<i>In Vitro</i> Antioxidant and Free Radical Scavenging Activity of <i>Curcuma longa</i>, <i>Acorus calamus and Camellia sinensis</i> — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
<i>In Vitro</i> Antioxidant and Free Radical Scavenging Activity of <i>Curcuma longa</i>, <i>Acorus calamus and Camellia sinensis</i>
Biochemistry Laboratory, Department of Chemical Engineering, Faculty of Engineering and Technology, Annamalai University, Annamalai Nagar, Tamil Nadu, India
,
Biochemistry Laboratory, Department of Chemical Engineering, Faculty of Engineering and Technology, Annamalai University, Annamalai Nagar, Tamil Nadu, India
,
Biochemistry Laboratory, Department of Chemical Engineering, Faculty of Engineering and Technology, Annamalai University, Annamalai Nagar, Tamil Nadu, India
1 Biochemistry Laboratory, Department of Chemical Engineering, Faculty of Engineering and Technology, Annamalai University, Annamalai Nagar, Tamil Nadu, India
2 Biochemistry Laboratory, Department of Chemical Engineering, Faculty of Engineering and Technology, Annamalai University, Annamalai Nagar, Tamil Nadu, India
3 Biochemistry Laboratory, Department of Chemical Engineering, Faculty of Engineering and Technology, Annamalai University, Annamalai Nagar, Tamil Nadu, India
The purpose of the current study was to determine the total phenolic and flavonoid content and total antioxidant activity of the Curcuma longa , Acorus calamus , and Camellia sinensis ethanolic extracts and their free radical scavenging activity. The study concluded that the Curcuma longa , Acorus calamus , and Camellia sinensis ethanolic extracts have a good source of phenolics, flavonoids, and antioxidant sources in turn which opens the high possibility of the extracts being used as food preservatives. The DPPH assay for scavenging free radicals showed that the IC 50 value was above 123% of Curcuma longa ethanolic extract, 129.9% μg/ml of Acorus calamus ethanolic extract and 25% of Camellia sinensis ethanolic extracts shows very strong inhibition of the free radicals. Thus, comparing the DPPH assay for scavenging free radicals of Curcuma longa , Acorus calamus and Camellia sinensis ethanolic extracts with the positive control ascorbic acid, Curcuma longa and Camellia sinensis ethanolic extracts showed strong inhibition of the free radicals.
Akter, M.J., Khatun, R., Khatune, N.A., Alam, A.K. and Rahman, M.A.A. (2022) In vitro Antioxidant and Free Radical Scavenging Activity of the Bark of Dillenia indica L. Bangladesh Pharmaceutical Journal, 25, 38-43. https://doi.org/10.3329/bpj.v25i1.57839
Huang, T.S., Lee, S.C. and Lin, J.K. (1991) Suppression of c-Jun/AP-1 Activation by an Inhibitor of Tumor Promotion in Mouse Fibroblast Cells. Proceedings of the National Academy of Sciences of the United States of America, 88, 5292-5296. https://doi.org/10.1073/pnas.88.12.5292
Das, R.R., Rahman, M.A., Al-Araby, S.Q., Islam, M.S., Rashid, M.M., Babteen, N.A., Alnajeebi, A.M., Alharbi, H.F.H., Jeandet, P., Rafi, M.K.J., Siddique, T.A., Uddin, M.N. and Zakaria, Z.A. (2021) The Antioxidative Role of Natural Compounds from a Green Coconut Mesocarp Undeniably Contributes to Control Diabetic Complications as Evidenced by the Associated Genes and Biochemical Indexes. Oxidative Medicine and Cellular Longevity, 2021, Article ID: 9711176. https://doi.org/10.1155/2021/9711176
Sharifi-Rad, M., Anil Kumar, N.V., Zucca, P., Varoni, E.M., Dini, L., Panzarini, E., Rajkovic, J., Tsouh Fokou, P.V., Azzini, E., Peluso, I., Prakash Mishra, A., Nigam, M., El Rayess, Y., Beyrouthy, M.E., Polito, L., Iriti, M., Martins, N., Martorell, M., Docea, A.O., Setzer, W.N., Calina, D., Cho, W.C. and Sharifi-Rad, J. (2020) Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases. Frontiers in Physiology, 11, Article No. 694. https://doi.org/10.3389/fphys.2020.00694
Franco, R., Navarro, G. and Martinez-Pinilla, E. (2019) Antioxidants versus Food Antioxidant Additives and Food Preservatives. Antioxidants, 8, Article No. 542. https://doi.org/10.3390/antiox8110542
Alam, A.H.M.K., Hossain, A.S.M.S., Khan, M.A., Kabir, S.R., Reza, M.A., Rahman, M.M., Islam, M.S., Rahman, M.A.A., Rashid, M. and Sadik, M.G. (2016) The Antioxidative Fraction of White Mulberry Induces Apoptosis through Regulation of p53 and NFκB in EAC Cells. PLOS ONE, 11, Article ID: e0167536. https://doi.org/10.1371/journal.pone.0167536
Wijaya, F.L., Suwitono, M.R. and Sulastri, T. (2022) Determination of Antioxidant Activity and Shelf-Life Test of Turmeric (Curcuma longa) Fortified Bread. 8ISC Proceedings: Sciences, 48-54.
Kusuma, F., Wildan Rosyidi, N. and dan Sisi, C. (2019) Manfaat Kunyit (Curcuma longa) dalam Farmasi. INA-Rxiv. Preprint. https://doi.org/10.31227/osf.io/j9a34 https://osf.io/preprints/inarxiv/j9a34/
Azis, A. (2019) Kunyit (Curcuma domestica Val) Sebagai Obat Antipiretik. Jurnal Ilmu Kedokteran dan Kesehatan, 6, 116-120. https://doi.org/10.33024/jikk.v6i2.2265
Retnaningsih, A. (2015) Uji Daya Hambat Rimpang Kunyit (Curcuma domestica Val) dan Rimpang Temulawak (Curcuma xanthorriza roxb) Terhadap Bakteri Salmonella thypi. Jurnal Kesehatan Holistik, 9, 158-160.
Malangngi, L., Sangi, M. and Paendong, D.J. (2012) Penentuan Kandungan Tanin dan Uji Aktivitas Antioksidan Ekstrak Biji Buah Alpukat (Persea americana Mill.). Jurnal MIPA, 1, 5-10. https://doi.org/10.35799/jm.1.1.2012.423
Nuningtyas, Y.F. and Widodo, E. (2018) Increasing Antioxidant Activity of Quail (Cortunix-Cortunix Japonica) Eggs with the Addition of Sweet Flag (Acorus calamus) Powder as a Feed Additive. IOP Conference Series: Earth and Environmental Science, 207, Article ID: 012029. https://doi.org/10.1088/1755-1315/207/1/012029
Heim, K.E., Tagliaferro, A.R. and Bobilya, D.J. (2002) Flavonoid Antioxidant Chemistry Metabolism and Structure-Activity Relationships. The Journal of Nutritional Biochemistry, 13, 572-584. https://doi.org/10.1016/S0955-2863(02)00208-5
Trinovani, E., Prawira-Atmaja, M.I., Kusmiyati, M., Harianto, S. and Maulana, H. (2022) Total Polyphenols and Antioxidant Activities of Green Tea Powder from GMB 7 and GMB 9 Tea Clones. IOP Conference Series: Earth and Environmental Science, 974, Article ID: 012113. https://doi.org/10.1088/1755-1315/974/1/012113
Kochman, J., Jakubczyk, K., Antoniewicz, J., Mruk, H. and Janda, K. (2020) Health Benefits and Chemical Composition of Matcha Green Tea: A Review. Molecules, 26, Article No. 85. https://doi.org/10.3390/molecules26010085
Sakurai, K., Shen, C., Ezaki, Y., Inamura, N., Fukushima, Y., Masuoka, N. and Hisatsune, T. (2020) Effects of Matcha Green Tea Powder on Cognitive Functions of Community-Dwelling Elderly Individuals. Nutrients, 12, Article No. 3639. https://doi.org/10.3390/nu12123639
Ning, J., Hou, G.G., Sun, J., Wan, X. and Dubat, A. (2017) Effect of Green Tea Powder on the Quality Attributes and Antioxidant Activity of Whole-Wheat Flour Pan Bread. LWT - Food Science and Technology, 79, 342-348. https://doi.org/10.1016/j.lwt.2017.01.052
Ainsworth, E.A. and Gillespie, K.M. (2007) Estimation of Total Phenolic Content and Other Oxidation Substrates in Plant Tissues Using Folin-Ciocalteu Reagent. Nature Protocols, 2, 875-877. https://doi.org/10.1038/nprot.2007.102
Nurcholis, W., Nur Sya’bani Putri, D., Husnawati, H., Iis Aisyah, S., Pontjo Priosoeryanto, B. (2021) Total Flavonoid Content and Antioxidant Activity of Ethanol and Ethyl Acetate Extracts from Accessions of Amomum compactum Fruits. Annals of Agricultural Sciences, 66, 58-62. https://doi.org/10.1016/j.aoas.2021.04.001
Blois, M.S. (1958) Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
Sabir, S.M., Zeb, A., Mahmood, M., Abbas, S.R., Ahmad, Z. and Iqbal, N. (2020) Phytochemical Analysis and Biological Activities of Ethanolic Extract of Curcuma longa Rhizome. Brazilian Journal of Biology, 81, 737-740. https://doi.org/10.1590/1519-6984.230628
Sanjay Varshan, M., Gayathri, R., Vishnu Priya, V., Selvaraj, J. and Kavitha, S. (2021) Phytochemical Screening and in Vitro Albumin Denaturation Inhibitory Potential of Methanolic Root Extract of Acorus calamus. Journal of Pharmaceutical Research International, 33, 437-445. https://doi.org/10.9734/jpri/2021/v33i59B34401
Nazliniwaty, Ismanelly Hanum, T. and Laila, L. (2020) Antioxidant Activity Test of Green Tea (Camellia sinensis L. Kuntze) Ethanolic Extract Using DPPH Method. 2018 International Conference of Science, Technology, Engineering, Environmental and Ramification Researches, Medan, 30-31 August 2018, 752-754.