and Isolona highlight in both seeds the presence of alkaloids, polyphenols, triterpenes, carotenoids, reducing compounds, flavonoids, total sugars, coumarins, anthraquinones, free quinones, free anthracene derivatives, and terpenoids. Isolona seeds also contain leucoanthocyanins, sterols, cardiac glycosides and saponins. Phytochemical tests revealed the absence of tannins and mucilage in both seeds. The free radical scavenging activity was measured by scavenging the free radical cation of 2,2’-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] (ABTS-+) with gallic acid as the reference antioxidant. The results of the free radical scavenging activity of the aqueous and ethanolic extracts of both seeds showed that the aqueous extracts were more active than the ethanolic extracts. The IC50s of the aqueous and ethanolic extracts of Panda seeds are 40 and 60 μg · mL -1 respectively, and those of the aqueous and ethanolic extracts of Isolona are 37.5 and 95 μg · mL -1 respectively. Gallic acid, the reference antioxidant (IC50 = 0.37 μg · mL -1 ) is about 10 times more active than the aqueous extracts of both seeds, 16 times more active than the ethanolic extract of Panda and 25 times more active than the ethanolic extract of Isolena.
FAO (2010) Evaluation des ressources forestières mondiales. Rome, 377.
Raponda-Walker, A. and Sillans, R. (1961) Les plantes utiles du Gabon: Essai d’inventaire et de concordance des noms vernaculaires et scientifiques des plantes spontanées et introduites. Description des espèces, propriétés, utilisations. Paul Lechevalier. ed. Fondation Raponda-Walker. Sépia. Centre Culturel Saint-Exupéry, 12 rue de Tournon Paris-VIe.
Nziengui, B. (2001) Une recette locale: Alimentation par les plantes; peut-on revaloriser le savoir de nos ancêtres? L’exemple de Panda oleosa. Le Cri du Pangolin, 28, 16.
Bokesch, H.R., McKee, T.C., Cardellina II, J.H. and Boyd, M.R. (1994) Ent-4’-O methylgallocatechin from Panda oleosa. Natural Products Letters, 4, 155-157. https://doi.org/10.1080/10575639408043898
Garcia, J., Massoma, T., Morin, C., Mpondo, T.N. and Nyasse, B. (1993) 4’-O-Methylgallocatechin from Panda oleosa. Phytochemistry, 32, 1626-1628. https://doi.org/10.1016/0031-9422(93)85196-X
Wilks, C. and Issembé, Y. (2000) Les arbres de la Guinée Equatoriale: Guide pratique d’identification: Région continentale. Projet CUREF, Bata, Guinée Equatoriale, 546 p.
Vivien, J. and Faure, J.J. (1985) Arbres des forêts denses d’Afrique Centrale. Agence de Coopération Culturelle et Technique, Paris, 565 p.
Fournet, A., Ferreira, M.-E., Rojas de Arias, A., Schinini, A., Nakayama, H., Torres, S., Sanabria, R., Guinaudeau, H. and Bruneton, J. (1997) The Effect of Bisbenzylisoquinoline Alkaloids on Trypanosoma cruzi Infections in Mice. International Journal of Antimicrobial Agents, 8, 163-170. https://doi.org/10.1016/S0924-8579(97)00373-7
Re, R., Pellegrini, N., Proteggente, A., Pannala, Y.M. and Rice-Evans, C. (1999) Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology & Medicine, 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
Ngou-Milama, E., et al. (2020) Cinétique d’étude de l’activité antioxydante d’un extrait aqueux de calices séchés d’Hibiscus Sabdariffa par piégeage de l’ion radicalaire ABTS +. IJAR, 6, 361-367. https://doi.org/10.22271/allresearch.2020.v6.i11f.7915
Houghton, P. and Raman, A. (1998) Laboratory Handbook for the Fractionation of Natural Extracts. Springer, Berlin. https://doi.org/10.1007/978-1-4615-5809-5_1
Akinjogunla, O.J., Yah, C.S., Eghafona, N.O., Ogbemudia, F.O. (2010) Antibacterial Activity of Leave Extracts of Nymphaea lotus (Nymphaeaceae) on Methicillin Resistant Staphylococcus aureus (MRSA) and Vancomycin Resistant Staphylococcus aureus (VRSA) Isolated Fromclinical Samples. Annals of Biological Research, 1, 174-184.
Phytochemical Screening
Antiradical Activity
ABTS-+
Gallic Acid
Badiaga, M. (2012) Etude ethnobotanique, phytochimique et activités biologiques de Nauclea latifolia Smith, une plante médicinale africaine récoltée au Mali. Thèse, Université de Bamako, Bamako, 30-32.
N’Guessan, J.D., Boni, A.R., Zirihi, G.N. and Djaman, A.J. (2009) Relation entre les activités antioxydantes et les teneurs en polyphénols de six plantes de la pharmacopée ivoirienne. Laboratoire Pharmacodynamie Biochimique, UFR Biosciences, Université de Cocody-Abidjan, Abidjan.
Muanda, F.N. (2010) Identification de polyphénols, évaluation de leur activité antioxydante et étude de leurs propriétés biologiques. Thèse doctorale 3ème cycle en sciences fondamentales et appliquées, Université Paul Verlaine. Met3.France.
Bentabetlasgaa, N. (2015) Etude phytochimique et évaluation des activités biologiques de deux plantes Fredoleiareteoides et Echmimuvalgare de l’ouest algérien. Thèse de doctorat, 20-21.
Harborne, J.B. and Grayer, R.J. (1988) The Anthocyanins. In: Harborne, J.B., Ed., The Flavonoids. Advances in Research since 1980, Springer, Berlin, 1-20. https://doi.org/10.1007/978-1-4899-2913-6_1
Yam, M.F., Ang, L.F., Ameer, O.Z., Salman, I.M., Aziz, H.A. and Asmawi, M.Z. (2009) Anti-Inflammatory and Analgesic Effects of Elephantopus tomentosus Ethanolic Extract. Journal of Acupuncture and Meridian Studies, 2, 280-287. https://doi.org/10.1016/S2005-2901(09)60069-8
Oloyede, O.I. (2005) Chimical Profile of Unripe Pulp of Carica papaya. Pakistan Journal of Nutrition, 4, 379-381. https://doi.org/10.3923/pjn.2005.379.381
Fournet, A. (1979) Plantes médicinales congolaises, Meiocarpidium, limaciopsis. Trav et doc de l’ORSTOM, Paris.
Mengome, L.E., FeuyaTchouga, G.R., Eba, F. and Nsi-Emvo, E. (2009) Antiproliferative Effect of Alcoholic Extracts of Some Gabonese Medicinal Plants on Human Colonic Cancer Cells. African Journal Traditional Complementary Alternative Medicines, 6, 112-117. https://doi.org/10.4314/ajtcam.v6i2.57081
Awor et Samseny, R.R. (2003) Contribution à l’étude phytochimique d’une plante traditionnellement utilisée comme poison d’épreuve au Gabon: Le Strychnos IcajaBaillon (Mbundu), Loganéaceé. Thèse Université de Bamako, Faculté de Médecine, de pharmacie et d’odonto-stomatologie, Mali.
Silou, T. (2014) Corps gras non conventionnels du Bassin du Congo: Caractérisation, biodiversité et qualité. Fats and Oils in the Congo Basin: Characterization, Biodiversity and Quality. OCL, 21, D209. https://doi.org/10.1051/ocl/2013044
Hertog, M.G., Hollman, P.C., Katan, M.B. and Kromhout, D. (1993) Intake of Potentially Anticarcinogenic Flavonoids and Their Determinants in Adults in the Netherlands. Nutrition and Cancer, 20, 21-29. https://doi.org/10.1080/01635589309514267
Middleton Jr., E., Kandaswami, C. and Theoharides, T.C. (2000) The Effects of Plant Flavonoids on Mammalian Cells: Implications for Inflammation, Heart Disease. Pharmacology Review, 512, 673-675.
Leong, L.P. and Shui, G. (2002) An Investigation of Antioxidant Capacity of Fruits in Singapore Markets. Food Chemistry, 76, 69-75. https://doi.org/10.1016/S0308-8146(01)00251-5
Raman, V., La, S. and Saradhi, P. (2012) Antibacterial, Antioxydant Activity and GC-Ms Analysis of Eupatorium odoratum. Asian Journal of Pharmaceutical and Clinical Research, 5, 99-106.
Jaswir, I., Noviendri, D., FitriHasrini, R. and Octavianti, F. (2011) Carotenoids: Sources, Medical Properties and Their Application in Food and Nutraceutical Industry. Journal of Medicinal Plants Research, 5, 7119-7131. https://doi.org/10.5897/JMPRX11.011
Confédération Suisse. Ordonnance du DFI sur les additifs admis dans les denrées alimentaires 2017.
Sadat, L., Cakir-Kiefer, C., Marie-Andrée, N’Negue, M.-A., Gaillard, J.-L., Girardet, J.-M. and Miclo, L. (2011) Isolation and Identification of Antioxidative Peptides from Bovine α-Lactalbumin. International Dairy Journal, 21, 214-221. https://doi.org/10.1016/j.idairyj.2010.11.011
N’Negue ép. Mezui-Mbeng, M.A., Lendoye, E., Nguema Edzang, R.W., Ella Ndong, J.G., Mambimba, C., Ngo-Thiam, E. and Ngou Milama, E. (2021) Evaluation of the Anti-Radical Activity of Methanolic and Aqueous Extracts of Stem, Stem Bark and Leaves of Waltheria indica by Scavenging the Free Radical Cation ABTS. Journal of Medicinal Plants Studies, 9, 122-128. https://doi.org/10.22271/plants.2021.v9.i4b.1320
Biradar, V. (2016) Extraction of Phytochemicals from Local Selected Plants and Their Antibacterial Role. International Journal of Current Microbiology and Applied Sciences, 5, 707-720. https://doi.org/10.20546/ijcmas.2016.511.082
Nga Nnanga, E., Yinyang, J., Baran à Bidias, E., Etame-Loe, G. and Dibong, D.S. (2017) Etude phytochimique et pharmacologique d’Alchorneacordifolia (Schum. & Thonn.) Mull. Arg. et de Mangiferaindica dans le traitement traditionnel de la maladie hémorroidaire. Journal of Applied Biosciences, 109, 10649-10661. https://doi.org/10.4314/jab.v109i1.9
Becker, M.M., Nunes, G.S., Ribeiro, D.B., Silva, F.P.S., Catanante, G. and Mart, J.L. (2019) Determination of the Antioxidant Capacity of Red Fruits by Miniaturized Spectrophotometry Assays. Journal of the Brazilian Chemical Society, 30, 1108-1114. https://doi.org/10.21577/0103-5053.20190003