Pomegranate and sweet orange juice have been rigorously examined in the present study as a substrate in the context of distinctive wine production research. An optimization investigation was conducted utilizing the Box-Behnken Response Surface Method (RSM) design. The influences of fermentation parameters, including the ratio of sweet orange in the mixed fruit juice must (SW), fermentation temperature (TF), inoculum size (IS), and ˚Brix (BX), on total phenolic concentration (TPC), antioxidant activity (AA), total anthocyanin concentration (ACN), and ascorbic acid equivalent (AAE) were systematically assessed. The findings of the study indicated that a 20% proportion of sweet oranges, with a ˚Brix of 23, a fermentation temperature of 26˚C, and an inoculum size of 10%, constituted the optimal conditions for the production of high-quality pomegranate-sweet orange mixed fruit juice wine. The results obtained underscore the potential of sweet orange fruit in the advancement of premium pomegranate-sweet orange wine.
KeywordsPomegranateSweet OrangeRSMFruit JuiceWine
Dutoit, W. and Oberholster, A. (2014) Processing and Impact on Anti-Oxidants in Beverages. Elsevier.
Avellone, G., Di Garbo, V., Campisi, D., De Simone, R., Raneli, G., Scaglione, R., et al . (2005) Effects of Moderate Sicilian Red Wine Consumption on Inflammatory Biomarkers of Atherosclerosis. European Journal of Clinical Nutrition , 60, 41-47. https://doi.org/10.1038/sj.ejcn.1602265
Cordova, A. and Sumpio, B. (2009) Polyphenols Are Medicine: Is It Time to Prescribe Red Wine for Our Patients? International Journal of Angiology , 18, 111-117. https://doi.org/10.1055/s-0031-1278336
NRCP (2008) Annual Report 2007-2008. National Research Centre on Pomegranate, Solapur, Maharashtra, India. 1-23.
Patil, M.B. and Panchal, V.M. (2016) Comparative Studies on ‘Nucellar’, ‘Sathgudi’ and ‘Local’ Sweet Orange (Mosambi) ( Citrus Sinensis Osbeck.) under Marathwada Conditions. Journal of Horticultural Sciences , 11, 44-46. https://doi.org/10.24154/jhs.v11i1.102
Shahrajabian, M.H., Sun, W. and Cheng, Q. (2021) Pomegranate, Fruit of the Desert, a Functional Food, and a Healthy Diet. Notulae Scientia Biologicae , 13, 11085. https://doi.org/10.15835/nsb13311085
Bhardwaj, R.L., Nandal, U., Pal, A. and Jain, S. (2014) Bioactive Compounds and Medicinal Properties of Fruit Juices. Fruits , 69, 391-412. https://doi.org/10.1051/fruits/2014027
Liang, C. (1999) An Industrial Analysis of the United States wine Industry, World Wine Industry, and China Wine Industry. Master’s Thesis, University of Wisconsin—Stout.
Petrova, N. and Stefanova, A. (2020) Theoretical Overview of the Factors Affecting Wine Production. Trakia Journal of Sciences , 18, 619-624. https://doi.org/10.15547/tjs.2020.s.01.099
Market-Growth, G. (2019) Trends, and Forecast (2020-2025). Mordor Intelligence.
Asgary, S., Zarfeshany, A. and Javanmard, S. (2014) Potent Health Effects of Pomegranate. Advanced Biomedical Research , 3, 100. https://doi.org/10.4103/2277-9175.129371
Tsegay, Z.T. and Lemma, S.M. (2020) Response Surface Optimization of Cactus Pear ( Opuntia Ficus - Indica ) with Lantana Camara ( L. camara ) Fruit Fermentation Process for Quality Wine Production. International Journal of Food Science , 2020, Article ID: 8647262. https://doi.org/10.1155/2020/8647262
Bhoite, A.A., Gaikwad, N.N., Sathe, S.J., Banerjee, K. and Dhanshetty, P.M. (2019) Fermentation Studies on Pomegranate and Sweet Orange Blended Juice. Annals Food Science and Technology , 20, 735-745.
Bruns, R.E., Scarmino, I.S. and Barros Neto, B. (2006) Statistical Design—Chemometrics. Elsevier.
Vohra, A. and Satyanarayana, T. (2002) Statistical Optimization of the Medium Components by Response Surface Methodology to Enhance Phytase Production by Pichia Anomala. Process Biochemistry , 37, 999-1004. https://doi.org/10.1016/s0032-9592(01)00308-9
Harris, L.J. and Ray, S.N. (1935) Determination of Plasma Ascorbic Acid by 2, 6-Dichlorophenol Indophenol Titration.
Singleton, V.L. and Rossi, J.A. (1965) Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture , 16, 144-158. https://doi.org/10.5344/ajev.1965.16.3.144
Wrolstad, R.E., Durst, R.W. and Lee, J. (2005) Tracking Color and Pigment Changes in Anthocyanin Products. Trends in Food Science & Technology , 16, 423-428. https://doi.org/10.1016/j.tifs.2005.03.019
Benzie, I.F.F. and Strain, J.J. (1996) The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry , 239, 70-76. https://doi.org/10.1006/abio.1996.0292
Montgomery, D.C. (2017) Design and Analysis of Experiments. John Wiley & Sons.
Peng, Z., Duncan, B., Pocock, K.F. and Sefton, M.A. (1998) The Effect of Ascorbic Acid on Oxidative Browning of White Wines and Model Wines. Australian Journal of Grape and Wine Research , 4, 127-135. https://doi.org/10.1111/j.1755-0238.1998.tb00141.x
Tchabo, W., Ma, Y., Kwaw, E., Zhang, H. and Li, X. (2017) Influence of Fermentation Parameters on Phytochemical Profile and Volatile Properties of Mulberry ( Morus nigra ) Wine. Journal of the Institute of Brewing , 123, 151-158. https://doi.org/10.1002/jib.401
Valentine, G.D.S., Walker, M.E., Gardner, J.M., Schmid, F. and Jiranek, V. (2018) Brief Temperature Extremes during Wine Fermentation: Effect on Yeast Viability and Fermentation Progress. Australian Journal of Grape and Wine Research , 25, 62-69. https://doi.org/10.1111/ajgw.12365
Plamada, D., Nemes, A.S., Teleky, B.E., Pascuta, M.S., Odocheanu, R., Mitrea, L., et al . (2024) Microbial Production of Aromatic Phenolic Compounds. In: Jafari, S.M., Harzevili, F.D., Eds., Microbial Production of Food Bioactive Compounds , Springer, 1-24. https://doi.org/10.1007/978-3-030-81403-8_53-1
Messens, W. and De Vuyst, L. (2002) Inhibitory Substances Produced by Lactobacilli Isolated from Sourdoughs—A Review. International Journal of Food Microbiology , 72, 31-43. https://doi.org/10.1016/s0168-1605(01)00611-0
Othman, N.B., Roblain, D., Chammen, N., Thonart, P. and Hamdi, M. (2009) Antioxidant Phenolic Compounds Loss during the Fermentation of Chétoui Olives. Food Chemistry , 116, 662-669. https://doi.org/10.1016/j.foodchem.2009.02.084
Ng, C., Wang, C., Wang, Y., Tzeng, W. and Shyu, Y. (2011) Lactic Acid Bacterial Fermentation on the Production of Functional Antioxidant Herbal Anoectochilus formosanus Hayata. Journal of Bioscience and Bioengineering , 111, 289-293. https://doi.org/10.1016/j.jbiosc.2010.11.011
Zhou, K. and Yu, L. (2004) Effects of Extraction Solvent on Wheat Bran Antioxidant Activity Estimation. LWT — Food Science and Technology , 37, 717-721. https://doi.org/10.1016/j.lwt.2004.02.008
Lee, J., Kang, T.H., Um, B.H., Sohn, E., Han, W., Ji, S., et al . (2013) Evaluation of Physicochemical Properties and Fermenting Qualities of Apple Wines Added with Medicinal Herbs. Food Science and Biotechnology , 22, 1039-1046. https://doi.org/10.1007/s10068-013-0181-y
Li, Z., Teng, J., Lyu, Y., Hu, X., Zhao, Y. and Wang, M. (2018) Enhanced Antioxidant Activity for Apple Juice Fermented with Lactobacillus Plantarum ATCC14917. Molecules , 24, 51. https://doi.org/10.3390/molecules24010051
Gan, R., Shah, N.P., Wang, M., Lui, W. and Corke, H. (2016) Fermentation Alters Antioxidant Capacity and Polyphenol Distribution in Selected Edible Legumes. International Journal of Food Science & Technology , 51, 875-884. https://doi.org/10.1111/ijfs.13062
Călinoiu, L.F., Cătoi, A. and Vodnar, D.C. (2019) Solid-state Yeast Fermented Wheat and Oat Bran as a Route for Delivery of Antioxidants. Antioxidants , 8, Article 372. https://doi.org/10.3390/antiox8090372
Maury, C., Clark, A.C. and Scollary, G.R. (2010) Determination of the Impact of Bottle Colour and Phenolic Concentration on Pigment Development in White Wine Stored under External Conditions. Analytica Chimica Acta , 660, 81-86. https://doi.org/10.1016/j.aca.2009.11.048
Oancea, S., Stoia, M. and Coman, D. (2012) Effects of Extraction Conditions on Bioactive Anthocyanin Content of Vaccinium corymbosum in the Perspective of Food Applications. Procedia Engineering , 42, 489-495. https://doi.org/10.1016/j.proeng.2012.07.440
Watanabe, Y., Yoshimoto, K., Okada, Y. and Nomura, M. (2011) Effect of Impregnation Using Sucrose Solution on Stability of Anthocyanin in Strawberry Jam. LWT — Food Science and Technology , 44, 891-895. https://doi.org/10.1016/j.lwt.2010.11.003
Sui, X., Dong, X. and Zhou, W. (2014) Combined Effect of pH and High Temperature on the Stability and Antioxidant Capacity of Two Anthocyanins in Aqueous Solution. Food Chemistry , 163, 163-170. https://doi.org/10.1016/j.foodchem.2014.04.075
He, F., Liang, N., Mu, L., Pan, Q., Wang, J., Reeves, M.J., et al . (2012) Anthocyanins and Their Variation in Red Wines I. Monomeric Anthocyanins and Their Color Expression. Molecules , 17, 1571-1601. https://doi.org/10.3390/molecules17021571
Marquez, A., Serratosa, M.P. and Merida, J. (2013) Pyranoanthocyanin Derived Pigments in Wine: Structure and Formation during Winemaking. Journal of Chemistry , 2013, Article ID: 713028. https://doi.org/10.1155/2013/713028
Morata, A., Gómez-Cordovés, M.C., Calderón, F. and Suárez, J.A. (2006) Effects of pH, Temperature and SO 2 on the Formation of Pyranoanthocyanins during Red Wine Fermentation with Two Species of Saccharomyces . International Journal of Food Microbiology , 106, 123-129. https://doi.org/10.1016/j.ijfoodmicro.2005.05.019
María, M. and Begona, B. (2009) Anthocyanins and Anthocyanin Derived Compounds. In: Moreno-Arribas, M.V.P. and Carmen, M., Eds., Wine Chemistry and Biochemistry , Springer, 439-462.
Peng, B., Lei, Y., Zhao, H. and Cui, L. (2015) Response Surface Methodology for Optimization of Fermentation Process Parameters for Improving Apple Wine Quality. Journal of Food Science and Technology , 52, 7513-7518. https://doi.org/10.1007/s13197-015-1872-6
Schvab, M.D.C., Ferreyra, M.M., Davies, C.V., Stefani, A., Cayetano, M.C., Gerard, L.M., et al . (2015) Effects of Orange Winemaking Variables on Antioxidant Activity and Bioactive Compounds. Food Science and Technology , 35, 407-413. https://doi.org/10.1590/1678-457x.6571
Hsu, H., Tsai, Y., Fu, C. and Wu, J.S. (2012) Degradation of Ascorbic Acid in Ethanolic Solutions. Journal of Agricultural and Food Chemistry , 60, 10696-10701. https://doi.org/10.1021/jf3032342
Chuang, P., Shen, S. and Wu, J.S. (2011) Browning in Ethanolic Solutions of Ascorbic Acid and Catechin. Journal of Agricultural and Food Chemistry , 59, 7818-7824. https://doi.org/10.1021/jf200817e
Jagadeesh, U., Mythra, R., Prathima, M.N., Bhagyashree, K.B., Ashoka, S., Srikanth, G.S., et al . (2022) Effect of Fermentation Period on Alcohol Content, pH, TSS and Titrable Acidity during Microbial Processing of Coconut Water for the Development of Coconut Wine. Asian Journal of Dairy and Food Research . https://doi.org/10.18805/ajdfr.dr-1962