Semi-Automated Enzymatic Determination of Ethanol in Beverages: Collaborative Study for RIDA®CUBE Ethanol

Abstract

Easy and quick methods to quantify ethanol reliably in beverages are always important. In 2022, the Enzytec TM Liquid Ethanol test kit was approved as AOAC Official Method SM 2017.07 Final Action after a collaborative study was conducted with different beverages such as kombucha, juices, and beer. During set-up of this collaborative test, small sized companies asked to include the RIDA ® CUBE Ethanol/RIDA ® CUBE SCAN device since it is easy to use, suitable for a few samples only and contains the identical reagents as the En zytec TM Liquid system. It is applicable to quantify ethanol in diluted kombucha, fruit juices, and alcohol-free beer samples around 0.5% alcohol-by-volume within 12 min. The overall relative reproducibility standard deviation across a wide concentration range for kombucha, was calculated to be 6.29%. Analysis of juices and beer showed an overall higher variation with an estimated overall RSD(R) value by regression of 14.4%. The data obtained by this collaborative study show that the RIDA ® CUBE Ethanol in combination with the RIDA ® CUBE SCAN device is suitable to quantify ethanol from matrices representing important alcohol-free liquid food categories.