Evaluation of the Toxicity of Leaves of Five Cassava Accessions Collected at SAMBA in Ombella M’Poko in the Central African Republic — Oak Academic Publishing
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Evaluation of the Toxicity of Leaves of Five Cassava Accessions Collected at SAMBA in Ombella M’Poko in the Central African Republic
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Laboratory of the Higher Institute of Rural Development of M’Baiki, M’Baiki, Central African Republic
,
Ministry of Health and Population, Bangui, Central African Republic
,
Biological Laboratory of the Pasteur Institute of Bangui, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Lavoisier Hydrosciences Laboratory, Faculty of Sciences, University of Bangui, Bangui, Central African Republic
,
Biological Laboratory of the Pasteur Institute of Bangui, Bangui, Central African Republic
,
National Laboratory of Clinical Biology and Public Health of Bangui, Bangui, Central African Republic
,
National Laboratory of Clinical Biology and Public Health of Bangui, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Biological Laboratory of the Pasteur Institute of Bangui, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
,
National Laboratory of Clinical Biology and Public Health of Bangui, Bangui, Central African Republic
,
Ministry of Health and Population, Bangui, Central African Republic
,
National Laboratory of Clinical Biology and Public Health of Bangui, Bangui, Central African Republic
,
Lavoisier Hydrosciences Laboratory, Faculty of Sciences, University of Bangui, Bangui, Central African Republic
,
Central and West African Virus Epidemiology (WAVE) for Food Security, University of Felix Houphouet-Boigny, Abidjan, Côte d’Ivoire
,
Central and West African Virus Epidemiology (WAVE) for Food Security, University of Felix Houphouet-Boigny, Abidjan, Côte d’Ivoire
1 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
2 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
3 Laboratory of the Higher Institute of Rural Development of M’Baiki, M’Baiki, Central African Republic
4 Ministry of Health and Population, Bangui, Central African Republic
5 Biological Laboratory of the Pasteur Institute of Bangui, Bangui, Central African Republic
6 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
7 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
8 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
9 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
10 Lavoisier Hydrosciences Laboratory, Faculty of Sciences, University of Bangui, Bangui, Central African Republic
11 Biological Laboratory of the Pasteur Institute of Bangui, Bangui, Central African Republic
12 National Laboratory of Clinical Biology and Public Health of Bangui, Bangui, Central African Republic
13 National Laboratory of Clinical Biology and Public Health of Bangui, Bangui, Central African Republic
14 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
15 Biological Laboratory of the Pasteur Institute of Bangui, Bangui, Central African Republic
16 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
17 Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
Laboratory of Biological and Agronomic Sciences for Development, Faculty of Sciences, Bangui, Central African Republic
Cassava ( Manihot esculenta ) is a perennial shrub of the Euphorbiaceae family. Thus, the objectives assigned to this study are to evaluate the toxicity of the leaves of 5 cassava accessions collected in Samba using mice of the Wistar albino provided by the Pasteur Institute of Bangui. The aim was to evaluate the biochemical parameters and the effects of cyanide on the vital organs of mice in the experiment. Leaf powders from five cassava accessions with concentrations, respectively: 100.66 mg/kg (ICRA14), 103.33 mg/kg (ICRA51), 134.66 mg/kg (MAMBERE), 135.1 mg/kg (ICRA21), and 144 mg/kg (DOGBO) considered higher than the FAO standard (10 mg/kg), were incorporated into the standard diet with a proportion of 25% of the leaf powders from the cassava accessions and 75% of the standard diet. These different food formulations were given daily for 14 days to white mice divided into 6 groups of 6 and a control group (exclusively the standard diet). At the end of the experiment, the mice were sacrificed, and blood and vital organs were taken for various tests, including biochemical tests (ASAT, ALAT, urea, creatinine) as well as histopathological examination of vital organs (liver, kidneys, heart). The results show a very significant elevation of liver and kidney markers, accompanied by weight loss and visible changes in the organs examined, highlighting the presence of atrophy, dystrophy, ischemia, and necrosis in the livers, kidneys, and hearts of the mice tested, except for the control group. These results also show that the accessions studied, DOGBO, ICRA21, MAMBERE, ICRA51, and ICRA14, have considerable toxic potential. This information is crucial and raises awareness as a prelude to the solutions to be considered. It is therefore crucial to carry out a thorough assessment before any consumption of leaves and derived products, particularly by-products, in the Central African Republic.
Ufuan Achidi, A., Ajayi, O.A., Bokanga, M. and Maziya-Dixon, B. (2005) The Use of Cassava Leaves as Food in Africa. Ecology of Food and Nutrition , 44, 423-435. https://doi.org/10.1080/03670240500348771
Jennings, D.L. and Iglesias, C. (2001) Breeding for Crop Improvement. In: Cassava : Biology, Production and Utilization , CABI Publishing, 149-166. https://doi.org/10.1079/9780851995243.0149
McMahon, J.M., White, W.L.B. and Sayre, R.T. (1995) Review Article: Cyanogenesis in Cassava ( Manihot esculenta Crantz). Journal of Experimental Botany , 46, 731-741. https://doi.org/10.1093/jxb/46.7.731
Kerharo, J. and Bouquet, A. (1950) Medicinal and Toxic Plants of Ivory Coast and Upper Volta. Vigot Brothers.
Keita, Å.V., Lindqvist, C.M., Öst, Å., Magana, C.D.L., Schoultz, I. and Halfvarson, J. (2018) Gut Barrier Dysfunction—A Primary Defect in Twins with Crohn’s Disease Predominantly Caused by Genetic Predisposition. Journal of Crohn ’ s and Colitis , 12, 1200-1209. https://doi.org/10.1093/ecco-jcc/jjy045
Cliff, J., Mårtensson, J., Lundqvist, P., Rosling, H. and Sörbo, B. (1985) Association of High Cyanide and Low Sulphur Intake in Cassava-Induced Spastic Paraparesis. The Lancet , 326, 1211-1213. https://doi.org/10.1016/s0140-6736(85)90742-1
Tylleskär, T., Rosling, H., Banea, M., Bikangi, N., Cooke, R.D. and Poulter, N.H. (1992) Cassava Cyanogens and Konzo, an Upper Motoneuron Disease Found in Africa. The Lancet , 339, 208-211. https://doi.org/10.1016/0140-6736(92)90006-o
FAOSTAT (2016) 2017 Food and Agriculture Organization of the United Nations. https://fr.wikipedia.org/wiki/Organisation_des_Nations_unies_pour_l%27alimentation_et_l%27agricculture
Tylleskär, T., Banea, M., Bikangi, N., Fresco, L., Persson, L.A. and Rosling, H. (1991) Epidemiological Evidence from Zaire for a Dietary Etiology of Konzo, an Upper Motor Neuron Disease. Bulletin of the World Health Organization , 69, 581-589.
Zinga, I., Chiroleu, F., Legg, J., Lefeuvre, P., Komba, E.K., Semballa, S., et al . (2013) Epidemiological Assessment of Cassava Mosaic Disease in Central African Republic Reveals the Importance of Mixed Viral Infection and Poor Health of Plant Cuttings. Crop Prot ection , 44, 6-12. https://doi.org/10.1016/j.cropro.2012.10.010
Bolhuis, G.G. (1954) The Toxicity of Cassava Roots. Netherlands Journal of Agricult ural Science , 2, 176-185. https://doi.org/10.18174/njas.v2i3.17841
18
19 National Laboratory of Clinical Biology and Public Health of Bangui, Bangui, Central African Republic
20 Ministry of Health and Population, Bangui, Central African Republic
21 National Laboratory of Clinical Biology and Public Health of Bangui, Bangui, Central African Republic
22 Lavoisier Hydrosciences Laboratory, Faculty of Sciences, University of Bangui, Bangui, Central African Republic
23 Central and West African Virus Epidemiology (WAVE) for Food Security, University of Felix Houphouet-Boigny, Abidjan, Côte d’Ivoire
24 Central and West African Virus Epidemiology (WAVE) for Food Security, University of Felix Houphouet-Boigny, Abidjan, Côte d’Ivoire
Tisserant, C. (1953) Agriculture in the Savannahs of Oubangui. Bulletin of the Inst itute of Central African Studies ( Brazzaville ), New Series , 6, 27.
Ignaleamoko, E.F., Zinga, I., Semballa, S., Longue, D.R.S., Sako, E.P., Fulgence, I.J., et al . (2025) Evaluation of the Hydrocyanic Acid Level in Fresh Cassava Leaves: Case of the Experimental Field in the Ombella M’Poko in the Central African Republic. American Journal of Plant Sciences , 16, 525-536. https://doi.org/10.4236/ajps.2025.165039
Nguerepende, O.S., Zinga, I., Mebourou, E.K., Valam, A., Semballa, S., Namkoune, E.R., et al . (2025) Effect of Cassava ( Manihot esculenta Crantz) Leaves Infected with Mosaic Virus on the Growth and Organs of Mice. Journal of Food and Nutrition Sciences , 13, 1-9.
Rhiouani, H., El-Hilaly, J., Israili, Z.H. and Lyoussi, B. (2008) Acute and Sub-Chronic Toxicity of an Aqueous Extract of the Leaves of Herniaria Glabra in Rodents. Journal of Ethnopharmacology , 118, 378-386. https://doi.org/10.1016/j.jep.2008.05.009
Lakmichi, H., Bakhtaoui, F.Z., Gadhi, C.A., Ezoubeiri, A., El Jahiri, Y., El Mansouri, A., et al . (2010) Toxicity Profile of the Aqueous Ethanol Root Extract of Corrigiola telephiif olia Pourr. (Caryophyllaceae) in Rodents. Evidence - Based Compleme ntary and Alternative Medicine , 2011, Article ID: 317090. https://doi.org/10.1155/2011/317090
Hilaly, J.E., Israili, Z.H. and Lyoussi, B. (2004) Acute and Chronic Toxicological Studies of Ajuga Iva in Experimental Animals. Journal of Ethnopharmacology , 91, 43-50. https://doi.org/10.1016/j.jep.2003.11.009
Betti, A.H., Stein, A.C., Dallegrave, E., Wouters, A.T.B., Watanabe, T.T.N., Driemeier, D., et al . (2012) Acute and Repeated-Doses (28 Days) Toxicity Study of Hypericum polyanthemum Klotzsch ex Reichardt (Guttiferare) in Mice. Food and Chemical Toxicology , 50, 2349-2355. https://doi.org/10.1016/j.fct.2012.04.012
Mukinda, J.T. and Syce, J.A. (2007) Acute and Chronic Toxicity of the Aqueous Extract of Artemisia Afra in Rodents. Journal of Ethnopharmacology , 112, 138-144. https://doi.org/10.1016/j.jep.2007.02.011
Li, X., Luo, Y., Wang, L., Li, Y., Shi, Y., Cui, Y., et al . (2010) Acute and Subacute Toxicity of Ethanol Extracts from Salvia Przewalskii Maxim in Rodents. Journal of Ethnopharmacology , 131, 110-115. https://doi.org/10.1016/j.jep.2010.06.012
Olson, H., Betton, G., Robinson, D., Thomas, K., Monro, A., Kolaja, G., et al . (2000) Concordance of the Toxicity of Pharmaceuticals in Humans and in Animals. Regul atory Toxicology and Pharmacology , 32, 56-67. https://doi.org/10.1006/rtph.2000.1399
Hariri, A.T., Moallem, S.A., Mahmoudi, M. and Hosseinzadeh, H. (2011) The Effect of Crocin and Safranal, Constituents of Saffron, against Subacute Effect of Diazinon on Hematological and Genotoxicity Indices in Rats. Phytomedicine , 18, 499-504. https://doi.org/10.1016/j.phymed.2010.10.001
Atsamo, A.D., Nguelefack, T.B., Datté, J.Y. and Kamanyi, A. (2011) Acute and Subchronic Oral Toxicity Assessment of the Aqueous Extract from the Stem Bark of Erythrina Senegalensis DC (Fabaceae) in Rodents. Journal of Ethnopharmacology , 134, 697-702. https://doi.org/10.1016/j.jep.2011.01.023
Diaby, V., Yapo, A.F., Adon, A.M., Yapi, H.F., Djama, A.J. and Dosso, M. (2017) Biotoxicité hématologique du sulfate de cadmium chez les rats Wistar. International Journal of Biological and Chemical Sciences , 10, 1765-1772. https://doi.org/10.4314/ijbcs.v10i4.25
Barlow, J.B., Giboney, J.S., Keith, M.J., Wilson, D.W., Schuetzler, R.M., Lowry, P.B., et al . (2011) Overview and Guidance on Agile Development in Large Organizations. Communications of the Association for Information Systems , 29, 25-44. https://doi.org/10.17705/1cais.02902
Wallace, A.D. and Meyer, S.A. (2010) Hepatotoxicity. In: Hodgson, E., Ed., A Textb ook of Modern Toxicology (4 th Ed ition ), John Wiley & Sons, 277-290.
Adeneye, A.A., Ajagbonna, O.P., Adeleke, T.I. and Bello, S.O. (2006) Preliminary Toxicity and Phytochemical Studies of the Stem Bark Aqueous Extract of Musanga cec ropioides in Rats. Journal of Ethnopharmacology , 105, 374-379. https://doi.org/10.1016/j.jep.2005.11.027
Jodynis-Liebert, J., Nowicki, M., Murias, M., Adamska, T., Ewertowska, M., Kujawska, M., et al . (2010) Cytotoxicity, Acute and Subchronic Toxicity of Ionic Liquid, Didecyldimethylammonium Saccharinate, in Rats. Regulatory Toxicology and Pharmacology , 57, 266-273. https://doi.org/10.1016/j.yrtph.2010.03.006
Mojirayo, R.I. (2017) In Vivo Anti-Plasmodial Activity and Histopathological Analysis of Water and Ethanol Extracts of a Polyherbal Antimalarial Recipe. Journal of Pharmacognosy and Phytotherapy , 9, 87-100. https://doi.org/10.5897/jpp2017.0449
Kumar, K., Sharma, S., Kumar, A., Bhardwaj, P., Barhwal, K. and Hota, S.K. (2017) Acute and Sub-Acute Toxicological Evaluation of Lyophilized Nymphaea × rubra Roxb. ex Andrews Rhizome Extract. Regulatory Toxicology and Pharmacology , 88, 12-21. https://doi.org/10.1016/j.yrtph.2017.04.008
Timbrell, J. (2001) Principles of Biochemical Toxicology. 3rd Edition, Taylor & Francis, 1-390.