In the Republic of Moldova, the viticulture industry is a sector with a high economic impact, and the utilization of secondary products from winemak ing rep resents a growing concern regarding environmental sustainability. Win e lees, one of the types of wine waste, is less studied in order to valorize it. Currently it is used in the production of ethyl alcohol, as aggregates in the soil and others. The aim of this study was to characterize from a physico-chemical and microbiological point of view the lees sediments obtained after the primary fermentation of three types of individualized red wines made from auto chthonous grapes varieties. It was found that residual yeasts represent a v aluable raw material containing carbohydrates (from 14.35% ± 0.19% to 25.11% ± 1.51% SU), lipids (from 4.61% ± 0.21% to 9.41% ± 2.04% SU), proteins (from 42.62% ± 1.57% to 77.62% ± 9.14% SU), anthocyanins (from 9.18 ± 0.15 to 22.78 ± 1.60 mg cianid) and beta-glucans (from 12.84% ± 0.01% to 17.42% ± 0.02%). The pH value of wine lees ranges from 3.49 ± 0.0 to 3.083 ± 0.01, the dry matter from 9.62% ± 0.22% to 25.06% ± 0.42% and the ash from 0.03% ± 0.42% to 0.035% ± 0.21%. The microbiological study confirmed the presence of live yeasts of the genus Saccharomyces cerevisiae , which remain active due to the presence of residual sugars and oxygen. The results of the research are promising and encourage the obtaining of new products with special purpose and added value.
Said, Z., Sharma, P., Thi Bich Nhuong, Q., et al. (2023) Intelligent Approaches for Sustainable Management and Valorisation of Food Waste. Bioresource Technology, 377, Article ID: 128952. https://doi.org/10.1016/j.biortech.2023.128952
Otles, S., Despoudi, S., Bucatariu, C. and Kartal, C. (2015) Food Waste Management, Valorization, and Sustainability in the Food Industry. In: Galanakis, C.M., Ed., Food Waste Recovery, Elsevier, Amsterdam, 3-23. https://doi.org/10.1016/B978-0-12-800351-0.00001-8
Chirsanova, A. and Calcatiniuc, D. (2021) The Impact of Food Waste and Ways to Minimize It. Journal of Social Sciences, 4, 128-139. https://doi.org/10.52326/jss.utm.2021.4(1).15
Ungureanu, G., Patras, A., Cara, I.G., et al. (2022) Innovative Recovery of Winemaking Waste for Effective Lead Removal from Wastewater. Agronomy, 12, Article No. 604. https://doi.org/10.3390/agronomy12030604
Spigno, G., Marinoni, L. and Garrido, G.D. (2017) State of the Art in Grape Processing By-Products. In: Galanakis, C.M., Ed., Handbook of Grape Processing By-Products, Elsevier, Amsterdam, 1-27. https://doi.org/10.1016/B978-0-12-809870-7.00001-6
Mostafa, N.A., Farag, A.A., Abo-dief, H.M. and Tayeb, A.M. (2018) Production of Biodegradable Plastic from Agricultural Wastes. Arabian Journal of Chemistry, 11, 546-553. https://doi.org/10.1016/j.arabjc.2015.04.008
Kokkinomagoulos, E. and Kandylis, P. (2023) Grape Pomace, an Undervalued By-Product: Industrial Reutilization within a Circular Economy Vision. Reviews in Environmental Science and Bio/Technology, 22, 739-773. https://doi.org/10.1007/s11157-023-09665-0
Crescente, G., Cascone, G., Petruzziello, A., et al. (2023) A Comparative Study between Microwave Hydrodiffusion and Gravity (MHG) and Ultrasound-Assisted Extraction (UAE): Chemical and Biological Characterization of Polyphenol-Enriched Extracts from Aglianico Grape Pomace. Foods, 12, Article No. 2678. https://doi.org/10.3390/foods12142678
Ravi, H.K., Breil, C., Vian, M.A., Chemat, F. and Venskutonis, P.R. (2018) Biorefining of Bilberry (Vaccinium myrtillus L.) Pomace Using Microwave Hydrodiffusion and Gravity, Ultrasound-Assisted, and Bead-Milling Extraction. ACS Sustainable Chemistry & Engineering, 6, 4185-4193. https://doi.org/10.1021/acssuschemeng.7b04592
The International Organisation of Vine and Wine (Last) (2023) State of the World Vine and Wine Sector in 2022.
Dimou, C., Kopsahelis, N., Papadaki, A., et al. (2015) Wine Lees Valorization: Biorefinery Development Including Production of a Generic Fermentation Feedstock Employed for Poly(3-hydroxybutyrate) Synthesis. Food Research International, 73, 81-87. https://doi.org/10.1016/j.foodres.2015.02.020
Sancho-Galán, P., Amores-Arrocha, A., Jiménez-Cantizano, A. and Palacios, V. (2020) Physicochemical and Nutritional Characterization of Winemaking Lees: A New Food Ingredient. Agronomy, 10, Article No. 996. https://doi.org/10.3390/agronomy10070996
Rakowska, R., Sadowska, A., Dybkowska, E. and Swiderski, F. (2017) Spent Yeast as Natural Source of Functional Food Additives. Roczniki Panstwowego Zakladu Higieny, 68, 115-121.
Oltean, F.D. and Gabor, M.R. (2022) Wine Tourism—A Sustainable Management Tool for Rural Development and Vineyards: Cross-Cultural Analysis of the Consumer Profile from Romania and Moldova. Agriculture, 12, 1614. https://doi.org/10.3390/agriculture12101614
Wine Regions of Moldova. https://wineofmoldova.com/en/wine-regions/
Varelas, V., Tataridis, P., Liouni, M. and Nerantzis, E.T. (2016) Valorization of Winery Spent Yeast Waste Biomass as a New Source for the Production of β-Glucan. Waste and Biomass Valorization, 7, 807-817. https://doi.org/10.1007/s12649-016-9530-4
Morales, D. (2023) Food By-Products and Agro-Industrial Wastes as a Source of β-Glucans for the Formulation of Novel Nutraceuticals. Pharmaceuticals, 16, Article No. 460. https://doi.org/10.3390/ph16030460
Besliu, A., Chiselita, N., Chiselita, O. and Efremova, N. (2022) New Processes for Obtaining Mannoproteins from Beer Yeast Sediments and Their Biochemical Properties. Engineering, Biotechnology, Food Industry, 1, 41-48.
De Iseppi, A., Lomolino, G., Marangon, M. and Curioni, A. (2020) Current and Future Strategies for Wine Yeast Lees Valorization. Food Research International, 137, Article ID: 109352. https://doi.org/10.1016/j.foodres.2020.109352
Ma, J., Sun, Y., Meng, D., et al. (2023) Yeast Proteins: The Novel and Sustainable Alternative Protein in Food Applications. Trends in Food Science & Technology, 135, 190-201. https://doi.org/10.1016/j.tifs.2023.04.003
Branco, P., Maurício, E.M., Costa, A., et al. (2023) Exploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Properties. Antibiotics, 12, Article No. 1332. https://doi.org/10.3390/antibiotics12081332
Mekoue Nguela, J., Teuf, O., Assuncao Bicca, S. and Vernhet, A. (2023) Impact of Mannoprotein N-Glycosyl Phosphorylation and Branching on the Sorption of Wine Polyphenols by Yeasts and Yeast Cell Walls. Food Chemistry, 403, Article ID: 134326. https://doi.org/10.1016/j.foodchem.2022.134326
Resitca, V., Balanuta, A., et al. (2022) Possibility and Necessity of Tartaric Acid Production in the Republic of Moldova. Journal of Engineering Science, 29, 151-163. https://doi.org/10.52326/jes.utm.2022.29(1).14
De Iseppi, A., Marangon, M., Lomolino, G., et al. (2021) Red and White Wine Lees as a Novel Source of Emulsifiers and Foaming Agents. LWT, 152, Article ID: 112273. https://doi.org/10.1016/j.lwt.2021.112273
Di Nicolantonio, L., Ferrati, M., Cristino, M., et al. (2023) Evaluation of Physicochemical and Microbial Properties of Extracts from Wine Lees Waste of Matelica’s Verdicchio and Their Applications in Novel Cosmetic Products. Antioxidants, 12, Article No. 816. https://doi.org/10.3390/antiox12040816
Constantin, O.E. and Istrati, D.I. (2022) Extraction, Quantification and Characterization Techniques for Anthocyanin Compounds in Various Food Matrices—A Review. Horticulturae, 8, Article No. 1084. https://doi.org/10.3390/horticulturae8111084
Breil, C., Abert Vian, M., Zemb, T., et al. (2017) “Bligh and Dyer” and Folch Methods for Solid-Liquid-Liquid Extraction of Lipids from Microorganisms. Comprehension of Solvatation Mechanisms and towards Substitution with Alternative Solvents. International Journal of Molecular Sciences, 18, Article No. 708. https://doi.org/10.3390/ijms18040708
Attard, E. (2013) A Rapid Microtitre Plate Folin-Ciocalteu Method for the Assessment of Polyphenols. Open Life Sciences, 8, 48-53. https://doi.org/10.2478/s11535-012-0107-3
Dey, P.M. and Harborne, J.B. (1993) Methods in Plant Biochemistry. Carbohydrats. Academic Press, Cambridge.
Waterborg, J.H. and Matthews, H.R. (1984) The Lowry Method for Protein Quantitation. In: Walker, J.M., Ed., Proteins, Springer, Berlin, 1-3. https://doi.org/10.1385/0-89603-062-8:1
Hadwan, M.H. (2018) Simple Spectrophotometric Assay for Measuring Catalase Activity in Biological Tissues. BMC Biochemistry, 19, Article No. 7. https://doi.org/10.1186/s12858-018-0097-5
Chiselita, N., Chiselita, O., Besliu, A., et al. (2022) Biochemical Composition and Antioxidant Activity of Different Preparations from Microbial Waste of the Beer Industry. Acta Universitatis Cibiniensis Series E: Food Technology, 26, 139-146. https://doi.org/10.2478/aucft-2022-0011
Besliu, A., Chiselita, N., Chiselita, O., et al. (2022) Biochemical Composition and Antioxidant Activity of the Mannoprotein Preparation Obtained Yeast Biomass from Wine Industry Waste. Notulae Scientia Biologicae, 14, Article No. 11229. https://doi.org/10.55779/nsb14211229
Yuan, H., He, Y., Zhang, H. and Ma, X. (2022) Ultrasound-Assisted Enzymatic Hydrolysis of Yeast β-Glucan Catalyzed by β-Glucanase: Chemical and Microstructural Analysis. Ultrasonics Sonochemistry, 86, Article ID: 106012. https://doi.org/10.1016/j.ultsonch.2022.106012
(2019) Microbiology of the Food Chain Horizontal Method for the Enumeration of Microorganisms. ISO 4833-1:2013.
York, M.K. (2004) Gram Stain. In: Isenberg, H.D., Ed., Clinical Microbiology Procedures Handbook, 2nd Edition, ASM Press, Washington DC.
Er, Y. (2016) The Classification of White Wine and Red Wine According to Their Physicochemical Qualities. International Journal of Intelligent Systems and Applications in Engineering, 4, 23-26. https://doi.org/10.18201/ijisae.265954
Ofoedu, C.E., Ofoedu, E.O., Chacha, J.S., et al. (2022) Comparative Evaluation of Physicochemical, Antioxidant, and Sensory Properties of Red Wine as Markers of Its Quality and Authenticity. International Journal of Food Science, 2022, Article ID: 8368992. https://doi.org/10.1155/2022/8368992
De Andrade Bulos, R.B., Da Gama Paz, F., Machado, C.G., et al. (2023) Scientific and Technological Research on the Use of Wine Lees. Food Production, Processing and Nutrition, 5, Article No. 25. https://doi.org/10.1186/s43014-023-00137-0
Molina-Alcaide, E., Moumen, A. and Martín-García, A.I. (2008) By-Products from Viticulture and the Wine Industry: Potential as Sources of Nutrients for Ruminants. Journal of the Science of Food and Agriculture, 88, 597-604. https://doi.org/10.1002/jsfa.3123
Chong, H.H., Cleary, M.T., Dokoozlian, N., et al. (2019) Soluble Cell Wall Carbohydrates and Their Relationship with Sensory Attributes in Cabernet Sauvignon Wine. Food Chemistry, 298, Article ID: 124745. https://doi.org/10.1016/j.foodchem.2019.05.020
Matteo, B.P. (2016) Valorization of Wine Making By-Products. CRC Press, Boca Raton.
Jach, M.E., Serefko, A., Ziaja, M. and Kieliszek, M. (2022) Yeast Protein as an Easily Accessible Food Source. Metabolites, 12, Article No. 63. https://doi.org/10.3390/metabo12010063
Agboola, J.O., Overland, M., Skrede, A. and Hansen, J.O. (2021) Yeast as Major Protein-Rich Ingredient in Aquafeeds: A Review of the Implications for Aquaculture Production. Reviews in Aquaculture, 13, 949-970. https://doi.org/10.1111/raq.12507
Li, X., Wu, J., Kang, Y., et al. (2022) Yeast Mannoproteins Are Expected to Be a Novel Potential Functional Food for Attenuation of Obesity and Modulation of Gut Microbiota. Frontiers in Nutrition, 9, Article ID: 1019344. https://doi.org/10.3389/fnut.2022.1019344
Jara-Palacios, M.J. (2019) Wine Lees as a Source of Antioxidant Compounds. Antioxidants, 8, Article No. 45. https://doi.org/10.3390/antiox8020045
Costa-Pérez, A., Medina, S., Sánchez-Bravo, P., et al. (2023) The (Poly)phenolic Profile of Separate Winery By-Products Reveals Potential Antioxidant Synergies. Molecules, 28, Article No. 2081. https://doi.org/10.3390/molecules28052081
Zhijing, Y., Shavandi, A., Harrison, R. and Bekhit, A.E.-D. (2018) Characterization of Phenolic Compounds in Wine Lees. Antioxidants, 7, Article No. 48. https://doi.org/10.3390/antiox7040048
Landeka Jurcevic, I., Dora, M., Guberovic, I., et al. (2017) Wine Lees Polyphenols as a Novel Functional Bioactive Compound in the Protection against Oxidative Stress and Hyperlipidemia. Food Technology and Biotechnology, 55, 109-116. https://doi.org/10.17113/ftb.55.01.17.4894
Lamoth, F., Nucci, M., Fernandez-Cruz, A., et al. (2023) Performance of the Beta-Glucan Test for the Diagnosis of Invasive Fusariosis and Scedosporiosis: A Meta-Analysis. Medical Mycology, 61, myad061. https://doi.org/10.1093/mmy/myad061
Wang, M., Zhang, Z., Dong, X. and Zhu, B. (2023) Targeting β-Glucans, Vital Components of the Pneumocystis Cell Wall. Frontiers in Immunology, 14, Article ID: 1094464. https://doi.org/10.3389/fimmu.2023.1094464
Mishra, V., Tripathi, V., Yadav, P. and Singh, M.P. (2023) Beta Glucan as an Immune Stimulant in Tumor Microenvironment—Insight into Lessons and Promises from Past Decade. International Journal of Biological Macromolecules, 234, Article ID: 123617. https://doi.org/10.1016/j.ijbiomac.2023.123617
Keating, S.T. and El-Osta, A. (2023) Metaboloepigenetics in Cancer, Immunity, and Cardiovascular Disease. Cardiovascular Research, 119, 357-370. https://doi.org/10.1093/cvr/cvac058
Genc, H., Ozdemir, M. and Demirbas, A. (2001) Analysis of Mixed-Linked (1→3), (1→4)-β-d-Glucans in Cereal Grains from Turkey. Food Chemistry, 73, 221-224. https://doi.org/10.1016/S0308-8146(00)00290-9
Demirbas, A. (2005) Beta-Glucan and Mineral Nutrient Contents of Cereals Grown in Turkey. Food Chemistry, 90, 773-777. https://doi.org/10.1016/j.foodchem.2004.06.003
Usatii, A. and Chiselita, N. (2016) Solutii inovative de cultivare a levurilor producatoare de β-glucani. Intellectus, No. 4, 77-80.
Sherman, E., Yvon, M., Grab, F., et al. (2023) Total Lipids and Fatty Acids in Major New Zealand Grape Varieties during Ripening, Prolonged Pomace Contacts and Ethanolic Extractions Mimicking Fermentation. Fermentation, 9, Article No. 357. https://doi.org/10.3390/fermentation9040357
Del Río, J.C., Prinsen, P. and Gutiérrez, A. (2013) Chemical Composition of Lipids in Brewer’s Spent Grain: A Promising Source of Valuable Phytochemicals. Journal of Cereal Science, 58, 248-254. https://doi.org/10.1016/j.jcs.2013.07.001
Niemi, P., Tamminen, T., Smeds, A., et al. (2012) Characterization of Lipids and Lignans in Brewer’s Spent Grain and Its Enzymatically Extracted Fraction. Journal of Agricultural and Food Chemistry, 60, 9910-9917. https://doi.org/10.1021/jf302684x
Ascrizzi, R., Pieracci, Y., Melai, B., et al. (2022) Preliminary Study on Red Wine Aroma: The Volatile Profiles of Six Grape Cultivars in Different Vinification Phases. Fermentation, 8, Article No. 753. https://doi.org/10.3390/fermentation8120753
Sabra, A., Netticadan, T. and Wijekoon, C. (2021) Grape Bioactive Molecules, and the Potential Health Benefits in Reducing the Risk of Heart Diseases. Food Chemistry: X, 12, Article ID: 100149. https://doi.org/10.1016/j.fochx.2021.100149
Henderson, C.M. and Block, D.E. (2014) Examining the Role of Membrane Lipid Composition in Determining the Ethanol Tolerance of Saccharomyces cerevisiae. Applied and Environmental Microbiology, 80, 2966-2972. https://doi.org/10.1128/AEM.04151-13
Girardi Piva, G., Casalta, E., Legras, J.-L., et al. (2022) Characterization and Role of Sterols in Saccharomyces cerevisiae during White Wine Alcoholic Fermentation. Fermentation, 8, Article No. 90. https://doi.org/10.3390/fermentation8020090
Worrasinchai, S., Suphantharika, M., Pinjai, S. and Jamnong, P. (2006) β-Glucan Prepared from Spent Brewer’s Yeast as a Fat Replacer in Mayonnaise. Food Hydrocolloids, 20, 68-78. https://doi.org/10.1016/j.foodhyd.2005.03.005
Marinescu, G., Stoicescu, A. and Patrascu, L. (2011) The Preparation of Mayonnaise Containing Spent Brewer’s Yeast β-Glucan as a Fat Replacer. Romanian Biotechnological Letters, 16, 6017-6025.
Bianchi, F., Cervini, M., Giuberti, G. and Simonato, B. (2023) The Potential of Wine Lees as a Fat Substitute for Muffin Formulations. Foods, 12, Article No. 2584. https://doi.org/10.3390/foods12132584
Vasquez Mejia, S.M., de Francisco, A., Manique Barreto, P.L., et al. (2018) Incorporation of β-Glucans in Meat Emulsions through an Optimal Mixture Modeling Systems. Meat Science, 143, 210-218. https://doi.org/10.1016/j.meatsci.2018.05.007
Mykhalevych, A., Polishchuk, G., Nassar, K., et al. (2022) β-Glucan as a Techno-Functional Ingredient in Dairy and Milk-Based Products—A Review. Molecules, 27, Article No. 6313. https://doi.org/10.3390/molecules27196313
Martins, Z.E., Pinho, O. and Ferreira, I. (2018) Impact of New Ingredients Obtained from Brewer’s Spent Yeast on Bread Characteristics. Journal of Food Science and Technology, 55, 1966-1971. https://doi.org/10.1007/s13197-018-3107-0
Caruso, M.A., Piermaria, J.A., Abraham, A.G. and Medrano, M. (2022) β-Glucans Obtained from Beer Spent Yeasts as Functional Food Grade Additive: Focus on Biological Activity. Food Hydrocolloids, 133, Article ID: 107963. https://doi.org/10.1016/j.foodhyd.2022.107963
De Iseppi, A., Marangon, M., Vincenzi, S., et al. (2021) A Novel Approach for the Valorization of Wine Lees as a Source of Compounds Able to Modify Wine Properties. LWT, 136, Article ID: 110274. https://doi.org/10.1016/j.lwt.2020.110274
Chioru, A. and Chirsanova, A. (2023) β-Glucans: Characterization, Extraction Methods, and Valorization. Food and Nutrition Sciences, 14, 963-983. https://doi.org/10.4236/fns.2023.1410061