Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis Methods — Oak Academic Publishing
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Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis Methods
Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
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Laboratoire de Mycotoxines, Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
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Laboratoire de Mycotoxines, Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
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Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
,
Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
,
Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
,
Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
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Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
1 Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
2 Laboratoire de Mycotoxines, Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
3 Laboratoire de Mycotoxines, Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
4 Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
5 Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
6 Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
7 Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
8 Laboratoire de Chimie Analytique et Bromatologie, Faculté de Médecine, de Pharmacie et d’Odontologie-Stomatologie, Université Cheikh Anta DIOP, Dakar, Sénégal
Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem related to residues presence in animal origin products. Aflatoxin B1 contamination of poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom, Sangalkam) was studied. A total of 15 samples were collected from Dakar city markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin B1 quantification was performed by high performance liquid chromatography and thin-layer chromatography. HPLC results showed that all samples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom had an aflatoxin B1 level above the maximum limit set by regulations. TLC is a suitable method for aflatoxins detection. However, it was associated with overestimation for aflatoxin B1 quantification. Results suggest that poultry feed represent s a real source of human diet contamination. In addition, HPLC remains the most reliable quantification technique for quality control.
Ladeira, C. (2016) Mycotoxins. In: Viegas, C., Catarina Pinheiro, A., Sabino, R., Viegas, S., Brandao, J. and Veríssimo, C., Eds., Environmental Mycology in Public Health, Academic Press, Cambridge, MA, 343-361. https://doi.org/10.1016/B978-0-12-411471-5.00020-X
Tolosa, J., Rodríguez-Carrasco, Y., Ruiz, M.J. and Vila-Donat, P. (2021) Multi-Mycotoxin Occurrence in Feed, Metabolism and Carry-Over to Animal-Derived Food Products: A Review. Food and Chemical Toxicology, 158, Article ID: 112661. https://doi.org/10.1016/j.fct.2021.112661
Rushing, B.R. and Selim, M.I. (2019) Aflatoxin B1: A Review on Metabolism, Toxicity, Occurrence in Food, Occupational Exposure and Detoxification Methods. Food and Chemical Toxicology, 124, 81-100. https://doi.org/10.1016/j.fct.2018.11.047
Pickova, D., Ostry, V., Toman, J. and Malir, F. (2021) Aflatoxins: History, Significant Milestones, Recent Data on Their Toxicity and Ways to Mitigation. Toxins, 13, Article 399. https://doi.org/10.3390/toxins13060399
Massomo, S.M.S. (2020) Aspergillus flavus and Aflatoxin Contamination in the Maize Value Chain and What Needs to Be Done in Tanzania. Scientific African, 10, e00606. https://doi.org/10.1016/j.sciaf.2020.e00606
Lee, H.S., Nguyen-Viet, H., Lindahl, J., Thanh, H.M., Khanh, T.N., Hien, L.T.T. and Grace, D. (2017) A Survey of Aflatoxin B1 in Maize and Awareness of Aflatoxins in Vietnam. World Mycotoxin Journal, 10, 195-202. https://doi.org/10.3920/WMJ2016.2144
WHO (2018) Aflatoxins. WHO/NHM/FOS/RAM/18.1
Duan, L., Akakpo, J.Y., Ramachandran, A. and Jaeschke, H. (2019) Environmental liver toxins. In: Nriagu, J., Ed., Encyclopedia of Environmental Health, 2nd Edition, Elsevier, Amsterdam, 578-584. https://doi.org/10.1016/B978-0-12-409548-9.11910-4
Mulaudzi, R.B. (2019) Mycotoxins of Medicinal Plants and Human Health. In: Nriagu, J., Ed., Encyclopedia of Environmental Health, 2nd Edition, Elsevier, Amsterdam, 524-530. https://doi.org/10.1016/B978-0-12-409548-9.11532-5
Ishida, Y., Yamasaki, C., Iwanari, H., Yamashita, H., Ogawa, Y., Yanagi, A., Furukawa, S., Kojima, Y., Chayama, K., Kamiie, J. and Tateno, C. (2020) Detection of Acute Toxicity of Aflatoxin B1 to Human Hepatocytes in Vitro and in Vivo Using Chimeric Mice with Humanized Livers. PLOS ONE, 15, e0239540. https://doi.org/10.1371/journal.pone.0239540
Min, L., Fink-Gremmels, J., Li, D., Tong, X., Tang, J., Nan, X., Yu, Z., Chen, W. and Wang, G. (2021) An Overview of Aflatoxin B1 Biotransformation and Aflatoxin M1 Secretion in Lactating Dairy Cows. Animal Nutrition, 7, 42-48. https://doi.org/10.1016/j.aninu.2020.11.002
Zitomer, N., Rybak, M.E., Li, Z., Walters, M.J. and Holman, M.R. (2015) Determination of Aflatoxin B1 in Smokeless Tobacco Products by Use of UHPLC-MS/MS. Journal of Agricultural and Food Chemistry, 63, 9131-9138. https://doi.org/10.1021/acs.jafc.5b02622
Zhao, Y., Wang, Q., Huang, J., Ma, L., Chen, Z. and Wang, F. (2018) Aflatoxin B1 and Sterigmatocystin in Wheat and Wheat Products from Supermarkets in China. Food Additives & Contaminants: Part B, 11, 9-14. https://doi.org/10.1080/19393210.2017.1388295
Nalle, C.L., Supit, M.A.J., Angi, A.H. and Yuliani, N.S. (2021) The Performance, Nutrient Digestibility, Aflatoxin B1 Residue, and Histopathological Changes of Broilers Exposed to Dietary Mycosorb. Tropical Animal Science Journal, 44, 160-172. https://doi.org/10.5398/tasj.2021.44.2.160
Slepchenko, G.B., Gindullina, T.M., Gavrilova, M.A. and Auelbekova, A.Z. (2018) The Simultaneous Voltammetric Determination of Aflatoxins В1 and М1 on a Glassy-Carbon Electrode. Journal of Analytical Methods in Chemistry, 2018, Article ID: 6285623. https://doi.org/10.1155/2018/6285623
Lalah, J.O., Omwoma, S. and Orony, D.A.O. (2020) Aflatoxin B1: Chemistry, Environmental and Diet Sources and Potential Exposure in Human in Kenya. In: Long, X.-D., Ed., Aflatoxin B1 Occurrence, Detection and Toxicological Effects, IntechOpen, Londn.
Bioscope SARL and Experts Associés (2015) Etude de l’impact économique des aflatoxines au Sénégal. Picture Archive Council of America. https://www.aflatoxinpartnership.org/wp-content/uploads/2021/05/v-SN-C-SAAP-Rapport-PACA-_version_finale16-09-2015.pdf
International Organization for Standardization (ISO), ISO 14718:1998, Aliments des animaux—Détermination de la teneur en aflatoxine B1 dans les aliments composés—Méthode par chromatographie liquide à haute performance. 17.
AOAC, AOAC 968.22-2000, Aflatoxins in Peanuts and Peanut Products. AOAC Official Method.
Food and Drug Administration (2000) Guidance for Industry: Action Levels for Poisonous or Deleterious Substances in Human Food and Animal Feed. FDA Guidance Documents. https://www.fda.gov/regulatory-information/search-fda-guidance-documents/guidance-industry-action-levels-poisonous-or-deleterious-substances-human-food-and-animal-feed#afla
Parlement européen et Conseil (2002) Directive 2002/32/CE du Parlement européen et du Conseil du 7 mai 2002 sur les substances indésirables dans les aliments pour animaux. Journal Officiel des Communautés européennes, 30, 5. https://eur-lex.europa.eu/resource.html?uri=cellar:aca28b8c-bf9d-444f-b470-268f71df28fb.0007.02/DOC_1&format=PDF
Ochieng, P.E., Scippo, M.-L., Kemboi, D.C., Croubels, S., Okoth, S., Kang’ethe, E.K., Doupovec, B., Gathumbi, J.K., Lindahl, J.F. and Antonissen, G. (2021) Mycotoxins in Poultry Feed and Feed Ingredients from Sub-Saharan Africa and their Impact on the Production of Broiler and Layer Chickens: A Review. Toxins, 13, Article 633. https://doi.org/10.3390/toxins13090633
Diop, Y.M., Ndiaye, B., Sarr, S.O., Diop, A., Fall, M. and Diouf, A. (2008) Aflatoxines dans les aliments: Recherche et dosage dans les huiles et les pates d’arachide de préparation artisanale. Journal des Sciences pour l’Ingénieur, 10. https://doi.org/10.4314/jspi.v10i1.67904
Diakite, A., Irie, M.G.B., N’Dri, D.K. and Yapo, J.A. (2017) Détermination de la contamination par l’Aflatoxine B1 de la pate d’arachide consommée par la population en Cote d’Ivoire: Intérêt de la Chromatographie sur Couche Mince. International Journal of Biological and Chemical Sciences, 11, 1646-1654. https://doi.org/10.4314/ijbcs.v11i4.19
Guo, Y., Zhao, L., Ma, Q. and Ji, C. (2021) Novel Strategies for Degradation of Aflatoxins in Food and Feed: A Review. Food Research International, 140, Article ID: 109878. https://doi.org/10.1016/j.foodres.2020.109878
Sipos, P., Peles, F., Brassó, D.L., Béri, B., Pusztahelyi, T., Pócsi, I. and Gyori, Z. (2021) Physical and Chemical Methods for Reduction in Aflatoxin Content of Feed and Food. Toxins, 13, Article 204. https://doi.org/10.3390/toxins13030204
Hasanen, F.S., Mohammed, M.M., Mahomud, A.H., Hassan, W.M. and Amro, F.H. (2016) Aflatoxins Residues in Chicken and Turkey Tissues. Benha Veterinary Medical Journal, 31, 130-135. https://doi.org/10.21608/bvmj.2016.31281
Herzallah, S. M. (2013) Aflatoxin B1 Residues in Eggs and Flesh of Laying Hens Fed Aflatoxin B1 Contaminated Diet. American Journal of Agricultural and Biological Sciences, 8, 156-161. https://doi.org/10.3844/ajabssp.2013.156.161
Hakeem, K.R., Oliveira, C.A.F. and Ismail, A., Eds. (2021) Aflatoxins in Food. 1st Edition, Springer, Cham. https://link.springer.com/book/10.1007/978-3-030-85762-2#about
Ali, S., Khan, A.R., Miraj, G., Afridi, S.-R. and Mueen-ud-din, G. (2010) Aflatoxin B1 Contamination in Poultry Feed Available in Local Markets of Peshawar. Pakistan Journal of Biochemistry and Molecular Biology, 43, 37-40.
Anjum, M.A., Khan, S.H., Sahota, A.W. and Sardar, R. (2012) Assessment of Aflatoxin B1 in Commercial Poultry Feed and Feed Ingredients. Journal of Animal and Plant Sciences, 22, 268-272.
Kotinagu, K., Mohanamba, T. and Kumari, L.R. (2015) Assessment of Aflatoxin B1 in Livestock Feed and Feed Ingredients by High-Performance Thin-Layer Chromatography. Veterinary World, 8, 1396-1399. https://doi.org/10.14202/vetworld.2015.1396-1399
Mohammadi, S., Ghahremani, E., Dehestaniathar, S., Zandi, S., Zakariai, A., Mohammadi, M. and Karimi, Z. (2021) Determination of Aflatoxin B1 Concentration in Poultry Feed in the Poultry Farms of Sanandaj Using ELISA Method. Scientific Journal of Kurdistan University of Medical Sciences 25, 49-56. https://doi.org/10.52547/sjku.25.6.49
Sireesha, Y., Keerthana, J.V., Sarathchandra, G. and Velazhahan, R. (2017) Analysis of Aflatoxin B1 and Aflatoxigenic Mold in Commercial Poultry Feeds in Tamil Nadu, India. Journal of Applied and Natural Science, 9, 456-460. https://doi.org/10.31018/jans.v9i1.1213
Kemboi, D.C., Ochieng, P.E., Antonissen, G., Croubels, S., Scippo, M.-L., Okoth, S., Kangethe, E.K., Faas, J., Doupovec, B., Lindahl, J.F. and Gathumbi, J.K. (2020) Multi-Mycotoxin Occurrence in Dairy Cattle and Poultry Feeds and Feed Ingredients from Machakos Town, Kenya. Toxins, 12, Article 762. https://doi.org/10.3390/toxins12120762
Akinmusire, O.O., El-Yuguda, A.-D., Musa, J.A., Oyedele, O.A., Sulyok, M., Somorin, Y.M., Ezekiel, C.N. and Krska, R. (2019) Mycotoxins in Poultry Feed and Feed Ingredients in Nigeria. Mycotoxin Research, 35, 149-155. https://doi.org/10.1007/s12550-018-0337-y
Jayaratne, W.M.S.C., Abeyratne, A.H.M.A.K., De Zoysa, H.K.S., Dissanayake, D.M.R.B.N., Bamunuarachchige, T.C., Waisundara, V.Y. and Chang, S. (2020) Detection and Quantification of Aflatoxin B1 in Corn and Corn-Grown Soils in the District of Anuradhapura, Sri Lanka. Heliyon, 6, e05319. https://doi.org/10.1016/j.heliyon.2020.e05319
Nakavuma, J.L., Kirabo, A., Bogere, P., Nabulime, M.M., Kaaya, A.N. and Gnonlonfin, B. (2020) Awareness of Mycotoxins and Occurrence of Aflatoxins in Poultry Feeds and Feed Ingredients in Selected Regions of Uganda. International Journal of Food Contamination, 7, Article No. 1. https://doi.org/10.1186/s40550-020-00079-2
Kehinde, H.W., Sekoni, A.A., Olugbemi, T.S. and Onimisi, P.A. (2018) Prevalence of Aflatoxin B1 in Some Common Poultry Feed Ingredients and Optimum Inclusion Levels of Mycofix Binder as Feed Additive on Performance of Broiler Chickens. Nigerian Journal of Animal Production, 45, 137-149. https://doi.org/10.51791/njap.v45i2.495
Boli, Z.B.I.A., Camara, F., Toka, D.M., Koussemon, M. and Koffi-Nevry, R. (2018) Validation de la méthode de détermination d’aflatoxine B1 dans les pates d’arachide vendues sur les marchés de la ville d’Abidjan (Cote d’Ivoire). International Journal of Biological and Chemical Sciences, 12, 796-803. https://doi.org/10.4314/ijbcs.v12i2.14
Alshawabkeh, K., Alkhalaileh, N.I., Abdelqader, A., Al-Fataftah, A.-R.A. and Herzallah, S.M. (2015) Occurrence of Aflatoxin B1 in Poultry Feed and Feed Ingredients in Jordan Using ELISA and HPLC. American-Eurasian Journal of Toxicological Sciences, 7, 316-320.
Morrison, D., Ledoux, D., Chester, L. and Samuels, C. (2017) A Limited Survey of Aflatoxins in Poultry Feed and Feed Ingredients in Guyana. Veterinary Sciences, 4, Article 60. https://doi.org/10.3390/vetsci4040060